We narrowed to 10,115 results for: HA
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Plasmid#213516PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cellsDepositorInsertKU70-eGFP-SUNΔN
UseTagsExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA375
Plasmid#216054PurposeFragmid fragment: (C' terminus) HIV nuclear export sequence; HA tagDepositorHas ServiceCloning Grade DNAInsertHIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2-GFP (A1134-1135)
Plasmid#204203PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3 and eGFPExpressionMammalianMutationGly to Ala 1134, 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (STOP)
Plasmid#204204PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationNonsense mutations after Gly1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-2 (A1134-1135)
Plasmid#204201PurposeMammalian expression of SREBP-2DepositorInsertSREBP-2 (SREBF2 Human)
UseTagsT7x3ExpressionMammalianMutationGly to Ala 1134 to 1135PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAL4-Creb5 (1- 128)
Plasmid#195031PurposeGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)DepositorInsertGAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R(T308A)
Plasmid#208092PurposeMammalian expression of the human LTB4 receptor LTB4R with the T308A mutation with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R(T308A) (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationT308APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R(S310A)
Plasmid#208093PurposeMammalian expression of the human LTB4 receptor LTB4R with the S310A mutation with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R(S310A) (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationS310APromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_PGK_3xHA-LTB4R
Plasmid#208091PurposeMammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.DepositorInsert3xHA-LTB4R (LTB4R Human)
UseLentiviralTags3xHAExpressionMammalianMutationnonePromoterPGKAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 KATNAL2 residues 1-98
Plasmid#180619PurposeBacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.DepositorInsertKATNAL2 (KATNAL2 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 1-98PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 RPS6KC1 MIT, residues 235-316
Plasmid#180622PurposeBacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.DepositorInsertRPS6KC1 (RPS6KC1 Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 235-316PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LYK5_TM-3xHA
Plasmid#202191PurposeExpress citrus 'Tango' mandarin LYK5 gene under the UBQ10 promoterDepositorInsertLYSM-CONTAINING RECEPTOR-LIKE KINASE 5
UseTagsHAExpressionPlantMutationPromoterAtUBQ10Available sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 PSK (745-1235)
Plasmid#197863PurposeExpression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)DepositorInsertPSK1 (745-1235) (TAOK2 Human)
UseTagsMycExpressionMammalianMutationThe N terminus of PSK1/TAOK2 has been deletedPromoterSV40Available sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v2
Plasmid#178536PurposeSubcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backboneDepositorInsertC7orf26v2 (INTS15 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-2xFKBP (no insert) (pBS1139)
Plasmid#185322PurposeThe empty vector used for the FKBP experiments in our paperDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v1
Plasmid#178535PurposeSubcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backboneDepositorInsertC7orf26v1 (INTS15 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
UseTagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTAMAHISTEV_PfeAR480A
Plasmid#128944Purposeexpresses R480A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAG324V
Plasmid#128947Purposeexpresses G324V mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeEH258A
Plasmid#129122Purposeexpresses H258A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_Ab-PirA
Plasmid#128959Purposeexpresses Acinetobacter baumannii PirA in E. coliDepositorInsertferric enterobactin receptor PirA
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeES157A
Plasmid#129121Purposeexpresses S157A mutant of PfeE in E.coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removed, mutat…PromoterPBADAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson
Plasmid#117663PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT2)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_VPEVS
Plasmid#105863PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG3 with five mutat…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_ILGGP
Plasmid#105855PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG1 with five mutat…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgFFL_7x_Bulge
Plasmid#59894Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with seven bulge target sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has seven bulge targets (fully comple…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-R756S
Plasmid#60730PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756SDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSara-R756S
Plasmid#60725PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756S. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-Q758C
Plasmid#60724PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation Q758C. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
p6620 MSCV-IP N-HAonly AMPH
Plasmid#35016DepositorInsertAMPH (AMPH Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
35S:5mARF17 pLBR19
Plasmid#12073DepositorInsert5mARF17 (ARF17 Mustard Weed)
UseTagsExpressionPlantMutationmiR160-resistant version of ARF17 has five silent…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiVIPe4_Gq_dTomato
Plasmid#213858PurposeAAV construct expressing HA-hM3Dq-dTomato driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_Gq_dTomato
Plasmid#213832PurposeAAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe3_Gq_dTomato
Plasmid#213942PurposeAAV construct expressing HA-hM3Dq-dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WDP0003_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiSSTe4_Gq_dTomato
Plasmid#213946PurposeAAV construct expressing HA-hM3Dq-dTomato driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_Gq_dTomato
Plasmid#213938PurposeAAV construct expressing HA-hM3Dq-dTomato driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_Gq_dTomatoDepositorInsertHA-GqDREADD-dTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only