We narrowed to 26,822 results for: STI

1. pRGE32

   Plasmid

   #63159

   Purpose

   Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

   Depositor

   Yinong Yang

   Article

   Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   Rice snoRNA U3 promoter for gRNA/PTG expression a…

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAG-ERN

   Plasmid

   #80476

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Expression

   Mammalian

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. L4440_BioBrick-hif-1-sgRNA-A

   Plasmid

   #177784

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Expression

   Bacterial

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.tgoGAP1

   Plasmid

   #78737

   Purpose

   Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   tgoGAP1
   

   Tags

   N terminal 81 amino acids of human galactosyltran…

   Expression

   Mammalian

   Mutation

   D117A/D119A/D163A in aequorin moiety

   Promoter

   CMV

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. PTP1B human

   Plasmid

   #102719

   Purpose

   human PTP1B catalytic domain (residues 1-301) in RP1B vector

   Depositor

   Wolfgang Peti

   Article

   Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5.

   Insert

   Protein Tyrosine Phosphatase 1B (PTP1B) (PTPN1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP

   Plasmid

   #234881

   Purpose

   L1HS-silencing plasmid (CRISPRi gRNA2)

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   LacZ gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac(His10-Lckg2a)

   Plasmid

   #177891

   Purpose

   Baculo expression of His10 tag fused to human LckG2A

   Depositor

   Ron Vale

   Article

   Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

   Insert

   Lck (LCK Human)
   

   Tags

   10xHis-TEV

   Expression

   Insect

   Mutation

   G2A

   Promoter

   Polyhedrin

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-X1-Neo-GFP-FAM134B-LIRmut

   Plasmid

   #109027

   Purpose

   stable lentiviral expression of cDNA

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   FAM134B-LIRmut (RETREG1 Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   DDFELL to DDAEAL aa 453-458 to interrupt FAM134B …

   Promoter

   EIF-1a promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-CLIP-PD-L2

    Plasmid

    #223587

    Purpose

    Lentiviral expression of human PD-L2 with CLIP tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    PD-L2 (PDCD1LG2 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    CLIP

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

    Plasmid

    #158963

    Purpose

    Cre-dependet Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Kesavan et al Development. 2020 Jun 8;147(11). pii: de

    Inserts

    hsp70l promoter
    floxed Non-FP cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER-HA-mNG21-10

    Plasmid

    #141165

    Purpose

    Split Fluorescent Reporter for ER Retrograde Trafficking

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    ER-HA-mNG21-10-KDEL
    

    Use

    Lentiviral

    Tags

    Signal Sequence, KDEL and HA

    Expression

    Mammalian

    Mutation

    K128M, S142T, R150M, G172V and K213M

    Promoter

    Ef1a

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p663-UBC-TurboID-V5-CDK13_IDG-K

    Plasmid

    #135267

    Purpose

    Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-CDK13 (CDK13 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cerulean-2xFv-hRIPK3

    Plasmid

    #106079

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    Cerulean-2xFv-hRIPK3 (RIPK3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pRECMYC-Ebu1

    Plasmid

    #240713

    Purpose

    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

    Depositor

    Seth Shipman

    Article

    González-Delgado et al bioRxiv 2025.06.16.660010

    Insert

    Ebu1 RT, Eco1 ncRNA, MspRecT
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR043_Lenti-U6-sgEGFR-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180267

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with EGFR targeting sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    EGFR sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. L4440_BioBrick-hif-1-sgRNA-B

    Plasmid

    #177785

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    hif-1 (hif-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-SYP-HRP

    Plasmid

    #117185

    Purpose

    Plasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSLIK CA Rac1

    Plasmid

    #84605

    Purpose

    Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Constitutively Active, Q61L

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Tags

    Antares

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pEND-446_Cacnes_ProA

    Plasmid

    #225609

    Purpose

    Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-mFlagEts1

    Plasmid

    #86099

    Purpose

    expresses flag tagged Ets1 in mammalian cells

    Depositor

    Barbara Graves

    Article

    Seidel et al Genes Dev. 2002 Jan 1;16(1):127-37.

    Insert

    Ets1 (Ets1 Mouse)
    

    Tags

    Flag tag; kanamycin selection

    Expression

    Mammalian

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEGFP Cep164 (Nigg CW324)

    Plasmid

    #41149

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

    Insert

    Cep164 (CEP164 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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28. p3xFLAG-MKL1

    Plasmid

    #11978

    Purpose

    For mammalian expression of human MKL1 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N579S T716A

    Promoter

    CMV

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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29. hSirt3-RFP

    Plasmid

    #216806

    Purpose

    Human Sirt3-RFP driven by the CaMKII promoter

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Lentiviral

    Tags

    mRFP1

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTrex-Neo-tdTomato

    Plasmid

    #47975

    Purpose

    Trasgenically expresses tandem tdtomato fluorescent protein in T. cruzi

    Depositor

    Rick Tarleton

    Article

    Canavaci et al PLoS Negl Trop Dis. 2010 Jul 13;4(7):e74

    Insert

    tdTomato
    

    Use

    Trypanasoma cruzi expression

    Promoter

    T. cruzi rRNA

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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31. NET31 mRFP-N2 (490)

    Plasmid

    #61984

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

    Insert

    NET31 (TMEM209 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.2/NS1

    Plasmid

    #120824

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS1 ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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33. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CFP_Gem_pcDNA4_HisMaxC

    Plasmid

    #41653

    Depositor

    Henry Colecraft

    Article

    Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

    Insert

    Gem, GTP binding proteins (RGK) (GEM Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Expression

    Bacterial

    Promoter

    BBa_J23118 - Strong Constitutive

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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36. pRDA_722

    Plasmid

    #216077

    Purpose

    CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Destination

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3 GATA1

    Plasmid

    #85693

    Purpose

    Mammalian expression of mouse GATA1

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE

    Plasmid

    #106303

    Purpose

    The plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imaging

    Depositor

    James Zhe Liu

    Article

    Liu et al Proc Natl Acad Sci U S A. 2017 Dec 28. p

    Insert

    scFv-GCN4-HaloTag-GB1-NLS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA3.1-nv5-ER beta

    Plasmid

    #22770

    Depositor

    Donald McDonnell

    Article

    Wittmann et al Cancer Res. 2007 Oct 1. 67(19):9549-60.

    Insert

    estrogen receptor beta (ESR2 Human)
    

    Tags

    v5

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAC-85b

    Plasmid

    #53282

    Purpose

    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, crtY, crtI
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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