159,258 results
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Plasmid#91918PurposeExpresses LbfCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLbfCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNJB91
Plasmid#51519PurposeGateway entry vector with attL1 and attR5 sites. Between the att sites are two FokI coding sequences transcriptionally linked by a T2A sequence. There is a nos-T after the last FokI.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
UseTagsHistidineExpressionBacterial, Insect, and Mamm…MutationPromoterCMV ie enhancer/promoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTG06-Amp-FRT
Plasmid#128275PurposeDeg-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG-P2A-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_a_tubulin_IRES_puro2
Plasmid#21043DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
p15a-AsCas12f-blaZ
Plasmid#171609PurposeAsCas12f1-based E. coli genome editingDepositorInsertAsCas12f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Integrin-Alpha2-N-18
Plasmid#55063DepositorInsertIntegrin-Alpha2 (ITGA2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRN114
Plasmid#127221PurposeBeYDV replicon with constitutive Luc expression, WUS2 and AtSTMDepositorInsertLuc, Wus, AtSTM
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-(-2)AP-Ub
Plasmid#232582PurposeExpress Ubiquitin tagged with a modified Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAPExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirAonly
Plasmid#232584PurposeExpress HA-BirADepositorInsertBirA
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP-Ub-dGG
Plasmid#232581PurposeExpress Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycinesDepositorInsertUbiquitin
UseTagsAviTagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP(-3)-Ub
Plasmid#232583PurposeExpress Ubiquitin tagged with a modified Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP-Ub
Plasmid#232577PurposeExpress Ubiquitin tagged with an Acceptor Peptide (AP)DepositorInsertUbiquitin
UseTagsAviTagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-CHIP-GSGS-BirA-HA
Plasmid#232588PurposeExpress BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.DepositorInsertCHIP - BirA (CHIP Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_212
Plasmid#91111PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP Sec22b
Plasmid#101918PurposeExpresses Sec22b in mammalian cellsDepositorInsertSec22b (Sec22b Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom70 (1-70)-GFP
Plasmid#127633PurposeMitochondrial targeting sequence of Tom70 fused to GFPDepositorInsertTom70 (TOMM70 Human)
UseTagseGFPExpressionMammalianMutationOnly first 70 amino acidsPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA2
Plasmid#85710PurposeThis plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomesDepositorTypeEmpty backboneUseCre/Lox; Herpesvirus cloningTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNestin-EGFP
Plasmid#38777DepositorInsertnestin 2nd intron enhancer with TK mini promoter (NES Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNJB80
Plasmid#51518PurposeGateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZyxin-mMaple3
Plasmid#101151PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorInserthuman codon optimized mMaple3 fused to zyxin
UseTagsExpressionMammalianMutationPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RLuc8
Plasmid#87121PurposeMammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.DepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationA55T, C124A, S130A, K136R, A143M, M185V, M253L, a…PromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X1
Plasmid#59200PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt1-ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)
Plasmid#175574PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG1.1
Plasmid#173685PurposeThe expression vector for genes of interest under CAG promoter.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_WT
Plasmid#83482PurposeBacterial expression of WT L. buccalis C2c2 CRISPR effector.DepositorInsertLbu_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN_HA-TBK1 (kinase dead - D135N)
Plasmid#199206PurposeUsed for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationD135NPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-wt
Plasmid#12975DepositorInsertCdc42 (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSM-mStayGold
Plasmid#234317Purposecodon-optimized mStayGold with synthetic introns for the expression in C. elegansDepositorInsertC. elegans codon optimized mStayGOld
UseTagsmStayGoldExpressionWormMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-SnxA
Plasmid#232460PurposeLentiviral EGFP lipid sensor for PI(3,5)P2DepositorInsertEGFP-SnxA
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12799 - pAAV-AiE0170h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2799)
Plasmid#230132PurposeAiE0170h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP12839 - pAAV-AiE0589m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2839)
Plasmid#230136PurposeAiE0589m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-EcTrp-TGA
Plasmid#228780PurposeaaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codonDepositorInsertsEcTrpRS
EcWtR
UseBaculoviralTagsExpressionMammalianMutationTGA suppressorPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mGFP in pRS315
Plasmid#221158PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmGFPExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI in pRS315
Plasmid#221159PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-NG-CBE
Plasmid#227420PurposeC-to-T base editing in mammalian cellsDepositorInsertTadA-nSpCas9-NG-2xUGI (AICDA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only