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Plasmid#202608PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P-Kv
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-VP64-dCas9-VP64_Blast
Plasmid#192650Purpose3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance markerDepositorInsertVP64-dCas9-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-donor_opt(R2Tg)-GFP(intron)
Plasmid#223252PurposeMammalian expression of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOTTC374 - pAAV EF1a DIO iRFP
Plasmid#47626PurposeAn AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).DepositorInsertInfrared fluorescent protein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Flex-Axon-GCaMP7b
Plasmid#135419PurposeCan be used to drive axon-enriched expression of GCaMP7b in the presence of Cre recombinase.DepositorInsertaxon-jGCaMP7b
UseAAVTags6xHis and GAP43 palmitoylation domainExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-EF1a ANXA11-mEmerald D40G
Plasmid#164211PurposepLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoterDepositorInsertANXA11 (ANXA11 Human)
UseLentiviralTagsmEmeraldExpressionMutationD40GPromoterEF1aAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-CAG-hMGT-Ef1a-puro
Plasmid#172395PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-HA-hTet1CD-WPRE-PolyA
Plasmid#39454DepositorInsertHA-hTet1CD (TET1 Human)
UseAAVTagsHAExpressionMammalianMutationaa1418–2136PromoterEF1aAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-ASAP3-WPRE
Plasmid#132318Purposeexpresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vectorDepositorInsertASAP3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-UBCp-hMGT-Ef1a-puro
Plasmid#172396PurposeLentiviral expression vector for human direct cardiac reprogrammingDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-EGFP-WPRE-pA
Plasmid#37091PurposeExpresses EGFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertd1eGFP
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
P-Ef1a-EGFP-T2A-RPL22-1xFlag
Plasmid#170323PurposeExpresses FLAG and EGFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO ChETA-EYFP
Plasmid#26968PurposeAAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activationDepositorHas ServiceAAV1, AAV5, and AAV9InserthChR2(E123T/H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationE123T, H134RPromoterEF1alphaAvailable sinceJan. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1alpha-IL3-218-PDGFR
Plasmid#139055PurposeA lentivirus vector to integrate a surface membrane anchored human IL3 geneDepositorInsertinterleukin 3(IL3) (IL3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Neo-EF1A>Tet3G
Plasmid#184379PurposeThe Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)DepositorInsertTet3G
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-TAL1-IRES-Puro
Plasmid#61065Purposelentiviral vector for TAL1DepositorInsertTAL1 (TAL1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterEF1alphaAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-HygroR-T2A-TetOn3G
Plasmid#179887PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsHygromycin resistanceExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP453 pEf1a-NLS-aGCN4-mCherry
Plasmid#100945PurposemCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tagDepositorInsertscFvGCN4, sfGFP, GB1, mCherry
UseTags3xTy1ExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-PPO-Venus
Plasmid#139505PurposeAAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.DepositorHas ServiceAAV8 and AAV9Insertparapinopsin
UseAAVTagsVenus tag following 3x Ala linkerExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Puro
Plasmid#135683PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9.EF1a-BFP.sgLMNA
Plasmid#98971PurposeCas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.DepositorInsertLMNA sgRNA and spCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-loxP-GRAB_DA2m-loxP
Plasmid#189751PurposeExcludes sensor expression in Cre-positive neuronsDepositorInsertDopamine sensor GRAB_DA2m
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-FLEx-ASAP4b-Kv-WPRE
Plasmid#201032PurposeExpresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent mannerDepositorInsertASAP4e-Kv
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE
Plasmid#179459PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorHas ServiceAAV1InsertJEDI-2P-Kv
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.28.3z
Plasmid#200674PurposeModular CD8hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-mKate-NGFR
Plasmid#104831PurposeextGluc luciferase for invivo Imaging, mKate and NGFR for flourescent detectionDepositorInsertsmKate
NGFR
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#202610PurposeDouble floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro PFKM
Plasmid#201638PurposeOver-expression of untagged human PFKMDepositorInsertPFKM (PFKM Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-TVA-mCherry
Plasmid#38044DepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
UseAAV and Cre/LoxTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GalT-IRES-Puromycin
Plasmid#134862PurposeExpresses EGFP-tagged Golgi markerDepositorInsertbeta 1,4-galactosyltransferase (B4GALT1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 83-398PromoterEF1aAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.16_ M4_Ef1a-gateway-V5-SV40-Zeo
Plasmid#128452Purposelentiviral destination vector with Ef1a promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI
Plasmid#159756PurposePiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backboneDepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1alpha-EPO-218-PDGFR
Plasmid#139057PurposeA lentivirus vector to integrate a surface membrane anchored human EPO geneDepositorInserterythropoietin(EPO) (EPO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CVS-G-WPRE-pGHpA
Plasmid#67528Purpose"Expresses the rabies glycoprotein"DepositorInsertRabies virus strain CVS-11 glycoprotein (G)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FRT-EF1a-TVA-2A-N2cG
Plasmid#172361PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTDSS1-IRES-Puromycin
Plasmid#134667PurposeExpresses EGFP-tagged Mitochondria-associated ER membrane markerDepositorInsertPTDSS1 (PTDSS1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO SwiChRca-TS-EYFP
Plasmid#55631PurposeCre-Dependent Chloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationC128APromoterEf1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-LMO2-IRES-Puro
Plasmid#61064Purposelentiviral vector for LMO2DepositorInsertLMO2 (LMO2 Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1alphaAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only