We narrowed to 23,099 results for: crispr

1. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAH01Sp::Cas9D10A

   Plasmid

   #128161

   Purpose

   Ptet-controlled expression of S. pyogenes Cas9D10A variant

   Depositor

   Calvin Henard

   Article

   Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

   Insert

   Sp Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A

   Promoter

   Ptet

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pECNUS_MP02

   Plasmid

   #194351

   Purpose

   CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

   Depositor

   Eunyoung Chae

   Article

   Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

   Insert

   pcoCas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEntr_PE2

   Plasmid

   #149548

   Purpose

   Gateway entry clone with PE2 enzyme (with stop codon)

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR; Gateway shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSW21

   Plasmid

   #86836

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA4

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Tags

   Expression

   Mutation

   Promoter

   vasa

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB1609_pRR-EGFP

    Plasmid

    #65852

    Purpose

    EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

    Depositor

    Marc Bühler

    Article

    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

    Insert

    split EGFP coding sequence
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSFDuet1-sgRNA(siteT9)

    Plasmid

    #154832

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteT9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAS9-mCherry-TUBB

    Plasmid

    #66942

    Purpose

    CRISPaint target selector TUBB

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA TUBB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMB1-A-pBAD-dCas9

    Plasmid

    #190132

    Purpose

    Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH66

    Plasmid

    #131765

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMB1-A-ptet-dCas9

    Plasmid

    #190129

    Purpose

    Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJH019_SC101_pLAC_Cas12c(4)_Kan

    Plasmid

    #183071

    Purpose

    Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFH54

    Plasmid

    #128183

    Purpose

    Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAN-PA4-RFP

    Plasmid

    #62250

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA4

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRAU171

    Plasmid

    #86835

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA2

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p426*-LbSgH

    Plasmid

    #136992

    Purpose

    Helper plasmid for LbCpf1 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMpGE010-gR082

    Plasmid

    #188553

    Purpose

    Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    gR082
    

    Use

    CRISPR; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCJH021_SC101_pLAC_Cas12c2_Kan

    Plasmid

    #183072

    Purpose

    Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Cas9m2

    Plasmid

    #47314

    Purpose

    Cas9 D10A+H840A

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAN-PA2-RFP

    Plasmid

    #62248

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA2

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFH55

    Plasmid

    #125814

    Purpose

    Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-PmCDA, Arabidopsis codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. DmU6 3p3 GFP LgRNA

    Plasmid

    #182207

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3XP3-GFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFH45

    Plasmid

    #125810

    Purpose

    Level0 nSpCas9-ABE7.10 base changer, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nSpCas9-ABE7.10 base changer, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pICSL90016

    Plasmid

    #117514

    Purpose

    Cas9_2 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_2:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    NLS_SV40

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMAZ-donor

    Plasmid

    #112359

    Purpose

    CRISPR donor plasmid to tag human transcription factor MAZ with GFP

    Depositor

    Kevin White

    Insert

    MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:16:29809765:G>A

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. U6>XX

    Plasmid

    #87542

    Purpose

    Component 1 of 2 for U6>sgRNA cassette OSO-PCR

    Depositor

    Lionel Christiaen

    Article

    Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFH67

    Plasmid

    #131766

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAIO3

    Plasmid

    #137844

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFH32

    Plasmid

    #125809

    Purpose

    Level0 SpCas9-NG, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9-NG, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 6xHis-MBP-huHkCas12a

    Plasmid

    #174683

    Purpose

    Expresses 6xHis-MBP tagged HkCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huHkCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pFH58

    Plasmid

    #128184

    Purpose

    Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pFH24

    Plasmid

    #131763

    Purpose

    Level0 SpCas9, wheat codon optimized (version 2)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized, version 2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFH23

    Plasmid

    #128180

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFH53

    Plasmid

    #128182

    Purpose

    Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pFH92

    Plasmid

    #125820

    Purpose

    Level0 nScCas9-ABE7.10, wheat/human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nScCas9-ABE7.10, wheat/human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pJUNB-donor

    Plasmid

    #112313

    Purpose

    CRISPR donor plasmid to tag human transcription factor JUNB with GFP

    Depositor

    Kevin White

    Insert

    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…

    Promoter

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Cas9m3

    Plasmid

    #47315

    Purpose

    Cas9 D10A+D839A+H840A

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pHEY1-donor

    Plasmid

    #112297

    Purpose

    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP

    Depositor

    Kevin White

    Insert

    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:8:79765311:G>A

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pFH52

    Plasmid

    #128181

    Purpose

    Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge