We narrowed to 31,552 results for: Eng;

1. pYPQ141B-ge4.1

   Plasmid

   #205144

   Purpose

   Guide RNA expression cassette harboring AtU3 promoter, ge4.1 5’ modification, and ge4.1 3’ modification

   Depositor

   Yiping Qi

   Article

   Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU3

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYPQ133-tRNA

   Plasmid

   #179212

   Purpose

   Golden Gate entry vector; 3rd gRNA with tRNA

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   no promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR1a-MTS-jAspSnFR3

   Plasmid

   #203461

   Purpose

   Mitochondrial directed jAspSnFR3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3
   

   Use

   Gateway entry vector

   Tags

   Mitochondrial localization signal

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VV063: 1xCox8 - superecliptic pHluorin in fck

   Plasmid

   #58500

   Purpose

   Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - Superecliptic pHluorin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SCP1-CMVenh-luc

   Plasmid

   #22216

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-CMVenh luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK DPC4 Flag (aka: Smad4 Flag)

   Plasmid

   #12627

   Depositor

   Rik Derynck

   Article

   Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

   Insert

   DPC4 (SMAD4 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pSB1C3-J23110-B0034-mRFP1E

   Plasmid

   #160442

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

   Plasmid

   #61255

   Purpose

   CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   2xTY1-V5
   

   Use

   CRISPR

   Tags

   TY1 and V5

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFtnA-Halo

   Plasmid

   #98281

   Purpose

   mammalian expression of FtnA-Halo

   Depositor

   Mike Heilemann

   Article

   Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

   Insert

   FtnA
   

   Use

   Tags

   Halo-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT/TO_NES_HaloTag7

    Plasmid

    #175527

    Purpose

    Strict cytosolic expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BSP610

    Plasmid

    #122255

    Purpose

    expression vector for mtagBFP2 under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagBFP2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGT-Ah9

    Plasmid

    #122576

    Purpose

    MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    tetQ/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAL-pck_NT

    Plasmid

    #74069

    Purpose

    pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (NT)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pENTR APP shRNA 1

    Plasmid

    #30134

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP shRNA 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pJG/ Inducible ALPHA MHC

    Plasmid

    #55792

    Purpose

    transgene expression driven by inducible ALPHA MHC promoter

    Depositor

    Jeffrey Robbins

    Article

    Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Inducible ALPHA MHC

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. TfRΔEctoR1-mRFP-GFPnb

    Plasmid

    #113555

    Purpose

    Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

    Depositor

    Jeanne Stachowiak

    Article

    DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

    Insert

    TfRΔEctoR1-mRFP-GFPnb
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EphA1-RTK-Cry2olig

    Plasmid

    #176601

    Purpose

    EphrinA1 RTK fusion to Cry2olig - RTK domain only

    Depositor

    Robert Hughes

    Article

    Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

    Insert

    EphA1rtk-Cry2olig-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGlnA-Ypet

    Plasmid

    #98278

    Purpose

    mammalian expression of GlnA-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    GlnA
    

    Use

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO_LAMP1_HaloTag7

    Plasmid

    #175531

    Purpose

    Lyzosomal expression of HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEntr_PE2

    Plasmid

    #149548

    Purpose

    Gateway entry clone with PE2 enzyme (with stop codon)

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR; Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3xP3-EGFP.vas-int.NLS

    Plasmid

    #60948

    Purpose

    Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    vas-phiC31 integrase.NLS
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    vasa

    Available since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

22. VV064: 1xCox8 - ratiometric pHluorin in fck

    Plasmid

    #58502

    Purpose

    Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUSE-RapR-Src-myc

    Plasmid

    #25933

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-Src (Src Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCellFree_G03 PC

    Plasmid

    #67132

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PC (PC Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Act5c

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pREPK219H

    Plasmid

    #51492

    Purpose

    pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Rep/RepA
    

    Use

    Plant expression t-dna

    Tags

    Expression

    Mutation

    changed lysine 219 to histidine

    Promoter

    2x35S

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMTU-DO-URA-C4

    Plasmid

    #160339

    Purpose

    High-copy expression vector for K. marxianus, uracil selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BSP605

    Plasmid

    #122250

    Purpose

    expression vector for mCerulean under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mCerulean
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET51b_SNAP-ecDHFR

    Plasmid

    #187108

    Purpose

    Expression of SNAP-ecDHFR fusion in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    SNAP-ecDHFR
    

    Use

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYPQ134-tRNA

    Plasmid

    #179213

    Purpose

    Golden Gate entry vector; 4th gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pWS2247

    Plasmid

    #123026

    Purpose

    Tet promoter (TetO(x7)-pLEU2m)

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    pTet [2]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAM282

    Plasmid

    #226121

    Purpose

    Expression of cdc48

    Depositor

    Andreas Martin

    Article

    Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

    Insert

    His6-3C-Cdc48 (CDC48 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET22-HT/spoR

    Plasmid

    #73631

    Purpose

    Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    guanylate kinase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCM291rfp

    Plasmid

    #59775

    Purpose

    pCMrfp with TrfA mutation (291E to G)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP

    Plasmid

    #162233

    Purpose

    Lentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    anti-GFP nanobody lag16lag2 and IFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSMVP-Cas9N

    Plasmid

    #80934

    Purpose

    Plasmid that expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LP538

    Plasmid

    #90202

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC8 full length

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC8 (TRAPPC8 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-51b-HaloTag10

    Plasmid

    #175520

    Purpose

    Expression of HaloTag10 in bacterial cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag10
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    HaloTag10 = HaloTag7-Q165W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGT-Ah5

    Plasmid

    #122572

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSMVP-Cas9C

    Plasmid

    #80939

    Purpose

    Plasmid that expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge