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Showing: 661 - 690 of 159111 results

  1. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

    Plasmid
    #199776
    Purpose
    enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    R297T for reduced recombinase efficiency.
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    199776-plasmid-map-sequence-id-391713

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  4. rtTA-N144

    Plasmid
    #66810
    Purpose
    2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance
    Depositor
    Andrew Yoo
    Article
    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi
    Insert
    Reverse tetracycline-controlled transactivator
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1a
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    66810-plasmid-map-sequence-id-418923

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  6. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  10. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  16. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  17. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  22. pSL1-GFP

    Plasmid
    #180420
    Purpose
    Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a GFP promoter.
    Depositor
    Nancy Moran
    Article
    Li et al Proc Natl Acad Sci U S A. 2022 May 3;119
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    180420-plasmid-map-sequence-id-357217

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  28. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid
    #29655
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, GST, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    29655-plasmid-map-sequence-id-196920

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  29. pSIJ8

    Plasmid
    #68122
    Purpose
    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression
    Depositor
    Alex Nielsen
    Article
    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103
    Inserts
    flp recombinase
    rhaS
    rhaR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rhamnose
    Available since
    Dec. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68122-plasmid-map-sequence-id-243302

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Showing: 661 - 690 of 159111 results