179,521 results
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Plasmid#111209Purposefluorescent fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTNFRSF1A (TNFRSF1A Human)
TagsEYFPExpressionMammalianAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
plenti-CMV-mCherry-T2A-GFP
Plasmid#109427PurposeA lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.DepositorInsertmCherry T2A GFP
UseLentiviralPromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Cas9-P2A-Puro
Plasmid#110837PurposeLentiviral vector for expression of Cas9 in mammalian cells (codon optimized)DepositorInsertCas9
UseLentiviralMutationWTPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate2-P2A-APEX2-csGBP
Plasmid#108875PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmKate2-P2A-APEX2-csGBP
ExpressionMammalianAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUS-FusionRed-PixD
Plasmid#111503PurposeFor PixELL systemDepositorAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.18 CMV-Luc
Plasmid#100984PurposeCMV luciferase reporter - firefly luciferase driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV
ExpressionMammalianAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WT Smarca4-V5 IRES-puro
Plasmid#107058PurposeExpression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsIRES-puroR and V5ExpressionMammalianMutationwild-typePromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#106435PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAG)20-P2A-RFP
Plasmid#105689PurposeDouble fluorescent stalling reporter K(AAG)20DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsP2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCV-hygro-STING V155M
Plasmid#102599PurposeExpress STING V155M for IFN-beta Luciferase reporter assay.DepositorAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTO_CHMP4B_LAP
Plasmid#101850PurposeTet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integrationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCharged Multivesicular Body Protein 4B (CHMP4B Human)
UseGateway cloning, flp-frt recombination, tet-induc…TagsLocalization and Affinity Purification (LAP) tagExpressionBacterial and MammalianPromoterCMVAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_Gal4UAS_Blinatumomab_PGK_mCherry
Plasmid#85437Purpose[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expressionDepositorInsertBlinatumomab
UseLentiviralExpressionMammalianAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAD-GamS
Plasmid#99246PurposeExpresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.DepositorInsertgamS (with C-terminal 6xHis tag)
Tags6xHis tagExpressionBacterialPromoterT7lacAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-teLuc c-myc
Plasmid#100026PurposeEnhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertteLuc, a NanoLuc mutant
Tagsc-mycExpressionMammalianMutationD19S, D85N, C164HPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-Rab11-WT
Plasmid#101047PurposeExpresses HA-tagged human Rab11-WT in mammalian cellsDepositorAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DNA-PKcs PQR>ala
Plasmid#83319Purposehuman DNA-PKcs PQR>alaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DNA-PKcs (PRKDC Human)
ExpressionMammalianMutation5 phosphorylation sites in the PQR cluster substi…Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pirespuro2-CTLA4-mEos2
Plasmid#98285Purposemammalian expression of CTLA4-mEos2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCTLA4 (CTLA4 Human)
TagsmEos2ExpressionMammalianPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting
Plasmid#92141PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mApple-Sec61b-C1
Plasmid#90993PurposeExpresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmApple-Sec61b (SEC61B Human, Synthetic)
TagsmApple (red fluorescence)ExpressionMammalianPromoterCMVAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
piggyBac CMV-GFP-FRT
Plasmid#92078Purposeproduction of transgenic animal and recombination using flipaseDepositorInsertCMV-eGFP and TK-Neo between FRT3 and FRT
UsePiggybacExpressionMammalianPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAIP hIL2 co
Plasmid#90513PurposeLentiviral vector for expression of human codon optimized IL2 with puromycin resistance.DepositorAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
IL2Rα-EGFP
Plasmid#86055PurposeA mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInterleukin-2 receptor subunit alpha (IL2RA Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_PIK3CA_WT
Plasmid#81736PurposeGateway Donor vector containing PIK3CA , part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_PIK3CA_p.E545K
Plasmid#82881PurposeGateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1
Plasmid#87829PurposeHigh-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsMTS
NLS
PTS1
ExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAHYB-GFP
Plasmid#85074PurposeExpression of GFP in Pichia pastorisDepositorPublicationInsertGFP
ExpressionYeastPromoterAOX1Available sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
TagstagRFPExpressionInsectPromoterGal4-UASAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSkylan-NS-N1
Plasmid#86785Purposefor fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NSDepositorTypeEmpty backboneTagsSkylan-NSExpressionMammalianPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMyNT-kan
Plasmid#84693PurposeProtein expression in mycobacteriaDepositorTypeEmpty backboneTagsHis6ExpressionBacterialPromoterAcetamidaseAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk2
Plasmid#81011PurposeExpression of Zdk2 anchored to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZdk2
Tags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB402-omega
Plasmid#74797PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneExpressionPlantPromoter2xCaMV35S enhancer and omegaAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHRPb-NLG (Nlgn1 Mouse, Synthetic)
TagsV5 tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-NSF
Plasmid#74924PurposeMammalian expression of human NSF full-length (744 aa)DepositorAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEX-oG-WPRE-SV40pA
Plasmid#74292PurposepAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAP2
Plasmid#73211Purposeexpress Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)DepositorInsertAAP2
UseAAVExpressionMammalianPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmGFP11C-Tau
Plasmid#71434PurposeSplit GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTau (0N4R) (MAPT Human)
TagsGFP (213-228)ExpressionMammalianAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by