164,129 results

1. pGWB401

   Plasmid

   #74795

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA NIA TLS1

   Plasmid

   #196980

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS1 tags
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCre

   Plasmid

   #236212

   Purpose

   Arabinose-inducible Cre recombinase

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   Cre recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. BCJJ345

   Plasmid

   #117504

   Purpose

   YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtYAO

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE smFP-V5 donor (all-in-one) ORF2

   Plasmid

   #200388

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. EC70203

   Plasmid

   #209464

   Purpose

   Cloning protospacers for Cas9 editing

   Depositor

   Cristobal Uauy, Wendy Harwood

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Insert

   Cas9 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAP03-acc(3)IV

   Plasmid

   #69862

   Purpose

   pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

   Depositor

   Bradley Moore

   Article

   Tang et al ACS Chem Biol. 2015 Oct 21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-HAPLN1

   Plasmid

   #200777

   Purpose

   Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Expression

   Mutation

   Promoter

   Synapsin

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-LaminA-C-18

   Plasmid

   #55068

   Purpose

   Localization: Nuclear Envelope, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Lamin A (LMNA Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFJ782 - HygroR

    Plasmid

    #190933

    Purpose

    Hygromycin resistance co-injection marker

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    Hygromycin resistance gene
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT-WT-Myc

    Plasmid

    #177717

    Purpose

    ER protein alpha-1-antitrypsin (WT), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIRES Centrin1 GFP Pur

    Plasmid

    #64341

    Purpose

    Bicistronic expression of Centrin-GFP fusion and Puro resistance gene

    Depositor

    Matthieu Piel

    Insert

    Centrin1 (CETN1 Human)
    

    Use

    Tags

    GFP and IRES-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-EF1a-iFlpV

    Plasmid

    #140137

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iFlpV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307-DPP4-G418

    Plasmid

    #158453

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFP-hPMLIII

    Plasmid

    #181903

    Purpose

    Fluorescently tagged PMLIII

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    hPMLIII (PML Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPT107

    Plasmid

    #85525

    Purpose

    Repressilator with triple reporter

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Inserts

    mCfp
    mVenus
    tetR
    lacI
    cI
    mKate2
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTW335h3

    Plasmid

    #237326

    Purpose

    expresses O. sativa RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    O. sativa RbcL
    O. sativa RbcS-6xHis
    

    Use

    Tags

    6xHis and None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW386c

    Plasmid

    #237321

    Purpose

    expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Insert

    O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe puro HA PIK3CA H1047R

    Plasmid

    #12524

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA H1047R (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H1047R. Tumor-derived allele.

    Promoter

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. hMEF2A WT

    Plasmid

    #118354

    Purpose

    This plasmid expresses human MEF2A-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hMEF2A (MEF2A Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. CMV-Nme2ABE8e

    Plasmid

    #189928

    Purpose

    Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    Nme2ABE8e
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEDA5_GFPmut3_Y66H

    Plasmid

    #80085

    Purpose

    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

    Insert

    GFPmut3_Y66H
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    changed Tyrosine 66 to Histidine

    Promoter

    proB

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-RT003-GPM

    Plasmid

    #163383

    Purpose

    To express sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    GFP-PDGFRtm-2A-mCherry (GPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmiRFP680-N1

    Plasmid

    #136557

    Purpose

    A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    miRFP680
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. sUPRa

    Plasmid

    #223242

    Purpose

    sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

    Depositor

    Colin Akerman

    Article

    Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

    Insert

    sUPRa
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Custom

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. GFP-UBR5

    Plasmid

    #52050

    Purpose

    CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV.hSyn.iGluSnFR3.v857.SGZ

    Plasmid

    #178330

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn iGluSnFR3 v857.SGZ
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pZFN-AAVS1-KKR

    Plasmid

    #159298

    Purpose

    ZFN (right) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYSEAP

    Plasmid

    #37326

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

    Insert

    SEAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. epB_CAG_TetRFlag-nls-GFPx2_DEx2

    Plasmid

    #119910

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-GFPx2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHAGE2 mCherry-Atl3

    Plasmid

    #86680

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL3 (ATL3 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. hSOD1-FLAG

    Plasmid

    #182922

    Purpose

    Mammalian expression plasmid for human WT SOD1

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    [Cu-Zn] Superoxide dismutase 1 (SOD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pZFN-AAVS1_ELD

    Plasmid

    #159297

    Purpose

    ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge