We narrowed to 13,228 results for: 109

1. pTRE/SP S245X with 5'UTR

   Plasmid

   #92376

   Purpose

   Inducible expression of S245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTRE/SP M87 S245X

   Plasmid

   #92374

   Purpose

   Inducible expression of M87 S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTRE/SP M1 S245X

   Plasmid

   #92372

   Purpose

   Inducible expression of M1S245X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, S245X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTRE/SP M1 N184X

   Plasmid

   #92371

   Purpose

   Inducible expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   Tet-responsive P tight

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Tag/SP S245X with 5'UTR

   Plasmid

   #92370

   Purpose

   expression of 245X spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S245X

   Promoter

   CMV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-Tag/M87 N184X

   Plasmid

   #92367

   Purpose

   expression of M87 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-460 bp deleted, N184X

   Promoter

   CMV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-Tag/M1 N184X

   Plasmid

   #92365

   Purpose

   expression of M1 N184X spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

   Insert

   spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, N184X

   Promoter

   CMV

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. isoform PKC Epsilon (JCE592)

   Plasmid

   #89776

   Purpose

   yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Article

   Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

   Insert

   PKC Epsilon (Prkce Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. isoform PKC Eta (JCE615)

   Plasmid

   #89774

   Purpose

   yeast expression of mouse PKC Eta with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Article

   Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

   Insert

   PKC Eta (Prkch Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Tag 3B/myc-M87L195V

    Plasmid

    #87721

    Purpose

    Expresses myc-M87L195V spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-Tag/S46X without 5'UTR

    Plasmid

    #87730

    Purpose

    Expresses S46X spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S46X; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Tag/S44L without 5'UTR

    Plasmid

    #87728

    Purpose

    Expresses S44L spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S44L; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-Tag/L195V without 5'UTR

    Plasmid

    #87726

    Purpose

    Expresses L195V spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L195V; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Tag/E112K without 5'UTR

    Plasmid

    #87724

    Purpose

    Expresses E112K spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E112K; 5'UTR deleted (1-190 bp)

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-Tag 3B/myc-M87E112K

    Plasmid

    #87720

    Purpose

    Expresses myc-M87E112K spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GTT-c

    Plasmid

    #81108

    Purpose

    Gene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    yemCFP
    

    Use

    Cre/Lox

    Tags

    yemCFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW301ParsR-Amp30T

    Plasmid

    #78653

    Purpose

    pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pSCKtheoRaj12-o11

    Plasmid

    #69941

    Purpose

    pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    TheoHHAzRaj12,cisRaj11,sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCKtheoRaj12-olsa

    Plasmid

    #69940

    Purpose

    pSTC2,theoHHAzRaj12,cisRaj12-sfGFP, KanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Inserts

    TheoHHAzRaj12,cisRaj12,sfGFP
    theoHHAzRaj12,cisRaj12-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAD-hPLEKHM1

    Plasmid

    #64145

    Purpose

    For yeast two hybrid

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    GAL4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

21. psicheck-2-Tas2r3-mod

    Plasmid

    #48175

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> ACG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    TAS2R3 taste 2 receptor member 3 (TAS2R3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    T/C MUTATION IN ATG OF UORF

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

22. psicheck-2-Phb mod

    Plasmid

    #48163

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Phb (Phb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    A/T mutation in the ATG of the uORF

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

23. psicheck-2-Acsm2 mod

    Plasmid

    #48159

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of Acsm2 (Acsm2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    changed A to T in ATG of uORF

    Promoter

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

24. CS2-mCHY-dASUN

    Plasmid

    #47042

    Purpose

    Fluorescent reported for dASUN

    Depositor

    Laura Lee

    Article

    Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    mCHY

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

25. TAL3417

    Plasmid

    #47136

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-dcps-right (dcps Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

26. TAL3416

    Plasmid

    #47135

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-dcps-left (dcps Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pGEMT-Map7-1783-end

    Plasmid

    #46071

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1783-end (Map7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEMT-Map7-856-1351

    Plasmid

    #46069

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-856-1351 (Map7 Mouse)
    

    Use

    Unspecified

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TAL3114

    Plasmid

    #41288

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-mdm2-Left (mdm2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

30. TAL3115

    Plasmid

    #41289

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-mdm2-Right (mdm2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

31. KHBD00003

    Plasmid

    #34166

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6272 (Irbp18 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

32. Tpa1(21-644)

    Plasmid

    #23140

    Depositor

    SeWon Suh

    Article

    Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

    Insert

    Tpa1 (TPA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-20

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

33. Tpa1(273-644)

    Plasmid

    #23141

    Depositor

    SeWon Suh

    Article

    Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

    Insert

    Tpa1 C-terminal domain (TPA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-272

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

34. ZE44 myc-Dapper-2 pCS2P+

    Plasmid

    #17085

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dapper-2 (dact2 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

35. pIS57 PGM2 3'UTR mut

    Plasmid

    #14492

    Depositor

    David Bartel

    Article

    Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

    Insert

    PGM2 3'UTR mutant (PGM2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    CATTCC’s were changed to AAGTAC's

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

36. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEntry_HCoV-OC43-nsp2

    Plasmid

    #168948

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-nsp2 (ORF1ab )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-dCasRx-ALKBH5-PURO

    Plasmid

    #175582

    Purpose

    Expresses dCasRx-ALKBH5 in mammalian cell

    Depositor

    Qi Xie

    Article

    Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

    Insert

    dCasRx-ALKBH5
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    RfxCas13d R239A/H244A/R858A/H863A

    Promoter

    pMSCV

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 myc-RNF114

    Plasmid

    #58295

    Purpose

    mammalian expression of myc tagged RNF114

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pLenti4-Puro-H19

    Plasmid

    #200835

    Purpose

    Lentiviral vector expressing human long non-coding RNA H19

    Depositor

    Reinier Boon

    Article

    Hofmann et al Cardiovasc Res. 2019 Jan 1;115(1):230-24

    Insert

    Human long non-coding RNA H19
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

    Plasmid

    #85583

    Purpose

    cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    Prab-3-GAL4-SK(DBD)-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Prab-3-GAL4-SK(DBD)-VP64

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pNPK-NLS-Vika

    Plasmid

    #79969

    Purpose

    expresses human codon-optimized Vika-recombinase in mammalian cells

    Depositor

    Frank Buchholz

    Article

    Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

    Insert

    human codon-optimized Vika sequence, NLS tagged
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. psPAX2-D64V-NC-MS2

    Plasmid

    #122944

    Purpose

    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    NC-MCP (MS2 coat protein) fusion protein in Gag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. psfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164477

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. PRE-Hsp70BbCas9_1.3

    Plasmid

    #190797

    Purpose

    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLVX-opto-mMLKL

    Plasmid

    #208784

    Purpose

    Inducibly expresses mouse MLKL fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mMLKL Cry2 (Mlkl Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pDule2-pCNF

    Plasmid

    #85495

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. WT GBA pcDNA3.1

    Plasmid

    #188580

    Purpose

    Ectopic expression of wild-type GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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