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Showing: 421 - 440 of 66158 results

  1. pME-Cas9-T2A-GFP

    Plasmid
    #63155
    Purpose
    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.
    Depositor
    Leonard Zon
    Publication
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Insert
    Cas9-T2A-GFP
    Use
    CRISPR; Tol2 middle entry vector for gateway
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    63155-plasmid-map-sequence-id-220487

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  3. pcDNA-dCas9

    Plasmid
    #47106
    Purpose
    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Flag and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive)
    Promoter
    CMV
    Available since
    Nov. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47106-plasmid-map-sequence-id-433987

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  10. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

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  11. pCEP4-M2L

    Plasmid
    #20926
    Purpose
    used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28
    Depositor
    James Thomson
    Publication
    Yu et al Science. 2009 Mar 26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    MYC (MYC Human)
    LIN28 (LIN28A Human)
    Use
    Episomal/ebna
    Promoter
    CMV and IRES
    Available since
    May 20, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20926-plasmid-map-sequence-id-9762

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  14. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

    Plasmid
    #83842
    Purpose
    Fluorescent probe for S/G2 transition and G2/M transition, one of two plasmids for the FUCCI4 system
    Depositor
    Michael Lin
    Publication
    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Insert
    mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83842-plasmid-map-sequence-id-179970

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  19. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid
    #124428
    Purpose
    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells
    Depositor
    Xiaokun Shu
    Publication
    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlipGFP(Casp3 cleavage seq) T2A mCherry
    Expression
    Mammalian
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124428-plasmid-map-sequence-id-322471

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Showing: 421 - 440 of 66158 results