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Showing: 1001 - 1050 of 6893 results

  8. pLenti-EF1a-MTS-FLAG-LOV*

    Plasmid
    #202208
    Purpose
    Plasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells
    Depositor
    Thomas Muir
    Article
    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
    Use
    Lentiviral
    Tags
    FLAG and LOV*
    Expression
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    202208-plasmid-map-sequence-id-402886

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  9. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid
    #192585
    Purpose
    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    Lentiviral
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-alpha
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192585-plasmid-map-sequence-id-379281

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  30. KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

    Plasmid
    #121806
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + EF1a::TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121806-plasmid-map-sequence-id-237315

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  31. pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

    Plasmid
    #69586
    Purpose
    Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus
    Depositor
    Galit Lahav
    Article
    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:
    Insert
    p53-L344P (TP53 Human)
    Use
    Lentiviral
    Tags
    mKate2 and split mVenus - N term
    Expression
    Mammalian
    Mutation
    p53 L344P mutation that prevents dimerization and…
    Promoter
    EF1alpha
    Available since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    69586-plasmid-map-sequence-id-135228

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  34. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

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  35. pUC-hU6-crBACH1-array_EF1a-BFP

    Plasmid
    #224787
    Purpose
    BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    crBACH1-1, crBACH1-2, crBACH1-3,
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6 and hU6-2xTetO
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224787-plasmid-map-sequence-id-450827

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  47. pAAV-Ef1a-FAS-GCaMP6f-WPRE

    Plasmid
    #141237
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    GCaMP6f
    Use
    AAV and Cre/Lox
    Tags
    6XHIS and Xpress
    Expression
    Mutation
    Promoter
    Human elongation factor-1 alpha (EF-1 alpha)
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    141237-plasmid-map-sequence-id-431695

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  50. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Luke Gilbert
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Ef1alpha
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217304-plasmid-map-sequence-id-429717

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Showing: 1001 - 1050 of 6893 results