19,210 results

1. pT2/shp53/GFP4

   Plasmid

   #20208

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   shp53/GFP4
   

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUB_smFLAG_KDM5B_MS2

   Plasmid

   #81084

   Purpose

   Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

   Depositor

   Tim Stasevich

   Article

   Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

   Insert

   smFLAG_KDM5B_24xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUCmini-iCAP-AAV9.452sub.LUNG1

   Plasmid

   #184592

   Purpose

   non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection

   Depositor

   Viviana Gradinaru

   Article

   Goertsen et al Mol Ther Methods Clin Dev. 2022 Aug 1;26

   Insert

   Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   7 amino acid substitution between VP1 452 and VP1…

   Promoter

   p41

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

   Plasmid

   #158967

   Purpose

   Ubiquitous expression of mtON in C. elegans

   Depositor

   Andrew Wojtovich

   Article

   Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

   Insert

   eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC18-mini-Tn7T-Lac-dCas9 (Ptac)

   Plasmid

   #105234

   Purpose

   Tn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Ptac promoter for expression in P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Bacterial

   Mutation

   D10A, H599A

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mbYFPQS

   Plasmid

   #80742

   Purpose

   membrane-targeted biosensor for monitoring changes in intracellular chloride

   Depositor

   Susan Amara, Susan Ingram

   Article

   Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

   Insert

   YFPQS
   

   Use

   Tags

   palmitoylation signal sequence of neuromodulin

   Expression

   Mammalian

   Mutation

   H148Q (CAC to CAG) and V163S (GTG to AGC).

   Promoter

   CMV

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA 3.1(-) mouse C/EBP beta (LAP)

   Plasmid

   #12557

   Depositor

   Peter Johnson

   Insert

   CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs-Stat3-C

   Plasmid

   #13373

   Purpose

   Retroviral expression of dominant active mouse Stat3

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed Alanine 661 to cysteine and asparagine 66…

   Promoter

   Available since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

    Plasmid

    #31357

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    NSD3 (NSD3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pT-CA-GFP-IRES-mLumin

    Plasmid

    #32749

    Depositor

    Jeanne Hardy

    Article

    Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

    Inserts

    CA-GFP
    mLumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S158A and S65T

    Promoter

    CMV

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hsyn-GRAB_Ado1.0mut

    Plasmid

    #153287

    Purpose

    Express the adenosine-insensitive control sensor GRAB_Ado1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine-insensitive sensor GRAB_Ado1.0mut (control sensor)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1-hRabin8 WT

    Plasmid

    #26726

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pRK5 HA-Nprl2

    Plasmid

    #99709

    Purpose

    Mammalian expression of HA-Nprl2

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Insert

    Nprl2 (NPRL2 )
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti.CMV/TO_PRKAA1

    Plasmid

    #74446

    Purpose

    Lentiviral expression vector encoding untagged WT human AMPK alpha 1

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-CHRNA7

    Plasmid

    #62276

    Purpose

    mammalian expression of human Alpha7 (CHRNA7)

    Depositor

    Henry Lester, Sherry Leonard

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRNA7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pZW12 (CK2beta)

    Plasmid

    #27088

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ALKBH5H204A-dCas9

    Plasmid

    #134784

    Purpose

    Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H204A

    Promoter

    CMV

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FRP2350

    Plasmid

    #127580

    Purpose

    Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PtetO7.1_Flagtag_8Glycine linker
    

    Use

    Synthetic Biology; Pcr based tagging

    Tags

    Flag tag, 8glycine linker

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MCP-YFP

    Plasmid

    #101160

    Purpose

    to express MCP-YFP fusion protein

    Depositor

    Mazhar Adli

    Article

    Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

    Insert

    MCP-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CWB-cyan pre-eGRASP(p32)

    Plasmid

    #111579

    Purpose

    An AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    cyan pre-eGRASP(p32)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSV-PKI-v2

    Plasmid

    #45066

    Purpose

    Expression of PKI (PKA inhibitor)

    Depositor

    Richard Maurer

    Insert

    cAMP-dependent protein kinase inhibitor alpha
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST_TAF15

    Plasmid

    #26379

    Depositor

    Thomas Tuschl

    Article

    Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Tags

    FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

25. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA6-N-3XFLAG-Vcp

    Plasmid

    #123600

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    valosin containing protein (Vcp Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mPapaya-pBAD

    Plasmid

    #54838

    Purpose

    Localization: Protein Expression Vector , Excitation: 530, Emission: 541

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPapaya

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EP-Pol vector

    Plasmid

    #131686

    Purpose

    A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

    Depositor

    Matthew D. Shoulders

    Article

    Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

    Insert

    Adenovirus DNA polymerase
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    F421Y, D827A

    Promoter

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-TRE-mRuby2

    Plasmid

    #99114

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1+ SMN1 myc HIS

    Plasmid

    #71687

    Purpose

    Mammalian expression of SMN1

    Depositor

    Gideon Dreyfuss

    Insert

    SMN1 (SMN1 Human)
    

    Use

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRIP3-mCherry

    Plasmid

    #61386

    Purpose

    Expresses RIP3-mCherry in mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    RIP3 (RIPK3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

    Plasmid

    #154088

    Purpose

    Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet on

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOPING

    Plasmid

    #26046

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    SS peptide-LacZ-KHHHHHH
    

    Use

    Tags

    KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-2xPH-TAPP1 WT

    Plasmid

    #161990

    Purpose

    Expresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    PH-TAPP1 (PLEKHA1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TACR1-Tango

    Plasmid

    #66514

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TACR1 (TACR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

37. mCitrine-C1

    Plasmid

    #54587

    Purpose

    Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

    Depositor

    Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Type

    Empty backbone

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. mOrange2-N1

    Plasmid

    #54568

    Purpose

    Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Nathan Shaner, Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Arp3-pmCherryC1

    Plasmid

    #27682

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Arp3 (ACTR3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLenti PGK mKate2-OGT WT (Puro)

    Plasmid

    #154290

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    PGK

    Available since

    Dec. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGL3 1kb prom. CD274

    Plasmid

    #107002

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pBudORF2-CH

    Plasmid

    #51289

    Purpose

    expresses the codon optimized ORF2 of L1RP with his tag

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 ORF2 (L1RE1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pBA409

    Plasmid

    #85971

    Purpose

    EF1a-sfGFP-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sfGFP
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCS2-Jmjd3-F

    Plasmid

    #17440

    Purpose

    Expression of Jmjd3 in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    JMJD3 (Kdm6b Mouse)
    

    Use

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pDSG375

    Plasmid

    #115603

    Purpose

    surface-displayed Nb2; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-Flag-Cortactin

    Plasmid

    #74476

    Purpose

    This plasmid was constructed to study cortactin interactions

    Depositor

    J Thomas Parsons

    Article

    Weed et al J Cell Biol. 2000 Oct 2;151(1):29-40.

    Insert

    Cortactin (Cttn Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Peft-3::cas9-SV40_NLS::tbb-2 3'UTR

    Plasmid

    #46168

    Purpose

    Codon optimized Cas9_SV40 NLS with intron

    Depositor

    John Calarco

    Article

    Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

    Insert

    codon optimized Cas9_SV40 NLS with intron
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    intron inserted at position 3113-3163, and SV40 N…

    Promoter

    eef-1A.1 (eft-3)

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHAGE-mt-mKeima-P2A-FRB-Fis1

    Plasmid

    #135295

    Purpose

    stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

    Promoter

    Available since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. EYFP-hCaM

    Plasmid

    #47603

    Depositor

    Emanuel Strehler

    Article

    Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

    Insert

    CaM (CALM3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge