8,885 results

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M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-FoxG1

Plasmid

#35547

Depositor

Marius Wernig

Article

Lujan et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

FoxG1 (Foxg1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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CAG-mBrn2

Plasmid

#19711

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

mouse Brn2 (Pou3f2 Mouse)

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pRR-PR-5Z

Plasmid

#23057

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

PR (PGR Human)

Expression

Yeast

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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Clover-mRuby2-FRET-10

Plasmid

#58169

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

Clover and mRuby2

Expression

Mammalian

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMA2641

Plasmid

#25435

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

reverse tetracycline-regulated transactivator advanced

Use

Retroviral

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

Plasmid

#64220

Purpose

Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRc/RSV Flag MKK3(glu)

Plasmid

#14670

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK3(glu) (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-189Glu Thr-193Glu

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D837A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pET15b-Casp8 delta DED

Plasmid

#11827

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain (CASP8 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216)

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pLPC-N MYC

Plasmid

#12540

Depositor

Titia de Lange

Insert

N MYC

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER retro puro Scr shRNA

Plasmid

#30520

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

Scr shRNA

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-TAP

Plasmid

#12570

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Type

Empty backbone

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-mCherry-LacR-BRCA1

Plasmid

#71115

Purpose

mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-link-tdTomato-SpHis5

Plasmid

#44640

Purpose

Yeast tagging vector to create gene fusion with tdTomato with SpHis5 selection

Depositor

Kurt Thorn

Insert

tdTomato

Expression

Yeast

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T2-14-3-3 tau GST

Plasmid

#13281

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 tao (YWHAQ Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP-mCep290

Plasmid

#27379

Depositor

Joseph Gleeson

Article

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Insert

Cep290 (Cep290 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
1271 pBabe puroL Myr HA Akt2

Plasmid

#9018

Depositor

William Sellers

Insert

Akt2 (AKT2 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Axin2-luciferase

Plasmid

#21275

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 promoter (Axin2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Human, Bacillus phage PBS2, Rat)

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-GATA3

Plasmid

#1332

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
syntaxin3-myc-myc-His

Plasmid

#12372

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-p85alpha-Flag

Plasmid

#1399

Depositor

Lewis Cantley

Insert

PI3K regulatory subunit p85alpha (Pik3r1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-zeo smallT

Plasmid

#8583

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-eGFP-LIC

Plasmid

#40768

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Type

Empty backbone

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
WGA-D

Plasmid

#17989

Depositor

Natasha Raikhel

Article

Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

Insert

wheat germ agglutinin isolectin D

Expression

Bacterial

Mutation

N/A

Available Since

July 10, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag AXL

Plasmid

#20428

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AXL (AXL Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
362 pCS Cherry DEST

Plasmid

#13075

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Cherry

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

Plasmid

#39454

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CD (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5 Smad2-HA

Plasmid

#14930

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (SMAD2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pGL2-Mcl-1

Plasmid

#19132

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisD-PATagRFP

Plasmid

#31945

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

His

Expression

Bacterial

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

araB

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Mfn2(K109A)-16xmyc

Plasmid

#26051

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

16xMyc

Expression

Mammalian

Mutation

K109A GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pN1-Lck-GCaMP3

Plasmid

#26974

Depositor

Baljit Khakh

Article

Shigetomi et al Neuron Glia Biol. 2010 Dec 16:1-9. [Epub

Insert

Lck-GCaMP3

Tags

His and Xpress

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,885 results