173,273 results
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Plasmid#46756PurposeLuciferase reporter gene for factors with GAL4 DNA binding domainsDepositorInsertLuciferase
UseLuciferasePromoterminimal TATAAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.Available SinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET15b_LbuCas13a-6xHIS
Plasmid#251213PurposeBacterial overexpression and recombinant protein purification of LbuCas13a.DepositorInsertLbuCas13a
TagsHISExpressionBacterialPromoterlacAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NGN2-EMX1 BSD-mApple
Plasmid#182311PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mAppleTagsT2A-mycNLS-mAppleExpressionMammalianPromoterEF1a and TRE3GAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLX-CHOP-dGFP
Plasmid#71299PurposeLentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoterDepositorInsertdGFP
UseLentiviralPromoterCHOP (ATF-responsive)Available SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIIS1011
Plasmid#238212PurposeExpresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.DepositorInsertParTasR
TagsHA-NLSExpressionMammalianAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonExpressionMammalianAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato
Plasmid#107738PurposeCan be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre, dTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhSynAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HDR-mEGFP-Actin
Plasmid#119870PurposeAAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta ActinDepositorInsertbeta Actin (Actb Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Gsk3b
Plasmid#123592DepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-GFP-IRF3
Plasmid#127663PurposeOver-express EGFP-IRF3DepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(F74G)-V5
Plasmid#158664PurposeTet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(F74G) AAVS1
UseCRISPRExpressionMammalianMutationF74GAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
Plasmid#32627DepositorInsertDonor + Sce BFP
UseLentiviralAvailable SinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
Tags10xHisExpressionBacterialAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD
Plasmid#171238PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_HyPer7_mito
Plasmid#238968PurposeMammalian neurons HyPer7 expression targeted to the mitochondrial matrixDepositorInsertHyPer7
UseAAVTags3x Cytochrome C Oxidase Subunit VIII presequenseExpressionMammalianPromoterhSyn1Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGS-21a his6-GST-WTPhi29DNAP
Plasmid#166863PurposeTo produce a dual affinity-tagged Phi29 DNA polymeraseDepositorInsertPhi29 Polymerase codon-optimized for overexpression in E. coli
TagsGST and his6ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BZLF1(Zta)
Plasmid#195787PurposeGateway Entry Clone of Epstein-Barr virus BZLF1DepositorInsertBZLF1 (BZLF1 Epstein-Barr virus)
UseGateway CloningAvailable SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1
Plasmid#233078PurposeExpression vector for sgRNA-S1b and eCas12f1DepositorInsertU6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP
UseCRISPRTags2xFLAGExpressionMammalianPromoterhU6, CBhAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cre-MS2_UbC-GFP (Cre_GFP)
Plasmid#219536Purposea. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotorDepositorAvailable SinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRX-IP-HaloTag7-LC3
Plasmid#184899PurposeStable expression of HaloTag7-LC3 in mammalian cells to measure autophagic fluxDepositorInsertLC3B
TagsHaloTag7ExpressionMammalianAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human Integrin beta 2
Plasmid#8640DepositorInsertIntegrin beta 2 (ITGB2 Human)
ExpressionMammalianAvailable SinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
TATA-GFP
Plasmid#110833Purposenegative controlDepositorInsertGFP
UseLentiviralExpressionMammalianAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Plac-dCas9 (Plac)
Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599AAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertVillin-mTQ2-P2A-FlpO-ERT2
TagsmTQ2, linked to FlpO through an P2A ribosomal ski…ExpressionMammalianPromoterVillinAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRExpressionMammalianAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only