163,682 results

1. PPDK

   Plasmid

   #64037

   Purpose

   Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

   Depositor

   Vern Schramm

   Article

   Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

   Insert

   pyruvate phosphate dikinase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMSCVpuro-mTagBFP2

   Plasmid

   #96935

   Purpose

   Retroviral plasmid containing mTagBFP2 for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Monomeric Tag Blue Fluorescent Protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIM28 gRNA (BRDN0001162487)

   Plasmid

   #76139

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-cj-E sgRNA

   Plasmid

   #169915

   Purpose

   Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

   Plasmid

   #238240

   Purpose

   For integration of mCherry_P2AT2A_GFP-nb-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-KS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbA5a-sufABCDSE

   Plasmid

   #195056

   Purpose

   Expresses SUF operon from E. coli from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufABCDSE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNANOG-Luc

   Plasmid

   #25900

   Depositor

   Ren-he Xu

   Article

   Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

   Insert

   NANOG promoter (NANOG Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-RelA

   Plasmid

   #23255

   Depositor

   Warner Greene

   Article

   Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

   Insert

   p65 (RELA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. BRCA1 A10.2 gRNA

   Plasmid

   #90549

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA1 (Guide Designation A10.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.hSynap.ArcLightD.WPRE.SV40

    Plasmid

    #100037

    Purpose

    AAV expression of ArcLightD driven by hSynap promoter

    Depositor

    Vincent Pieribone

    Insert

    ArcLightD
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynap

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO1

    Plasmid

    #52258

    Purpose

    Bacterial expression of human 6His-tagged SUMO1, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-1 (SUMO1 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 )
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0098

    Plasmid

    #142460

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MSGN1 (MSGN1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK289 (mAID-mClover-NeoR)

    Plasmid

    #72827

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Neo
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

    Plasmid

    #72638

    Purpose

    Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain

    Depositor

    Joshua Bonkowsky

    Insert

    UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mVenus N1

    Plasmid

    #27793

    Depositor

    Steven Vogel

    Article

    Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

    Has Service

    Cloning Grade DNA

    Insert

    mVenus N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. nls-EGFP

    Plasmid

    #67652

    Purpose

    Mammalian expression vector driving nls-EGFP.

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    nls-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHSP02

    Plasmid

    #117259

    Purpose

    Genome editing for Lactobacillus plantarum WCSF1

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTK305

    Plasmid

    #66812

    Purpose

    IVT vector for Fluc mRNA

    Depositor

    Ron Weiss

    Article

    Andries et al J Control Release. 2015 Sep 2. pii: S016

    Insert

    Firefly luciferase
    

    Use

    Luciferase; Mrna ivt

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDAC439

    Plasmid

    #195237

    Purpose

    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR223-JAK3

    Plasmid

    #23944

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

    Plasmid

    #217713

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-743

    Promoter

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

    Plasmid

    #217712

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

    Plasmid

    #217711

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-6

    Promoter

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CAG-emiRFP670

    Plasmid

    #197200

    Purpose

    Expresses the protein of emiRFP670 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    emiRFP670
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. lentiGuide-Hygro-eGFP

    Plasmid

    #99375

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge