We narrowed to 12,334 results for: pcdna

Showing: 6781 - 6810 of 12334 results

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127315

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187089

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter hsa-mir-122

Plasmid

#46673

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir-122 (MIR122 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-GSG-SpyTag003

Plasmid

#216292

Purpose

Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-GSG-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

Plasmid

#194385

Purpose

Gateway Destination vector for split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

attR1-ORF-attR2-NTEV-TCS-GV-2xHA

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-Ccmut (N202P-V361P)

Plasmid

#225457

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (N202P V361P)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N202P V361P

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

Plasmid

#200980

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4107-4303)-GST

Plasmid

#83456

Purpose

Human PC1-CTp30 expression cassette with a GST-tag (678)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR

Plasmid

#187091

Purpose

GABA sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-iGABASnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR

Plasmid

#187092

Purpose

GABA sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-iGABASnFR

Tags

iRFP670-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

Plasmid

#175421

Purpose

Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR

Plasmid

#187090

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Tags

iRFP670-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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1129 pcDNA3 GFP FKHR H215R AAA

Plasmid

#9034

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR H215R AAA (FOXO1 Human)

Tags

GFP

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79619

Purpose

Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

3xFlag-PmCDA1

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-544 delta166-172

Plasmid

#53551

Purpose

mammalian expression of TPP1 full length with 166-172 replaced with "GSSG"

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

contains aa 88-544, with aa 166-172 replaced wit…

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79621

Purpose

Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Mammalian

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pCMV

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

Plasmid

#115843

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx-Q76A

Tags

3x-FLAG

Expression

Mammalian

Mutation

Q76A

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Plasmid

#160742

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop-exon minimal CMV pCDNA5

Plasmid

#212080

Purpose

LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase Non-Cleavable

Plasmid

#59826

Purpose

Allows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Non-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

Plasmid

#160741

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4,K852A)

Tags

HA-Tag

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-ΔD-box-CycA-GFP (525)

Plasmid

#39856

Depositor

Jonathon Pines

Article

den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Insert

Cyclin A2 (CCNA2 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

L2V; Δ destruction box = deletion of aa47-55

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

Plasmid

#123338

Purpose

Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

DAKAP-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal 30 amino acids from DAKAP1, Venus, and…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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9E10.mCHOP(S78&81A).pCDNA1/Amp

Plasmid

#21902

Depositor

David Ron

Insert

CHOP (Ddit3 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

S78A S81A

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

Plasmid

#62977

Purpose

Expresses myc-tagged mouse βTrCP residues 241-569

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

BTRC (Btrc Mouse)

Tags

myc

Expression

Mammalian

Mutation

codes for residues 241-569

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only