173,273 results

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pEGFP_TOMM20_sfCherry2(11)

Plasmid

#83033

Purpose

Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

TOMM20_sfCherry+11

Expression

Mammalian

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT3

Plasmid

#23895

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT3 (FLT3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGs-RFP

Plasmid

#127347

Purpose

Expresses RFP constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Insert

Red Fluorescent Protein

Expression

Mammalian

Promoter

CAGGS Promoter

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDY0663 ACTB atgRNA with v2 scaffold

Plasmid

#179108

Purpose

ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Green Lindoblum

Plasmid

#163113

Purpose

Expresses biosensor for lactate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Lindolum

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAM5409

Plasmid

#132662

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Expression

Bacterial

Promoter

PaadA and PconII

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-SsAPOBEC3B

Plasmid

#198536

Purpose

Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-2BD

Plasmid

#234428

Purpose

Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

Depositor

Min-Kyu Oh

Article

Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

Insert

Modified Cas9 with polyaspartate linking

Expression

Bacterial

Mutation

Linked polyaspartate using glycine-serine linker

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre

Plasmid

#243748

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20-Pdp1_NTD-Cre

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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nlsSalicS1

Plasmid

#232190

Purpose

Novel FRET-based biosensor for salicylic acid

Depositor

Alexander Jones

Article

Tang et al Science. 2025 Oct 9;390(6769):188-194. d

Insert

nlsSalicS1

Tags

His-tag

Expression

Plant

Promoter

p16

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-wGBD

Plasmid

#26734

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

GFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pC1-SypHer3s-IMS

Plasmid

#108120

Purpose

Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s-IMS

Tags

ratiometricYFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCold TDP43 WT

Plasmid

#27451

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa, GST, and His6

Expression

Bacterial

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-MYOD1-WT

Plasmid

#194570

Purpose

Mammalian Expression of mEGFP-MYOD1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

MYOD1 (MYOD1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBMCL1

Plasmid

#178203

Purpose

An improved vector for BacMam system

Depositor

Lei Chen

Article

Guo et al Biochem Biophys Res Commun. 2022 Feb 26;

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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cEF.tk-GFP

Plasmid

#33308

Depositor

Linzhao Cheng

Article

Pomper et al Cell Res. 2009 Mar;19(3):370-9.

Insert

HSV-TK/GFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-zeo

Plasmid

#160091

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVie-NcanGFP

Plasmid

#235983

Purpose

Expresses the hyaluronan-binding domain of Neurocan fused to eGFP under the CMV promoter. It contains a sequence for secretory pathway, so the probe is exported to the extracellular space

Depositor

Federico Soria

Article

Fernández-Ballester et al bioRxiv 2025.01.31.635882; doi: 10.1101/

Insert

Neurocan-GFP

Use

AAV

Tags

GFP

Promoter

CMVie

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB

Plasmid

#162572

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB

Expression

Bacterial

Promoter

pBAD

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐hsRNASEH2BCA

Plasmid

#108692

Purpose

For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Inserts

RNASEH2B (RNASEH2B Human)
RNASEH2C (RNASEH2C Human)
RNASEH2A (RNASEH2A Human)

Tags

GST

Expression

Bacterial

Mutation

Shine Dalgarno sequence upstream of start codon a…

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-CytoTape-vivo-HA

Plasmid

#250670

Purpose

CytoTape-vivo structural monomer for CytoTape-vivo fiber formation and elongation for mouse brain in vivo applications

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-vivo-HA

Use

AAV

Expression

Mammalian

Promoter

UbC promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4 p50

Plasmid

#21965

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NF-kB p50 subunit (NFKB1 Human)

Expression

Mammalian

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pSLC-217

Plasmid

#73163

Purpose

Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-relE (relE E. coli (bacteria))

Expression

Bacterial

Promoter

PrhaB

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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