19,773 results

Showing: 7231 - 7260 of 19773 results

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pZW6 (CK2alpha)

Plasmid

#27086

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-ICUE3

Plasmid

#61623

Purpose

Plasma membrane-targeted FRET biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

Lyn-ICUE3 (RAPGEF3 Human)

Tags

ECFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)

Use

Human homologous recombination donor

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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ph3b

Plasmid

#32110

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

BKV VP3

Expression

Mammalian

Mutation

codon modified

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP1-Flag-His-WT

Plasmid

#111572

Purpose

Expresses full length PARP1 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2

Plasmid

#135504

Purpose

Mammalian expression of myc-tagged HLA-A*02:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-V5-KDM3A

Plasmid

#82331

Purpose

lentiviral expression of KDM3A

Depositor

Luke Gaughan

Article

Wade et al Nucleic Acids Res. 2015 Jan;43(1):196-20

Insert

KDM3A (KDM3A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

two silent mutations to make it siRNA resistant

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only
HT PTP-RN

Plasmid

#24056

Depositor

Frederick Stanley

Insert

PTPRN (PTPRN Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pOpen-SP6rpol

Plasmid

#165516

Purpose

DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

SP6 RNA Polymerase

Use

Synthetic Biology

Available Since

July 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Human SERCA2b (pcDNA3.1+)

Plasmid

#75188

Purpose

Mammalian expression of ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSCMV:tdTomato

Plasmid

#30530

Purpose

mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

Has Service

Cloning Grade DNA

Insert

tdTomato

Expression

Mammalian

Mutation

R415H

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hUbC-dCas9-MQ1(Q147L)-EGFP

Plasmid

#89793

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

Q147L, CAA-->CTA

Promoter

hUbC

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pIB184-Km

Plasmid

#90195

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Cre

Plasmid

#198281

Purpose

Expresses Cre in mammalian cells

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Cre

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
secretedBirA-8his

Plasmid

#32408

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA

Tags

8 His tag

Expression

Mammalian

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
NES-EGFP-PH-ARNO2G-I303Ex2

Plasmid

#116868

Purpose

PIP3 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

CYTH2 (CYTH2 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amind acids 252-399, isoleucine 303 changed to gl…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiMPH v2 (Neo)

Plasmid

#92065

Purpose

Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Insert

MS2-P65-HSF1_2A_Neo (HSF1 Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1a

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasKP-hph

Plasmid

#117232

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA/Myc TCF4

Plasmid

#16512

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge

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19,773 results