We narrowed to 10,445 results for: T7

1. (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA

   Plasmid

   #168098

   Purpose

   Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   Nup62-Ubc-Flag-EPEA
   

   Use

   Tags

   EPEA-tag, Flag-tag, and Ubc-Tag

   Expression

   Mammalian

   Mutation

   P229A, S283T on Nup62 native sequence

   Promoter

   T7/cmv

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BRCA1-(1316-1538)

   Plasmid

   #35079

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 1316-1538

   Promoter

   T7

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

3. BRCA1-(498-663)

   Plasmid

   #35070

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 498-663

   Promoter

   T7

   Available since

   April 6, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

   Plasmid

   #101202

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   M1–N497, GGS, V713–D1368

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET21-pelB-LaG-2

   Plasmid

   #172739

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Plasmid

   #101233

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G582A/V583A/E584A/D585A/N588A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G582A, V583A, E584A, D585A and N588A

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

   Plasmid

   #168094

   Purpose

   Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   Nup62-Ubc-Flag-EPEA
   

   Use

   Tags

   BC2-Tag, EPEA-tag, and Flag-tag

   Expression

   Mammalian

   Mutation

   P229A, S283T on Nup62 native sequence

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

   Plasmid

   #138983

   Purpose

   IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

   Plasmid

   #138990

   Purpose

   IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRB (Tcrb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

    Plasmid

    #138989

    Purpose

    IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

    Plasmid

    #138988

    Purpose

    IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

    Plasmid

    #138987

    Purpose

    IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

    Plasmid

    #138986

    Purpose

    IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

    Plasmid

    #138985

    Purpose

    IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRA (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

    Plasmid

    #138984

    Purpose

    IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    TCRB (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. BRCA1-(540-758)

    Plasmid

    #35071

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 540-758

    Promoter

    T7

    Available since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

18. Zebrafish-gRNA-0001

    Plasmid

    #42241

    Purpose

    gRNA targeted to zebrafish gene apoea

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-apoea (apoea Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

19. PMYT1_HUMAN_D0

    Plasmid

    #79687

    Purpose

    This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PMYT1
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET30a-ASXL1-HARE-HTH domain

    Plasmid

    #107164

    Purpose

    Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    ASXL1 (Asxl1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Contains amino acids 2-83

    Promoter

    T7

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Plasmid

    #101236

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K918A/V922A/R925A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K918A, V922A, R925A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid

    #84631

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    ERKsub(TA)DD-Venus-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Mutated target Thr residue to Ala.

    Promoter

    CMV

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-22HT-MSI1(7-103)

    Plasmid

    #60357

    Purpose

    Express the RRM1 of mouse MSI1 in bacterial cells for protein purification

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-103) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    contains amino acids 7-103 of Msi1

    Promoter

    T7

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. BRCA1-(360-498)

    Plasmid

    #35068

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-360

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

27. FN(9-10)-I27-CC

    Plasmid

    #85394

    Purpose

    MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BRCA1-(758-1057)

    Plasmid

    #35085

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-1057

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pet28a_RBPMS_FL

    Plasmid

    #59391

    Purpose

    Plasmid for bacterial overexpression of RBPMS

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p1585588

    Plasmid

    #62620

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    dihydroflavonol 4-reductase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HOXD4 (murine) HIS-tag pET

    Plasmid

    #8548

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by in vitro transcri…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

32. pCDF11-SFPQ(369-598)

    Plasmid

    #135438

    Purpose

    Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    splicing factor proline and glutamine rich (SFPQ Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-28a-His6-SUMO-RABIF

    Plasmid

    #135556

    Purpose

    Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CSK21_HUMAN_D0

    Plasmid

    #79707

    Purpose

    This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, Nicholas Levinson, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK21
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

35. BRCA1-(663-894)

    Plasmid

    #35072

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 663-894

    Promoter

    T7

    Available since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

36. BRCA1-(758-936)

    Plasmid

    #35073

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-936

    Promoter

    T7

    Available since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

37. BRCA1-(408-540)

    Plasmid

    #35069

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 408-540

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

38. BRCA1-(219-758)

    Plasmid

    #35081

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-758

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pACYC-mTET1-N

    Plasmid

    #81056

    Purpose

    bacterial expression of N-terminus of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    N-terminus, amino acids 301-1366

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. BRCA1-(1443-1649)

    Plasmid

    #35080

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1443-1649

    Promoter

    T7

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only