We narrowed to 7,333 results for: ef1a
-
Plasmid#137124PurposeIntersectional viral expression of GCaMP6F in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6F
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-ConVERGD-eGFP-WPRE
Plasmid#218745PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVPromoterEf1aAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP
Plasmid#155307PurposeRfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFPDepositorInsertRfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
UseLentiviralExpressionMammalianPromoterhU6, EF1aAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-WPRE
Plasmid#179460PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV EF1a DIO iChloC 2A dsRed
Plasmid#70762PurposeAn improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVExpressionMammalianMutationE83Q,E90R,E101S,D156N,T159CPromoterEF1aAvailable SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-EYFP
Plasmid#231927PurposeCre-off/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVPromoterEf1aAvailable SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald D40G
Plasmid#164211PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE
Plasmid#202608PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P-Kv
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1aL-Cre-IRES-NeoR-W
Plasmid#126700Purposelentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassetteDepositorInsertCre-IRES
UseCre/Lox and LentiviralTagsSV40 NLSPromoterEf1aAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro
Plasmid#196196Purposehomologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
UseHomology repair donorTagsV5-APEX2-mScarlet-IAvailable SinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTS1-IRES-Puromycin
Plasmid#134665PurposeExpresses EGFP-tagged peroxisome markerDepositorInserttripeptide SKL (Peroxisome targeting signal 1)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-EGFP-WPRE-pA
Plasmid#37084PurposeCre-dependent expression of EGFPDepositorInsertEGFP
UseAAV and Cre/Lox; Cre-onPromoterEF1aAvailable SinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPPromoterEF1aAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R346C
Plasmid#164213PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald R235Q
Plasmid#164212PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoterDepositorAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-ngRNA+41_EF1a-puroR
Plasmid#207358PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 +41 ngRNA
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC374 - pAAV EF1a DIO iRFP
Plasmid#47626PurposeAn AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorInsertInfrared fluorescent protein
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11
Plasmid#193752Purposehuman codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGHDepositorInsertEF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA
TagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-EGFP-WPRE
Plasmid#187103PurposeDouble floxed EGFP under the control of EF1a promoterDepositorInsertEGFP
UseAAVPromoterEF1aAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE
Plasmid#179459PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P-Kv
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-tdTomato-WPRE-pGHpA
Plasmid#67527Purpose"Expresses the fluorescent protein tdTomato"DepositorInserttandem dimer tomato
UseAAVExpressionMammalianPromoterEF1aAvailable SinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ngn3ER-PuroR-mCherry
Plasmid#197385PurposeTo establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)DepositorInsertNgn3ER-2A-PuroR-2A-mCherry
UseLentiviralExpressionMammalianPromoterEF1AAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-GCaMP6s-WPRE-pGHpA
Plasmid#67526Purpose"Fluorescent reporter for calcium imaging"DepositorInsertGCaMP6s
UseAAVTags6xHis, T7 tag, and Xpress epitopeExpressionMammalianPromoterEF1aAvailable SinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-N2c_envA-IRES-Neo
Plasmid#172368PurposeGeneration of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)DepositorInsertenvA + Neo
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
Plasmid#211466PurposeLentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signalDepositorInsertmito-mCherry-GFP1-10
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PG13-mCherry-EF1a-PuroR-EGFP
Plasmid#195292PurposepiggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)DepositorInsertsmCherry
PuroR-P2A-EGFP
UsePiggybac transposonExpressionMammalianAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2
Plasmid#170823PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA2
UsePiggybac transposonExpressionMammalianPromoterU6Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0045-EF1a-PguCas13b-NES-HIV
Plasmid#103861PurposeExpresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertPguCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-ChrimsonR-tdTomato
Plasmid#171027PurposeExpresses ChrimsonR-tdTomato fusion after Flp-dependent recombinationDepositorHas ServiceAAV1 and AAV9InsertChrimsonR-tdTomato
UseAAVTagsChrimsonR is fused to tdTomatoPromoterEf1aAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mRuby3-2A-Cre
Plasmid#203844PurposeFlp-dependent Cre and mRuby3 expressionDepositorInsertCre
UseAAVTagsmRuby3-2AAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
plenti2-EF1a-ChR2-EYFP-WRPE
Plasmid#125255Purpose3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInserthChR2
UseLentiviralTagsEYFPExpressionMammalianAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-OCT4
Plasmid#130692PurposeLentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.DepositorInserthuman OCT4 (POU5F1 Human)
UseLentiviralAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-FKRP-EF1a-BSD
Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-FLEx-ASAP4b-Kv-WPRE
Plasmid#201032PurposeExpresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent mannerDepositorInsertASAP4e-Kv
UseAAVAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#133786PurposeCre-dependent tdTomato fluorescent reporter for cell labelingDepositorInsertpAAV-EF1a-DIO-tdTomato-WPRE
UseAAV and Cre/LoxExpressionMammalianAvailable SinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode
Plasmid#170236PurposeBarcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-oG-WPRE-hGH
Plasmid#74290PurposepAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterEf1aAvailable SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC150-PBLHL-4xHS-EF1a-DEST
Plasmid#48234PurposePiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection markerDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterEF1aAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
Plasmid#35498PurposeAAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorInsertChR1-VChR1 Chimera
UseAAVTagsmCherryExpressionMammalianMutationE122T and E162TPromoterEf1aAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-TAL1-IRES-Puro
Plasmid#61065Purposelentiviral vector for TAL1DepositorAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVH-EF1a-GFP-P2A-SOX2
Plasmid#130693PurposeLentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.DepositorInserthuman SOX2 (SOX2 Human)
UseLentiviralAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE
Plasmid#198515PurposeExpresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianPromoterEF-1aAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a-MCS-WPRE-SV40 - GCH13
Plasmid#230820PurposeAAV cloning vector for expression under the EF1a promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP469 pEF1a-mCherry-EMPTY-gRNA
Plasmid#100958PurposemCherry driven by EF1a, empty gRNA scaffold driven by U6DepositorInsertgRNA empty backbone with RFP
UseCRISPRTagsRFPExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Flex-Axon-GCaMP7b
Plasmid#135419PurposeCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.DepositorInsertaxon-jGCaMP7b
UseAAVTags6xHis and GAP43 palmitoylation domainExpressionMammalianPromoterEf1aAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0044-EF1a-RanCas13b-NES-mapk
Plasmid#103855PurposeExpresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasmDepositorInsertRanCas13b
UseCRISPR and LentiviralTags3xHA and mapk NESExpressionMammalianAvailable SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-CAG-hMGT-Ef1a-puro
Plasmid#172395PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-mCherry-RAB7
Plasmid#221555PurposeExpresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a SS18-Frb PGK Puro
Plasmid#102811PurposeLentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion proteinDepositorInsertSS18-Frb
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only