We narrowed to 22,324 results for: Sis
-
Plasmid#154179Purposeexpresses spider monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro
Plasmid#192681PurposeLentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcriptionDepositorInsertMouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIR-D10 mutUTR
Plasmid#19116DepositorInsertHOXD10 mut 3' UTR (HOXD10 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAc-ÂHis-ÂzfTpo
Plasmid#64309PurposeExpress soluble His-tagged zebrafish thrombopoietin in insect cellsDepositorInsertThrombopoietin (thpo Zebrafish)
UseTags(His)6ExpressionInsectMutationThrombopoietin starts at AA37 (AA1-36 encode the …PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSuper mouse DGCR8-3
Plasmid#14774DepositorInsertDGCR8 shRNA (Dgcr8 Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPglu-600uDelta11 Aphrodite
Plasmid#13569DepositorInsertFLIPglu F16A Delta11 Aphrodite
UseTagsAphrodite and ECFPExpressionBacterialMutationFluorophore-derived linker shortened. F16A mutati…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0108
Plasmid#177041PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoterDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0817
Plasmid#177039PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoterDepositorInserttruncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R/F327A
Plasmid#104547PurposeExpresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/F327A
UseTagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
UKNP3.2.1
Plasmid#98222PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK1-2/3
Plasmid#91420PurposeProtein expression and purification of human SH3 domain construct NCK1-2/3DepositorInsertNCK1-2/3 (NCK1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3A-1/1
Plasmid#91478PurposeProtein expression and purification of human SH3 domain construct UBASH3A-1/1DepositorInsertUBASH3A-1/1 (UBASH3A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKF-D2irTNP2AG-T2APuroR-5kwh
Plasmid#128255PurposeMammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.DepositorInserthTetR::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNa22_opt.IRES.eGFP
Plasmid#22491DepositorInsertCodon optimised Calcineurin A mutant 22 (PPP3CA Human)
UseRetroviralTagsExpressionMutationV314R; Y341FPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
UKNP6.1.2
Plasmid#98388PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
UseTags3nmt1-mMaple3ExpressionBacterial and YeastMutationPromoter3nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ERBB4 (R544W)
Plasmid#29531DepositorInsertERBB4 (ERBB4 Human)
UseTagsExpressionMammalianMutationchanged arginine 544 to tryptophanPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.dTomato.miR-ctrl
Plasmid#169303PurposeConstitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent proteinDepositorInsertmiR-ctrl
UseLentiviralTagsdTomatoExpressionMutationPromoterSFFVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBGC155-MXMT
Plasmid#79818PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGC155/GFP (155–239)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
UKNP2.2.1
Plasmid#98209PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR01-FnCpf1
Plasmid#176529PurposeNuclease Cpf1 adapted for Aspergillus speciesDepositorInsertFnCpf1
UseCRISPR; Fungal expressionTagsExpressionMutationPromoterTEF1Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
2Bc-T MBP_BoMoC(ed)_I770A_6xH
Plasmid#185711PurposeRecombinant B. mori truncated R2 RT expressionDepositorInsertB. mori truncated R2 RT
UseTags6xHis and MBPExpressionBacterialMutationChanged I770 to APromoterT7Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B
Plasmid#120888PurposeExpresses Ptch1-B for function assaysDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS585P-myc
Plasmid#107498PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS585PPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-mWasabi-G4
Plasmid#164168PurposeExpresses mWasabi under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminalDepositorInsertmWasabi
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
miR-144 Trap
Plasmid#60934Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-144 Trap
UseLentiviralTagsGFPExpressionMutationPromoterbidirectional phosphoglycerate kinase promoter (b…Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXIP TGBRI K to R
Plasmid#19150DepositorInsertTransforming growth factor, beta receptor I (TGFBR2 Human)
UseRetroviralTagsHAExpressionMammalianMutationK420R mutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCarCBA
Plasmid#117754PurposeCodon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backboneDepositorInsertCodon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB427
Plasmid#74821PurposeGateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB426
Plasmid#74820PurposeGateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588D
Plasmid#48194PurposeExpresses L588D mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_L588A
Plasmid#48193PurposeExpresses L588A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-ATXN1_R665A
Plasmid#48195PurposeExpresses R665A mutated Ataxin1DepositorInsertAtaxin1 (ATXN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e-dimer
Plasmid#138501PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-eGFP
Plasmid#126720Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
UseTags6xHis,MycExpressionYeastMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M2-TLR2-V660N
Plasmid#12292DepositorInsertTLR2 (Tlr2 Mouse)
UseTagsFLAG (FLAG-M2)ExpressionMammalianMutationM82I, H202Y and V660N; signal peptide (aa1-23) re…PromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJMP1344
Plasmid#119273Purposeknockdown folA in E. aerogenesDepositorInsertsgRNA folA (E. aerogenes)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1274
Plasmid#119266PurposeB. subtilis deltaTn7 site and flanking sequence cloned into pACYCDepositorInsertB. subtilis deltaTn7
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1273
Plasmid#119265PurposeE. coli attTn7 site with B. subtilis flanking sequence cloned into pACYCDepositorInsertE. coli attTn7
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1263
Plasmid#119264PurposeB. subtilis attTn7 site and flanking sequence cloned into pACYCDepositorInsertB. subtilis attTn7
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1106
Plasmid#119249Purposeconstruction intermediateDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2d10-364delta263-295-TEV-STREP
Plasmid#171830PurposeMammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutation10-364delta263-295PromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
enAsABE8e-dimer
Plasmid#138507PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-dAsCas12a (En)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5B3-T
Plasmid#92021PurposeExpresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lysA with N-terminal TEV protease cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP2.5.1
Plasmid#98213PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTn
Plasmid#154369PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Neuroserpin
Plasmid#58259PurposeRetroviral vector for expressing Neuroserpin in mammalian cellsDepositorInsertNeuroserpin (SERPINI1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pmyo-3::tag:SL1(G20A)
Plasmid#81114PurposeExpression of exogenous SL1(G20A) in C. elegans muscleDepositorInsertsls-1.2 (sls-1.2 Nematode)
UseTagsillumina-adapterExpressionWormMutationG20APromoterpromoter of myo-3Available sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only