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We narrowed to 10,763 results for: ESP

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Showing: 10451 - 10500 of 10763 results

  1. YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228883
    Purpose
    PiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228883-plasmid-map-sequence-id-454808

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  2. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  8. pDYT001

    Plasmid
    #186549
    Purpose
    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)
    Depositor
    Jonathan Weissman
    Article
    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00
    Insert
    14-bp random integration barcode and three target sites and 3x sgRNAs
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Aug. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186549-plasmid-map-sequence-id-402892

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  10. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available Since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

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  13. pGF1-4eCOL2A1

    Plasmid
    #97210
    Purpose
    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97210-plasmid-map-sequence-id-182599

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  21. ORAI1-YFP T184M

    Plasmid
    #114181
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP T184M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    T184M
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114181-plasmid-map-sequence-id-218676

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  22. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  23. pUC57-4eCOL2A1

    Plasmid
    #97211
    Purpose
    For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors
    Depositor
    Ryan Porter
    Article
    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d
    Insert
    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    Use
    Vector is designed for cloning and generation of …
    Promoter
    COL2A1 regulatory elements
    Available Since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    97211-plasmid-map-sequence-id-181460

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  24. pSEVA228-pro4IUPi

    Plasmid
    #122018
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pro4
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122018-plasmid-map-sequence-id-238966

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  30. ORAI1-YFP V107M

    Plasmid
    #114180
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Article
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Insert
    ORAI1-YFP V107M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114180-plasmid-map-sequence-id-218674

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  32. pAR100

    Plasmid
    #180377
    Purpose
    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).
    Depositor
    Alec Redwood
    Article
    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:
    Inserts
    NFAT-Luciferase
    hCD8A
    Expression
    Bacterial and Mammalian
    Promoter
    hEF1-HTLV and minimal promoter and an NFAT respon…
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    180377-plasmid-map-sequence-id-370772

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  33. pBS-AMVpl-δWT-nAchR

    Plasmid
    #97188
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Delta nicotinic acetylcholine receptor (Chrnd Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    97188-plasmid-map-sequence-id-191155

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  35. pBS-AMVpl-γWT-nAchR

    Plasmid
    #97187
    Purpose
    To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
    Depositor
    Henry Lester
    Article
    Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.
    Insert
    Gamma nicotinic acetylcholine receptor (Chrng Mouse)
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97187-plasmid-map-sequence-id-183032

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  37. pOUPc-UL148-mCherry

    Plasmid
    #179279
    Purpose
    All in one "tet-on" lentivirus vector that expresses human cytomegalovirus UL148 fused to an mCherry tag at its cytoplasmic tail. UL148 reorganizes the ER and activates the UPR.
    Depositor
    Jeremy Kamil
    Article
    Zhang et al mBio. 2019 Dec 10;10(6). pii: mBio.02110
    Insert
    UL148 fused to mCherry
    Use
    Lentiviral; Tet-on, all-in-one lentiviral vector
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Tet responsive element 3G
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    179279-plasmid-map-sequence-id-355068

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  44. pLCHKO_GFP_Luciferase

    Plasmid
    #155079
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Aug. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    155079-plasmid-map-sequence-id-308671

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  46. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

    Plasmid
    #46941
    Purpose
    Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with TALEN TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded TALEN TS from 163-218, truncated 25 amin…
    Promoter
    SFFV
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    46941-plasmid-map-sequence-id-67512

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Showing: 10451 - 10500 of 10763 results