We narrowed to 16,985 results for: She

1. EcNGsC-F307A-pKK223

   Plasmid

   #131382

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera F307A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are from…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EcNGsC-S308V-pKK223

   Plasmid

   #131393

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308V
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EcNGsC-S308A-pKK223

   Plasmid

   #131394

   Purpose

   Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

   Depositor

   Martin Horvath

   Article

   Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

   Insert

   EcNGsC MutY chimera S308A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fusion of two MutY genes. Residues 1-225 are fro…

   Promoter

   tac

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBacMam2-DiEx-LIC-N entry vector

   Plasmid

   #111751

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

   Plasmid

   #112986

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331D

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

   Plasmid

   #112988

   Purpose

   P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

   Insert

   mei-S332 (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Expression

   Mutation

   T331A

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMAL c2x Tral N

   Plasmid

   #113009

   Purpose

   For bacterial expression of MBP fusion of N terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   N terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 1-355

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAL c2x Tral FL-15A

   Plasmid

   #113011

   Purpose

   For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   full length tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   15 PNG phosphorylation sites mutated to A

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMAL c2x Tral C

   Plasmid

   #113008

   Purpose

   For bacterial expression of MBP fusion of C terminal region of Drosophila Tral

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

   Insert

   C terminus of tral (tral Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   amino acids 356-652

   Promoter

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMAL c2x Tral C 15A

    Plasmid

    #113007

    Purpose

    For bacterial expression of MBP fusion of C terminal region of Drosophila Tral phosphomutant (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    C terminus of tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid

    #80638

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 217 to Alanine

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid

    #80637

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Trp 220 to Ala

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid

    #80639

    Purpose

    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4A (CHMP4A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 205-222; Changed Leucine 214 to Alanine

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. csd6_17-330/pET28b(+) N-tag

    Plasmid

    #71353

    Purpose

    csd6, construct:17-330, pET28b(+) N-tag (Please note that this plasmid has a C-terminal His tag)

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 )
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

15. csd6_13-330_E110A/pET28b(+) N-tag

    Plasmid

    #71354

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:E110A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Glutamic acid 110 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

16. csd6_13-330_Y133A/pET28b(+) N-tag

    Plasmid

    #71356

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y133A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 133 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

17. csd6_13-330_C176A/pET28b(+) N-tag

    Plasmid

    #71360

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:C176A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Cysteine 176 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

18. csd6_13-330_H160A/pET28b(+) N-tag

    Plasmid

    #71359

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 160 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. csd6_13-330_W158A/pET28b(+) N-tag

    Plasmid

    #71358

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tryptophan 158 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. csd6_13-330_H155A/pET28b(+) N-tag

    Plasmid

    #71357

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 155 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

21. csd6_13-330_Y132A/pET28b(+) N-tag

    Plasmid

    #71355

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:Y132A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Tyrosine 132 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pLJM60-FLAG-SLC38A9.1 Y71A

    Plasmid

    #71865

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, Tyrosine 71 mutated to Alanine

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pCDF1-ADAMTS18 (Q1002X)

    Plasmid

    #29557

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 1002 to stop codon

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pCDF1-ADAMTS18 (Q904X)

    Plasmid

    #29556

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glutamine 904 to stop codon

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pCDF1-ADAMTS18 (G312E)

    Plasmid

    #29553

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 312 to glutamic acid

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pCMV5b HA Irx5 deltaHD

    Plasmid

    #24997

    Depositor

    Benoit Bruneau

    Article

    Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

    Insert

    Irx5 (Irx5 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletes entire HD (aa 112-177)

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LP110a

    Plasmid

    #90199

    Purpose

    pEGFP-N1 with cDNA encoding full length HsKIF13A

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    KIF13A (KIF13A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

    Plasmid

    #229845

    Purpose

    A CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Lamin nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGP-AAV-syn-jGCaMP8m-WPRE

    Plasmid

    #162375

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYLCRISPR/Cas9Pubi-B

    Plasmid

    #66188

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. hfCas12Max

    Plasmid

    #195336

    Purpose

    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized hfCas12Max
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    N243R, E336R, D892R

    Promoter

    CBh, CMV, hU6

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pC-G418-YR

    Plasmid

    #61767

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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42. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV_GFAP_HyPer7_mito

    Plasmid

    #238966

    Purpose

    Mammalian astrocytes HyPer7 expression targeted to the mitochondrial matrix

    Depositor

    Dmitry Bilan

    Article

    Kotova et al Free Radic Biol Med. 2023 Nov 1;208:153-

    Insert

    HyPer7
    

    Use

    AAV

    Tags

    3x Cytochrome C Oxidase Subunit VIII presequense

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. shYAP1 # 2

    Plasmid

    #42541

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. dCas12Max-ABE

    Plasmid

    #195335

    Purpose

    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-ABE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti6.2_3xFLAG_V5_ mmDdx58_V5

    Plasmid

    #160108

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLV-mEGFP-hCTNNA1_Wild-type

    Plasmid

    #234551

    Purpose

    Human a-Ecat sequence/CTNNA1

    Depositor

    Cara Gottardi

    Article

    Quinn et al Mol Biol Cell. 2024 May 1;35(5):ar65. do

    Insert

    mEGFP:hCTNNA1_Wild-type (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    None

    Promoter

    RSV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRK5-LAMP1-FLAG

    Plasmid

    #71868

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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