We narrowed to 27,277 results for: Tat

1. pCR3-vsv-CenpB 1-166

   Plasmid

   #108474

   Purpose

   expression of vsv-CenpB 1-166

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB 1-166 AA (CENPB Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   encodes only the DNA-binding domain of CENP-B

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCR3-vsv-Mis12-dCEN-Incenp

   Plasmid

   #108477

   Purpose

   expression of vsv-Mis12-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   Mis12-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by Mis12

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD2038

   Plasmid

   #101378

   Purpose

   Name: pBluescript-ΔRRE. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE.

   Depositor

   Dirk Dittmer

   Article

   Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

   Insert

   Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), RRE
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Blue Heron Biotechnology Inc. bidirectional mutan…

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh-664

   Plasmid

   #92252

   Purpose

   Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   EF1A

   Available since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh-658

   Plasmid

   #92257

   Purpose

   Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRG245-Ras-related

   Plasmid

   #99079

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Ras-related (also known as h.18.4a)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Ras-related (also known as h.18.4a) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOPBP1 G2.4 gRNA

   Plasmid

   #90917

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOPBP1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TOPBP1 (Guide Designation G2.4)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CDC45 B9.2 gRNA

   Plasmid

   #90594

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDC45

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDC45 (Guide Designation B9.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CDC45 B10.2 gRNA

   Plasmid

   #90595

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDC45

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDC45 (Guide Designation B10.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TUBE1 F5.4 gRNA

    Plasmid

    #90928

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TUBE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    TUBE1 (Guide Designation F5.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2-MCT2Prom2.5

    Plasmid

    #87351

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    2.5 kbp proximal promoter of human MCT2 (nested d…

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL2-MCT2Prom1.1

    Plasmid

    #87352

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    1.1 kbp proximal promoter of human MCT2 (nested d…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2-MCT2Prom3.2

    Plasmid

    #87349

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    3.2 kbp proximal promoter of MCT2 (nested deletio…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneoRL-BCL2 3'UTR-mir200c-mut

    Plasmid

    #84598

    Purpose

    Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    BCL2 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on BCL2 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCIneoRL-TOB1 3'UTR-mir200c-mut

    Plasmid

    #84590

    Purpose

    Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    TOB1 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on TOB1 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCIneoRL-RanBP93'UTR-mir200c-mut

    Plasmid

    #84592

    Purpose

    Renilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    RANBP9 3'UTR (RANBP9 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on RANBP9 3…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pVH003

    Plasmid

    #80397

    Purpose

    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments

    Depositor

    Richard Murray

    Article

    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

    Inserts

    Anti-scaffold - Venus YFP
    CusR (cusR )
    AraC (araC )
    

    Use

    Synthetic Biology

    Tags

    Fused by GS linker to LZx domain and Venus has N-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-CAG-Mbd3b-pA-pgk-bsd

    Plasmid

    #74920

    Purpose

    Mammalian expression of Mbd3b

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Insert

    Mnd3b (Mbd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pPB-CAG-Mbd3c-pA-pgk-bsd

    Plasmid

    #74921

    Purpose

    Mammalian expression of Mbd3c

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Insert

    Mbd3c (Mbd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pES006 (pTet-qacR 2/pQacA-deGFP)

    Plasmid

    #69035

    Purpose

    reporter with qacR 2 on single plasmid

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Inserts

    qacR (qacR Synthetic)
    deGFP
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

    Promoter

    pQacA and pTet

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET151-hUCH37 (ISF1)(M148D/F149D)

    Plasmid

    #71388

    Purpose

    Expression of hUCH37 (M148D/F149D) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
    

    Use

    Tags

    HIS-TEV sequence

    Expression

    Bacterial

    Mutation

    changed methionine 148 to aspartate, phenylalanin…

    Promoter

    T7

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

22. Hy_pMT Laccase2 MCS-Laccase2 608-1097

    Plasmid

    #69890

    Purpose

    Expresses the Drosophila laccase2 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    L158I in laccase2

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET30Xa-SBL1(160E)

    Plasmid

    #66846

    Purpose

    Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available since

    Sept. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CFP(159-238) in pcDNAI/Amp

    Plasmid

    #60896

    Purpose

    This vector attaches CFP(159-238) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    CFP(159-238)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Asn-164 to His

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A

    Plasmid

    #65660

    Purpose

    FLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    changed glutamic acid 97 to alanine, lysine 100 t…

    Promoter

    CMV, 2x Tet

    Available since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-N1-hRIP1-FL I541P

    Plasmid

    #61381

    Purpose

    Expresses Human full length RIPK1 with I541P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I541P mutation (RIPK1 Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I541 to P

    Promoter

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pSicoR-ATF7IPi1

    Plasmid

    #59614

    Purpose

    Expression of shRNA against human ATF7IP

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    ATF7IP (ATF7IP Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pBABEpuro-ARAF_D429A

    Plasmid

    #51080

    Purpose

    mammalian expression of ARAF D429A

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D429A

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 1287 p3E-mut let-7 etv2 3’ UTR

    Plasmid

    #49011

    Purpose

    Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    ets variant gene 2 (etsrp Zebrafish)
    

    Use

    Gateway 3' entry

    Tags

    Expression

    Mutation

    Mutated the Let7 miRNA binding sites

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pDM10

    Plasmid

    #41759

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc Fly, E. coli)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S A378C C451S

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDM8

    Plasmid

    #41758

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc Fly, E. coli)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S S364C C451S

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOU1

    Plasmid

    #41753

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc Fly, E. coli)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C338S C451S

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRS6E1b-luc(-732/-721) mutant1

    Plasmid

    #45384

    Depositor

    Thomas Gelehrter

    Article

    Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

    Insert

    6 copies of hPAI1 promoter -732/-721 mutated at -732/-731
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    mutated -732/-731 from ag to tc

    Promoter

    Available since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pTH443-SUP45-G180A

    Plasmid

    #29386

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G180A mutant gene including genomic sequence 1432…

    Promoter

    Available since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pTH398-SUP45-G54D

    Plasmid

    #29376

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G54D mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pTH372-SUP45-K60A

    Plasmid

    #29379

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K60A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

37. pTH394-SUP45-L34S

    Plasmid

    #29373

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L34S mutant gene (corresponding to the sup45-36ts…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

38. pTH355-SUP45-T55A

    Plasmid

    #29377

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T55A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pTH373-SUP45-S61A

    Plasmid

    #29380

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S61A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pTH401-SUP45-R65C

    Plasmid

    #29381

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R65C mutant gene (corresponding to the sup45-3 al…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only