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Plasmid#174615PurposeBacterial expression of mtrC from Shewanella oneidensis MR-1.DepositorInsertMtrC
UseSynthetic Biology; Shuttle vector in e. coli and …TagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAMP-CY1_NF1 T2 (+minintr, KDR)
Plasmid#119201Purposetransfer vectorDepositorInsertNF1 type 2 mini-gene (NF1 Human)
UseTransfer vectorTagsExpressionMutationPromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-GST-YAP-S127A
Plasmid#33053DepositorInsertYAP (YAP1 Human)
UseTagsGSTExpressionMammalianMutationS127APromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130 Y242A
Plasmid#32823DepositorInsertAMOT p130 (AMOT Human)
UseTagsHAExpressionMammalianMutationE5D, Y242APromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-RPB1_BSD_FKBPF36V_EYFP
Plasmid#163884PurposeAcute Depletion of RBP1-EYFPDepositorInsertRbp1 (Polr2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
NbALFA-TEV219
Plasmid#201487PurposeTANGO detection of V5-tagged proteinDepositorInsertNbALFA-TEV219
UseTagsTEV219ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORF1
Plasmid#206025PurposeAn expression vector for mouse LINE1 ORF1DepositorInsertORF1 derived from mouse LINE1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pRK5-mWnt9b
Plasmid#42287DepositorInsertWnt9b (Wnt9b Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pV1435
Plasmid#111439PurposeSolo vector pV1382 + sgCgADE2DepositorInsertCaCas9/sgCgADE2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse dbx-1 [TJ#11]
Plasmid#16269DepositorInsertdbx1 3'UTR (Dbx1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad3 (EPSM)
Plasmid#14963DepositorInsertSmad3 (EPSM) (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationT178V, S203A, S207A, S212APromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pExo1
Plasmid#53357PurposeExpresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.DepositorInsertsBlaMs
hlyAs
UseTagsExpressionMutationPromoterp(lac/ara-1)Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol-Promoter:NLS-tdMCP-eGFP
Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PECAM-eGFP-Hygro
Plasmid#14686DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsEGFP and PGK-HygroExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
SNAP-EF(N)-10nmSAH-GFP(1-10)
Plasmid#61660Purpose10nm SAH scaffold with EF Hand and split GFP attachment sitesDepositorInsertSNAP-EF(N)-10nmSAH-GFP(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NbV5-TEV219
Plasmid#201481PurposeTANGO detection of V5-tagged proteinDepositorInsertNbV5-TEV219
UseTagsTEV219ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB401NL3F10H
Plasmid#141285PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P2_crU626
Plasmid#191777PurposeCas12a guide expression in dicotsDepositorInsertLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P7_crU626
Plasmid#191782PurposeCas12a guide expression in dicotsDepositorInsertLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
3265 pBS Mcl-1
Plasmid#8763DepositorInsertMcl-1 (Mcl1 Mouse)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
DLC1A
Plasmid#25333PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertDLC1:S1074-L1283 (DLC1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids S1074-L1283PromoterAvailable sinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4-NET-myc
Plasmid#107458PurposeMammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2DepositorInsertNorepinephrine Transporter (NET) (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-VPS28
Plasmid#34803DepositorInsertvacuolar protein sorting 28 (yeast) (VPS28 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hy_pUbi-p63e eGFP SV40
Plasmid#132650PurposeExpresses eGFP mRNA from the Drosophila Ubi-p63e promoterDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA GCNF
Plasmid#16336DepositorInsertGCNF (Nr6a1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
bbCas9pluspAAA
Plasmid#82581PurposeThe plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNADepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBD21_037
Plasmid#109154PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720DepositorInsertsmRFP
iRFP720
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Sec16A
Plasmid#36156PurposeExpresses Sec16A as a YFP fusionDepositorInsertSec16A (SEC16A Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH3FS-GAL1
Plasmid#85782PurposeN-ICE plasmid pH3FS-GAL1DepositorInsertloxP-HphMX-loxP-GAL1p-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 -5'cMyc-SMCl- S957A
Plasmid#32368DepositorInsertSMC1A (SMC1A Human)
UseTagsc-MycExpressionMammalianMutationS957APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-TPT1
Plasmid#31558DepositorInserttumor protein, translationally-controlled 1 (TPT1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCF730
Plasmid#121668PurposeEF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-chi28TAG
Plasmid#188984PurposeExpress chimadanin with TAG at 28 positionDepositorInsertChimadanin with TAG at 28 position
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL50015
Plasmid#50318PurposePlease note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)DepositorInsertCDS, mNEON (Branchistoma lanceolatum)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pK3F
Plasmid#85770PurposeN-ICE plasmid pK3FDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100 constitutiveAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 5' cMyc- SMCI-S966A
Plasmid#32371DepositorInsertSMC1A (SMC1A Human)
UseTagsc-MycExpressionMammalianMutationS996APromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only