Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

159,557 results

Showing: 4241 - 4260 of 159557 results

  1. pPBbleo-EGF-ScNeo

    Plasmid
    #209902
    Purpose
    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    EGF-ScNeo (EGF )
    Use
    Tags
    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209902-plasmid-map-sequence-id-426731

    Enlarge

  8. pDRF1 Cyterm-ymStayGold

    Plasmid
    #219855
    Purpose
    expression of Cyterm-ymStayGoldd in budding yeast
    Depositor
    Bas Teusink
    Insert
    Cyterm-ymStayGold
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219855-plasmid-map-sequence-id-437577

    Enlarge

  9. pBABE 3xHA-MKK6-EE puro

    Plasmid
    #216791
    Purpose
    Mammalian expression of constitutively active human MKK6
    Depositor
    Kevin Janes
    Insert
    MKK6 (MAP2K6 Human)
    Use
    Retroviral
    Tags
    3x-HA
    Expression
    Mammalian
    Mutation
    S207E and T211E, constitutively active
    Promoter
    LTR
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216791-plasmid-map-sequence-id-434910

    Enlarge

  11. UTS2R-DuET

    Plasmid
    #213386
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    UTS2R (UTS2R Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213386-plasmid-map-sequence-id-430893

    Enlarge

  18. TFORF2053

    Plasmid
    #142912
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    BCL6 (BCL6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142912-plasmid-map-sequence-id-391564

    Enlarge

  19. pET-EIN-FTO(32-505)

    Plasmid
    #186466
    Purpose
    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
    Depositor
    Vincenzo Venditti
    Article
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    Fat mass obesity associated protein (FTO Human)
    Use
    Tags
    6 Histidine-EIN (N-terminal domain of E.coli Enzy…
    Expression
    Bacterial
    Mutation
    amino acid 32-505
    Promoter
    T7
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186466-plasmid-map-sequence-id-365741

    Enlarge

Showing: 4241 - 4260 of 159557 results