174,131 results

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pCMV5B-Flag-Smurf1 C699A

Plasmid

#11753

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

C699A: catalytically inactive

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV5B-TGFbeta receptor I K232R

Plasmid

#11763

Depositor

Jeff Wrana

Article

Wieser et al EMBO J. 1995 May 15. 14(10):2199-208.

Insert

TGFbeta receptor I (TGFBR1 Human)

Tags

6xHIS

Expression

Mammalian

Mutation

K232R: kinase dead

Promoter

CMV

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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EGFR (del3) L747-E749del, A750P

Plasmid

#11015

Purpose

Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (del3) (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L747-E749 deleted, A750P

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLX_305_Ovalbumin

Plasmid

#184924

Purpose

Lentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

Ovalbumin (OVAL Chicken)

Use

Lentiviral

Expression

Mammalian

Mutation

Our vector has an A188T mutation that does not im…

Promoter

PGK

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHCMV_Bless

Plasmid

#207255

Purpose

Mammalian expression of Baboon Endogenous Retrovirus env protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

BaboonEndogenousRetrovirus_Rless_env

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Article

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 S

Plasmid

#149329

Purpose

Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET263-pUC57 24xMS2V7

Plasmid

#140705

Purpose

MS2V7 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V7

Use

Cloning vector

Mutation

24 MS2 loops V7, high-affinity C-variant, inter-s…

Promoter

None

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

Plasmid

#141369

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEVOL-pylT-N346A/C348A

Plasmid

#127411

Purpose

Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

Insert

Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair

Expression

Bacterial

Mutation

N346A-C348A

Promoter

araBAD

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP mCherry

Plasmid

#122963

Purpose

mCherry-based fluorescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP mCherry

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Article

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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VPS13A^mCherry

Plasmid

#118758

Purpose

VPS13A internally tagged with mCherry at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

mCherry inserted at position 1372

Expression

Mammalian

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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