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We narrowed to 1,198 results for: e.coli

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Showing: 1151 - 1198 of 1198 results

  3. pcDNA-CasE-p300-Flag

    Plasmid
    #106275
    Purpose
    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Insert
    EcoCasE-p300
    Use
    CRISPR
    Tags
    E tag, NLS and Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    106275-plasmid-map-sequence-id-247907

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  4. pRBC-PINBodyN-Tau(1-441)-404TAG

    Plasmid
    #253347
    Purpose
    Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 1-441, TAG codon at residue 404 for phosphoserine encoding
    Depositor
    Richard Cooley
    Article
    Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:
    Insert
    Tau 2N4R S404 TAG (MAPT Human)
    Tags
    PINBodyN (his6-tag included)
    Expression
    Bacterial
    Mutation
    Serine 404 to TAG codon
    Promoter
    T7
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253347-plasmid-map-sequence-id-508709

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  6. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid
    #222288
    Purpose
    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.
    Depositor
    Anne Straube
    Article
    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-
    Insert
    KIF1C (KIF1C Human)
    Tags
    6xHis, GFP, and GST
    Expression
    Bacterial
    Mutation
    stalk region only amino acids 642 to 922
    Promoter
    T7
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222288-plasmid-map-sequence-id-475373

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  26. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid
    #182671
    Purpose
    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.
    Depositor
    Bas van Steensel
    Article
    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182671-plasmid-map-sequence-id-450317

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  31. pRBC-PINBodyC-Tau(297-441)-404 TAG-his6

    Plasmid
    #253345
    Purpose
    Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441, TAG codon at residue 404 for phosphoserine and non-hydrolyzable analog of phosphoserine encoding
    Depositor
    Richard Cooley
    Article
    Vesely et al Protein Sci. 2026 Apr;35(4):e70520. doi:
    Insert
    Tau 2N4R S404TAG (MAPT Human)
    Tags
    His6 and PINBodyC
    Expression
    Bacterial
    Mutation
    Serine 404 to TAG codon, deleted amino acids 1-296
    Promoter
    T7
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253345-plasmid-map-sequence-id-508701

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  42. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid
    #230043
    Purpose
    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.
    Depositor
    Sumeet Pal Singh
    Article
    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:
    Insert
    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    fabp10a
    Available Since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    230043-plasmid-map-sequence-id-461059

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  43. pMG051 MBP-β-catenin-His, GST-CK1α

    Plasmid
    #154072
    Purpose
    Co-expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) and GST_CK1α in E.coli to produce primed MBP-β-catenin-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.
    Inserts
    CTNNB1 (CTNNB1 Human)
    CSNK1A1 (CSNK1A1 )
    Tags
    GST, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    154072-plasmid-map-sequence-id-308806

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  44. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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  45. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Article
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Available Since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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  46. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  48. pBAF

    Plasmid
    #104152
    Purpose
    Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.
    Depositor
    Robert Craigie
    Article
    Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95
    Insert
    Barrier-to-autointegration factor (BAF) (BANF1 Human)
    Tags
    N-terminal His tag with thrombin cleavage site
    Expression
    Bacterial
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    104152-plasmid-map-sequence-id-201395

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Showing: 1151 - 1198 of 1198 results