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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 40,667 results for: TRO

Showing: 11251 - 11300 of 40667 results

  11. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  12. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  14. Dox-SOScat

    Plasmid
    #207329
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    son of sevenless homolog 2 variant, catalytic domain
    Use
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 593 to 1078
    Promoter
    Tet Response Element
    Available since
    Nov. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    207329-plasmid-map-sequence-id-413071

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  25. pBMN BFP

    Plasmid
    #188644
    Purpose
    This is the Lazarou lab's standard retrovirus vector backbone for expression of BFP-tag fusions onto the N-terminus of cloned genes
    Depositor
    Michael Lazarou
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    BFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    188644-plasmid-map-sequence-id-370207

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  27. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  28. JAB3301

    Plasmid
    #190405
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO(internal)::GFPint-ADHter)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO(internal)::GFPint-ADHter
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    190405-plasmid-map-sequence-id-391300

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  31. JAB2515

    Plasmid
    #190383
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-TetRO::GFPint-NLS-ADHt)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-TetRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    190383-plasmid-map-sequence-id-389486

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  32. JAB2275

    Plasmid
    #190361
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    190361-plasmid-map-sequence-id-389441

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  34. pQE80l BCB

    Plasmid
    #196472
    Purpose
    Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher
    Depositor
    Fei Sun
    Article
    Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114
    Insert
    BCB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196472-plasmid-map-sequence-id-387266

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Showing: 11251 - 11300 of 40667 results