164,127 results

1. spNW30

   Plasmid

   #173485

   Purpose

   Expresses spNW30 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW30
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Caspase-3 (C163A)-pcw107-V5

   Plasmid

   #64633

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   Kris Wood, David Sabatini

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   CASP3 (transcript variant beta) (CASP3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   C163A

   Promoter

   PGK

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

3. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-vHras-zeo

   Plasmid

   #17756

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   vHras (genomic0 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V12D

   Promoter

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)-AKAP95-FRB

   Plasmid

   #138254

   Purpose

   Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   AKAP95-FRB (AKAP8 Human)
   

   Use

   Tags

   FRB rapamycin binding domain and HA epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTag-BFP-C-h-Rab7L1-c-Myc

   Plasmid

   #79803

   Purpose

   Express TagBFP labeled human Rab7L1.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab7L1 (RAB29 Human)
   

   Use

   Tags

   TagBFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TSHR-Tango

   Plasmid

   #66518

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   TSHR (TSHR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCVII-AM

   Plasmid

   #162751

   Purpose

   Retroviral (MSCV) expression of ametrine fluorescent protein

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   ametrine
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF0915

    Plasmid

    #143451

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA2 (NCOA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-mTH-GFP

    Plasmid

    #99128

    Purpose

    An AAV genome that expresses GFP from the mouse TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mTH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJM483

    Plasmid

    #52987

    Purpose

    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

    Depositor

    Ding Xue

    Article

    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

    Insert

    Lactaherin (Mfge8 Mouse)
    

    Use

    Tags

    GFP (w/ introns) and signal sequence

    Expression

    Worm

    Mutation

    deleted native signal sequence, deleted C-termina…

    Promoter

    hsp-16.41

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-UbcP-Myc-YWAA-pGK-HYG

    Plasmid

    #107377

    Purpose

    Lentiviral expression of myc-YWAA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    CRBN-Y383A/W385A

    Promoter

    UbcP

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TPC2-D276K-G-GECO1.2

    Plasmid

    #207142

    Purpose

    Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Changed Aspartate 276 to Lysine

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-CDT2

    Plasmid

    #215091

    Purpose

    Expresses FLAG-CDT2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDT2 (DTL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Flag-mRunx1c

    Plasmid

    #64894

    Purpose

    Lentiviral expression of Flag-tagged murine transcription factor Runx1c

    Depositor

    Georges Lacaud

    Article

    Batta et al Cell Rep. 2014 Dec 11;9(5):1871-84. doi:

    Insert

    Runx1c (Runx1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AbVec2.0-IGHG2

    Plasmid

    #99576

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-ccdB-V5

    Plasmid

    #214985

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ATGL*-V5-APEX2

    Plasmid

    #170574

    Purpose

    A C-terminal APEX2 fusion to the human ATGL S47A protein

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    ATGL* (PNPLA2 Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    changed serine 47 to alanine

    Promoter

    CMV

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PresentER-SIINFEKL (mCherry)

    Plasmid

    #102945

    Purpose

    PresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    SIINFEKL (OVAL Synthetic, Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    OVA257-264

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

26. pAAV INS2x mRFP-3MREs (AAV-beta)

    Plasmid

    #206928

    Purpose

    Beta cell-specific gene expression from AAV in human pancreatic islets

    Depositor

    Markus Grompe

    Article

    Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INSx2

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pD21-AI-hLDLR

    Plasmid

    #124964

    Purpose

    Express human LDLR under human APOAI promoter

    Depositor

    Kazuhiro Oka

    Article

    Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:1

    Insert

    human LDLR (LDLR Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    human APOAI

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBactin-mNeonGreen-CI

    Plasmid

    #196850

    Purpose

    Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry

    Plasmid

    #85434

    Purpose

    Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLJC6-2XFLAG-TMEM192

    Plasmid

    #104435

    Purpose

    Expresses 2XFLAG-TMEM192 in mammalian cells

    Depositor

    David Sabatini

    Article

    Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

    Insert

    TMEM-192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-Neq2X7

    Plasmid

    #182366

    Purpose

    Expresses Neq2X7 DNA polymerase

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

    Insert

    Neq2X7 DNA polymerase
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMC3-yeGFP

    Plasmid

    #176181

    Purpose

    yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced GFP

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    yEGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDY1623 NOLC1 sgRNA 2

    Plasmid

    #234834

    Purpose

    sgRNA 2 for NOLC1 STITCHR insertion

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

    Plasmid

    #214885

    Purpose

    Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234452

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. eGFP-KIF1A

    Plasmid

    #172206

    Purpose

    Expresses KIF1A tagged with eGFP in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-C1-ER beta

    Plasmid

    #28237

    Depositor

    Michael Mancini

    Insert

    ER beta (ESR2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTA7001-DEST

    Plasmid

    #71745

    Purpose

    GATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducible

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GAL4

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Tau (P301L)-VC

    Plasmid

    #87633

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Mutation P301L

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLV-U6-UL29-egfp-U3-UL8

    Plasmid

    #166694

    Purpose

    To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (EGFP version).

    Depositor

    Yujia Cai

    Article

    Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

    Insert

    UL8 and UL29 gRNA, EGFP (UL8, UL29 HSV-1)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge