We narrowed to 22,342 results for: Sis

1. pGWB520

   Plasmid

   #74862

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-AktAR-Kras

   Plasmid

   #123357

   Purpose

   FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR-Kras
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMpGE006

   Plasmid

   #108722

   Purpose

   Cas9 expression in M.polymorpha

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Insert

   Atco-Cas9-Pea3ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MpEFpro

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDUP1

   Plasmid

   #223516

   Purpose

   inactive cogfp under control of Ptet, cogfp under control of Ptac

   Depositor

   Yolanda Schaerli

   Article

   Ljiljana et al eLife13:RP97216

   Inserts

   CoGFP
   CoGFP inactivated
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   anti-dimerization mutations S129G, 22 C154S, D156…

   Promoter

   Ptac, Ptac and Ptet, and Ptet

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RC(WW)-LgBit

   Plasmid

   #208931

   Purpose

   Recognition domain for in vitro MAPK-activity sensor

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   WW domain of PIN1 (PIN1 Human)
   

   Use

   Tags

   HISx6, LgBit, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS1422

   Plasmid

   #12548

   Depositor

   Robert Sobol

   Article

   Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

   Insert

   human Methylpurine DNA glycosylase (MPG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCK074 p5E exorh:EGFP

   Plasmid

   #195951

   Purpose

   Gateway p5E vector with reporter

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:EGFP
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE657

   Plasmid

   #153255

   Purpose

   M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB420

    Plasmid

    #74814

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPRai

    Plasmid

    #189938

    Purpose

    Expresses CRISPRai cassette under EF1a promoter

    Depositor

    Yu-Chen Hu

    Article

    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

    Insert

    CRISPRai cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer-rat EF1 alpha

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

    Plasmid

    #115487

    Purpose

    CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. k3(long) luciferase

    Plasmid

    #11110

    Depositor

    Anjana Rao

    Article

    Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

    Insert

    k3(L) (TNF Human)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

14. drebrin-His

    Plasmid

    #40363

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pFnCpf1GG

    Plasmid

    #131010

    Purpose

    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of FnCas12a
    GFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-FLAG-CyclinF-ΔF

    Plasmid

    #32973

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F-box mutations: L35A and P36A

    Promoter

    CMV

    Available since

    Feb. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pLVH-EF1A-BRD3R

    Plasmid

    #130696

    Purpose

    Lentiviral plasmids encoding human BRD3R

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    Human BRD3R (BRD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO HSPA2

    Plasmid

    #19458

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA2 (HSPA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA6-LRRC26-V5-His

    Plasmid

    #127817

    Purpose

    Expression of human LRRC26 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC26 (LRRC26 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mRIP3 GFP RHIM mut

    Plasmid

    #41384

    Depositor

    Francis Chan

    Article

    Cho et al Cell. 2009 Jun 12;137(6):1112-23.

    Insert

    RIP3 (Ripk3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RHIM mutant

    Promoter

    CMV

    Available since

    Dec. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONOR-tagBFP-PSM-EGFP

    Plasmid

    #100603

    Purpose

    Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    EGFP-T2A-Puro and tagBFP

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

    Plasmid

    #200014

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEG302 22aa SunTag VP64 g4+g17

    Plasmid

    #120252

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEYFP-Pds1(434)

    Plasmid

    #39847

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MSCV eIF4E S209D IRES GFP

    Plasmid

    #18765

    Depositor

    Scott Lowe

    Article

    Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

    Insert

    eIF4E S209D (Eif4e Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    S209D

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pJMP1360

    Plasmid

    #119278

    Purpose

    for cloning new sgRNAs

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO GFP DNAJB8

    Plasmid

    #19504

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB8 (DNAJB8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi, Ikuko Hara-Nishimura

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVG1

    Plasmid

    #111444

    Purpose

    Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGWB508

    Plasmid

    #74850

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDH-CAG-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160500

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Tom20-mCherry-GAI(1-92)

    Plasmid

    #37316

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Use

    Tags

    Tom20 and mCherry

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pm

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJMP1189

    Plasmid

    #119257

    Purpose

    rfp "test" strain

    Depositor

    Jason Peters, Oren Rosenberg, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCB-CBEv2

    Plasmid

    #221139

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. CLPIT Cry2PHR-mCh-RhoA

    Plasmid

    #42959

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Off promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1(+)-mTagBFP2-MRTF-B

    Plasmid

    #182062

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-B (Mrtfb Mouse)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDGE479

    Plasmid

    #153270

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRetroSuper-shFyn

    Plasmid

    #26985

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Fyn (FYN Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

41. pNoTag-DGCR8

    Plasmid

    #164694

    Purpose

    Express DGCR8 without tag in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCS2+PIF6CreN

    Plasmid

    #131780

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PIF6CreN
    

    Use

    Cre/Lox; Zebrafish expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGWB450

    Plasmid

    #74832

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pFB-GTE-COI1

    Plasmid

    #29516

    Depositor

    Ning Zheng

    Article

    Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

    Insert

    COI1 (COI1 Mustard Weed)
    

    Use

    Tags

    GST and TEV cleavage site

    Expression

    Insect

    Mutation

    Note that the first AA in TEV site is R, not E

    Promoter

    Available since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

45. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

    Plasmid

    #194519

    Purpose

    Dual fluorescence reporter for studying protein degradation

    Depositor

    Tommer Ravid

    Article

    Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

    Insert

    TIF4631 leader sequence
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. UKNP6.1.1

    Plasmid

    #98386

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194385

    Purpose

    Gateway Destination vector for split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-NTEV-TCS-GV-2xHA
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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49. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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50. pHelper-CBE-sgRNA

    Plasmid

    #221137

    Purpose

    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with Cas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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