166,041 results

1. pTRETightBI-RY-0

   Plasmid

   #31463

   Depositor

   Phil Sharp

   Article

   Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

   Insert

   eYFP and mCherry
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Jan. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lyn11-CIBN-GFP

   Plasmid

   #79572

   Purpose

   expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   Lyn11
   

   Tags

   CIBN (CRY2-binding domain) and EGFP

   Expression

   Mammalian

   Mutation

   G8R (please see depositor comments below)

   Promoter

   CMV

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-myc-Atg12

   Plasmid

   #24923

   Depositor

   Toren Finkel

   Article

   Lee et al J Biol Chem. 2009 Mar 6. 284(10):6322-8.

   Insert

   Atg12 (ATG12 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE81XN-mGold

   Plasmid

   #218959

   Purpose

   Expresses Hisx6-Tagged mGold in bacteria

   Depositor

   Benjamin Glick

   Insert

   mGold
   

   Tags

   6X-His

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFN18A-HaloTag-Biotin

   Plasmid

   #206039

   Purpose

   HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Insert

   Talin R3-IVVI (Tln1 Mouse)
   

   Tags

   10 x His-AviTag and Halo-Tag

   Expression

   Bacterial

   Mutation

   Threonine 809 to Isoleucine, Threonine 833 to Val…

   Promoter

   T7

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. APOE_pLX307

   Plasmid

   #98317

   Purpose

   Lentiviral expression of APOE

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   APOE (APOE Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pax2

   Plasmid

   #35002

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Paired box protein Pax-2 (Pax2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

   Plasmid

   #191389

   Purpose

   Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(1-100)
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SQT1665

   Plasmid

   #78744

   Purpose

   Human expression plasmid for LbCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

    Plasmid

    #216248

    Purpose

    HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    HDR template for human RANGAP1 (RANGAP1 Human)
    

    Use

    CRISPR and TALEN; Hdr template

    Tags

    miniIAA7.3xFlag.P2A.BSD

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBCPB+

    Plasmid

    #18940

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    attP, lacZ, attB
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVβ-p300.DY-myc

    Plasmid

    #30490

    Depositor

    Tso-Pang Yao

    Insert

    p300 (EP300 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    aspartic acid 1399 converted to tyrosine (Acetyla…

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-hSyn-GRAB_SST1.0

    Plasmid

    #208665

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mOPN-pcDNA

    Plasmid

    #106447

    Purpose

    Mammalian expression of murine osteopontin

    Depositor

    Georg Weber

    Article

    Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

    Insert

    osteopontin (Spp1 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHelper_TetR/pTet-Tnpase (camR)

    Plasmid

    #213910

    Purpose

    ATc-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDonor_RB-TnV2

    Plasmid

    #213907

    Purpose

    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-TurboID-V5-BRSK2_IDG-K

    Plasmid

    #135272

    Purpose

    Gateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TurboID-V5-BRSK2 (BRSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-TurboID

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179119

    Purpose

    Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Ezr sgRNA
    

    Use

    AAV

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-PAK2 binding domain

    Plasmid

    #83951

    Purpose

    FLIM acceptor for mEGFP Rac1

    Depositor

    Ryohei Yasuda

    Article

    Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

    Insert

    Pak2 (PAK2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa60-113 only; S74A F84A

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-AncMEcoGII291

    Plasmid

    #205024

    Purpose

    Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 291
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. tdTomato-TOMM20-N-10

    Plasmid

    #58137

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pLV-SspB-HaloTag-TIAM1-DHPH

    Plasmid

    #176127

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pES002 MBP-GSK3β_S9A-HA-His

    Plasmid

    #196184

    Purpose

    Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Insert

    GSK3B (GSK3B Human)
    

    Tags

    HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pYES2-HTH-HHT2

    Plasmid

    #86482

    Purpose

    yeast expression of HHT2 with His10-TEV-HA tag

    Depositor

    Akash Gunjan

    Article

    Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

    Insert

    HHT2 (HHT2 Budding Yeast)
    

    Tags

    HIS10-TEV-HA (HTH)

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pLV-SspB-HaloTag-ITSN1-DHPH

    Plasmid

    #176128

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pInducer10b-EGFP-KRAS-WT

    Plasmid

    #164935

    Purpose

    Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Promoter

    Ubc Promoter and Ubc promoter

    Available Since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pLV-hCCND1-HA

    Plasmid

    #174154

    Purpose

    Lentiviral vector expressing HA-tagged human cyclin D1

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    hemagglutinin (HA)

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

    Plasmid

    #216247

    Purpose

    Express OsTIR1.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    OsTIR1.F74A
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #194715

    Purpose

    Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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31. pTRE-BI-osTIR1

    Plasmid

    #207840

    Purpose

    Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

    Depositor

    Michaela Mueller-McNicoll

    Article

    Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

    Inserts

    osTIR
    mCherry
    mAID_-VHH-GFP4
    

    Tags

    3X HA-Tag and weak NLS

    Expression

    Mammalian

    Mutation

    All K mutated to R

    Promoter

    TRE3G BI promoter

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FUW-TetO-Nr5a1

    Plasmid

    #41081

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Nr5a1 (Nr5a1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO + minimal CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS40H

    Plasmid

    #35479

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast integrating vector

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-hSyn-GRAB_NPY1.0

    Plasmid

    #208676

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUBQ10-GW

    Plasmid

    #209370

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoter

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGWB405-TIC21-sGFP

    Plasmid

    #213865

    Purpose

    Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

    Insert

    chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)
    

    Expression

    Bacterial and Plant

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGKB

    Plasmid

    #85077

    Purpose

    expression in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

    Plasmid

    #231973

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge