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161,838 results

Showing: 9601 - 9640 of 161838 results

  1. pCR3.1-mutLuc

    Plasmid
    #106458
    Purpose
    luciferase reporter, CMV promoter (3 NFkB sites mutated)
    Depositor
    Georg Weber
    Article
    He et al Biochem Biophys Res Commun. 2004 Aug 13;
    Insert
    luciferase reporter, CMV promoter (3 NFkB sites mutated)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    3 NFkB sites mutated
    Promoter
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    106458-plasmid-map-sequence-id-207856

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  6. Td-CGBE

    Plasmid
    #196599
    Purpose
    Mammalian expression of Td-CGBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    Use
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196599-plasmid-map-sequence-id-386792

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  9. pRPA32-mCherry

    Plasmid
    #211384
    Purpose
    mammalian expression of human RPA32 C-terminally fused to mCherry
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211384-plasmid-map-sequence-id-420404

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  15. pHB-3

    Plasmid
    #140956
    Purpose
    For PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140956-plasmid-map-sequence-id-334291

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  19. HTR1B-Tango

    Plasmid
    #66405
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR1B (HTR1B Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66405-plasmid-map-sequence-id-347879

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  21. pAIP4_3KT9

    Plasmid
    #234192
    Purpose
    Heterologous protein expression of aprataxin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3KT9 (APTX Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234192-plasmid-map-sequence-id-464157

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  22. mTagRFP-T-VASP-5

    Plasmid
    #58027
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    VASP
    Use
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    July 31, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58027-plasmid-map-sequence-id-198671

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  26. MSGV Mouse Acceptor

    Plasmid
    #128176
    Purpose
    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
    Depositor
    David Ott
    Article
    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    MSCV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128176-plasmid-map-sequence-id-252503

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  27. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    Douglas Kim, GENIE Project
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100833-plasmid-map-sequence-id-198271

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  30. PCDH-METTL16-3*Flag

    Plasmid
    #196207
    Purpose
    overexpress METTL16
    Depositor
    Jianjun Chen
    Insert
    WT METTL16 (METTL16 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    NO
    Promoter
    Available since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196207-plasmid-map-sequence-id-383845

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  31. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Boris Fehse
    Article
    Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Use
    Tags
    Expression
    Mutation
    changed Asp at position 249 + 250 to Val
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    60488-plasmid-map-sequence-id-96845

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  35. pAAV-CAG-DIO-ChR2(H134R)-eYFP

    Plasmid
    #127090
    Purpose
    Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation
    Depositor
    Viviana Gradinaru
    Article
    Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:
    Has Service
    AAV PHP.eB
    Insert
    Channelrhodopsin-2
    Use
    AAV
    Tags
    eYFP (C terminal on insert)
    Expression
    Mammalian
    Mutation
    Humanized ChR2 gene with histidine 134 changed to…
    Promoter
    CAG
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127090-plasmid-map-sequence-id-246739

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  36. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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Showing: 9601 - 9640 of 161838 results