We narrowed to 15,742 results for: grna

1. pgRNAtet-phaZ

   Plasmid

   #169874

   Purpose

   Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   sgRNA towards phaZ in Pseudomonas putida
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti.Cas9.BFP.sgRNA.parental

   Plasmid

   #196715

   Purpose

   Sortable WT-SpCas9 expression and guide cassette (1-vector system).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

6. py100-sgRNA_hVEGFA

   Plasmid

   #198282

   Purpose

   Expression of SpCas9 guide RNA specific for human VEGFA locus

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   py100-sgRNA_hVEGFA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

   Plasmid

   #136652

   Purpose

   Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

   Inserts

   St1Cas9 LMD-9
   sgRNA for St1Cas9
   APOBEC-1 (APOBEC1 Human)
   UGI
   HA tag
   

   Use

   CRISPR and Synthetic Biology

   Tags

   2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available Since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Construct 11 - U6p_70nt_stgRNA

   Plasmid

   #81250

   Purpose

   Expresses 70nt stgRNA

   Depositor

   Timothy Lu

   Article

   Perli et al Science. 2016 Aug 18. pii: aag0511.

   Insert

   sgRNA
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-th-sgRNA

   Plasmid

   #65565

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish th locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   th sgRNA (th Zebrafish)
   

   Use

   In vitro rna transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCY_gRNA_hPAX7-Pro_C6

    Plasmid

    #160457

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C6
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCY_gRNA_hPAX7-Pro_C5

    Plasmid

    #160456

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCY_gRNA_hPAX7-Pro_C4

    Plasmid

    #160455

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCY_gRNA_hPAX7-Pro_C3

    Plasmid

    #160454

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-U6-AasgRNA

    Plasmid

    #121954

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tubb3gRNA-mCherry

    Plasmid

    #87111

    Purpose

    mouse Tubb3 target gRNA expression plasmid with CAGmCherry

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    Mouse Tubb3 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197979

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLH-stsgRNA2.1

    Plasmid

    #64117

    Purpose

    Vector for expression of St1 sgRNA2.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MS2-pegRNA

    Plasmid

    #181811

    Purpose

    ms2 incorporated pegRNA-1.1

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    MS2-pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pgRNAkan-glpR

    Plasmid

    #169873

    Purpose

    Single guide RNA plasmid for Cas9 targeting glpR in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards glpR in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. LENTICRISPR-TPI1_sgRNA1

    Plasmid

    #201626

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    TPI1 (TPI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. LENTICRISPR-TPI1_sgRNA2

    Plasmid

    #201627

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    TPI1 (TPI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. psgRNA-ccdB

    Plasmid

    #99915

    Purpose

    Basic cloning vector for the expression of imaging-based optimization sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p426_Cas9_gRNA-ARS106a

    Plasmid

    #87382

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. gRNA-CXCR4(90)

    Plasmid

    #98962

    Purpose

    Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Encodes a gRNA for CRISPR-mediated targeting of C…

    Promoter

    U6

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOsU3-esgRNA

    Plasmid

    #115629

    Purpose

    expression esgRNA in rice protoplasts

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    OsU3p-esgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197966

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCVp and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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