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167,150 results

Showing: 6931 - 6960 of 167150 results

  1. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available Since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pGEX6P1-DEST-His

    Plasmid
    #119763
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    10xHis and GST
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119763-plasmid-map-sequence-id-234026

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  17. pHM106Env

    Plasmid
    #139024
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein (env HIV-1)
    Expression
    Mammalian
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    139024-plasmid-map-sequence-id-288127

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  18. FokI-dCas9

    Plasmid
    #52970
    Purpose
    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells
    Depositor
    David Liu
    Article
    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103
    Insert
    Fok1 fused to dCas9
    Use
    CRISPR
    Tags
    Fok1 nuclease (homodimeric wildtype) and NLS
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9
    Promoter
    CMV
    Available Since
    May 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52970-plasmid-map-sequence-id-82408

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  27. pC1_oROS-HT

    Plasmid
    #216413
    Purpose
    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.
    Depositor
    Andre Berndt
    Article
    Lee et al bioRxiv 2024.02.06.579232
    Insert
    oROS-HT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216413-plasmid-map-sequence-id-435275

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  29. pUDP002

    Plasmid
    #103872
    Purpose
    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Inserts
    hph
    Spcas9 D147Y P411T
    Use
    CRISPR
    Tags
    NLS
    Expression
    Yeast
    Mutation
    D147Y P411T
    Promoter
    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    103872-plasmid-map-sequence-id-199260

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Showing: 6931 - 6960 of 167150 results