Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

169,724 results

Showing: 6931 - 6960 of 169724 results

  1. pD-MSP1D1

    Plasmid
    #161607
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    D-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans were replaced b…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161607-plasmid-map-sequence-id-317653

    Enlarge

  6. mTurquoise2-pBAD

    Plasmid
    #54844
    Purpose
    Localization: Protein Expression Vector , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6x His, T7 tag, and TEV site
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54844-plasmid-map-sequence-id-139002

    Enlarge

  7. p406MET3

    Plasmid
    #17436
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    MET3 promoter is between SacI and XbaI restrictio…
    Available Since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17436-plasmid-map-sequence-id-8610

    Enlarge

  8. pLenti.puro.MCV.ER.RAZ.2

    Plasmid
    #114382
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=428 aa.
    Depositor
    Reety Arora, Sudhir Krishna
    Article
    Arora et al Microb Pathog. 2019 Dec;137:103778. doi:
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    MCV NCCR (viral own promoter)
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114382-plasmid-map-sequence-id-221614

    Enlarge

  10. pcDNA3-CFP

    Plasmid
    #13030
    Purpose
    Mammalian expression vector for cloning your gene fused to CFP
    Depositor
    Doug Golenbock
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13030-plasmid-map-sequence-id-212096

    Enlarge

  11. pD649-HAsp-TEK-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156957
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    TEK (TEK Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156957-plasmid-map-sequence-id-382014

    Enlarge

  14. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

    Enlarge

  15. PEAR1-bio-His

    Plasmid
    #51860
    Purpose
    Expresses full-length Platelet endothelial aggregation receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PEAR1 (PEAR1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51860-plasmid-map-sequence-id-79749

    Enlarge

  16. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

    Enlarge

  20. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

    Enlarge

Showing: 6931 - 6960 of 169724 results