159,332 results

1. CROPseq-multi-EBFP2-NLS (pRW1447)

   Plasmid

   #216219

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIP_RNA2

   Plasmid

   #61260

   Purpose

   Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

   Depositor

   David Wang

   Article

   Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

   Insert

   Orsay RNA2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   heat shock promoter

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACAGW-H2B-PAGFP-AAV

   Plasmid

   #33000

   Depositor

   Massimo Scanziani

   Article

   Lien et al Front Neural Circuits. 2011;5:16. Epub 2

   Insert

   H2B-PAGFP (H2BC21 Aequorea victoria, Human)
   

   Use

   AAV; Adeno associated virus

   Tags

   PAGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDB5319

   Plasmid

   #204829

   Purpose

   An integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

   Depositor

   Li-Lin Du

   Article

   Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

   Inserts

   tetR
   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CMV promoter and enotetSW1 promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmGFP-P2A-K(AAA)20-P2A-RFP

   Plasmid

   #105688

   Purpose

   Double fluorescent stalling reporter K(AAA)20

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K(AAA)20
   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ141A

   Plasmid

   #69290

   Purpose

   Gateway entry vector; single gRNA under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-YY1

   Plasmid

   #169018

   Purpose

   Expression of YY1 driven by CMV promoter

   Depositor

   Claes Wadelius

   Article

   Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

   Insert

   human YY1 (YY1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-yoTagRFP-T-Kan

   Plasmid

   #44906

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP-T
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Tsg101

   Plasmid

   #21505

   Depositor

   James Hurley

   Article

   Lee et al Science. 2008 Oct 24. 322(5901):576-80.

   Insert

   Tsg101 (TSG101 Human)
   

   Use

   Tags

   Cherry*

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNF02-mJuniper

    Plasmid

    #219398

    Purpose

    Constitutive expression of mJuniper in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mJuniper
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSDM177

    Plasmid

    #194476

    Purpose

    pHERD30T-crRNA(Lse)_V3

    Depositor

    Joseph Bondy-Denomy

    Article

    Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

    Insert

    crRNA(Lse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GFP-Actb KI

    Plasmid

    #131479

    Purpose

    Endogenous tagging of βactin: N-terminal (amino acid position: D2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Actb, Actb Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBABE-puro-DN-hTERT

    Plasmid

    #1775

    Depositor

    Bob Weinberg

    Article

    Hahn et al Nat Med 1999 Oct;5(10):1164-70.

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative, 2 base pair mutation, catalyti…

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 Flag BimL

    Plasmid

    #24233

    Depositor

    Roger Davis

    Article

    Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

    Insert

    Bim L (BCL2L11 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. Lenti-FAB(mito-LD)

    Plasmid

    #214421

    Purpose

    Detection of mito-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and RspAN-3XHA-V5

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dynamitin-GFP

    Plasmid

    #51419

    Purpose

    expression of GFP-tagged wild-type dynamitin p50 in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    Dynamitin (DCTN2 Chicken)
    

    Use

    Tags

    GFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-Tog

    Plasmid

    #29480

    Depositor

    Linda Wordeman

    Article

    Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

    Insert

    ch-TOG (CKAP5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K19R, E118G, G254R, K1834R

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRG (Lenti_sgRNA_EFS_GFP)

    Plasmid

    #65656

    Purpose

    Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter-driven sgRNA expression and EFS promo…

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. mIFP-N1

    Plasmid

    #54620

    Purpose

    Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson, Xiaokun Shu

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Type

    Empty backbone

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCBFD2Bad

    Plasmid

    #53574

    Purpose

    Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    cbfd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP‐RNASEH1

    Plasmid

    #108699

    Purpose

    For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH1 (nuclear isoform) (RNASEH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

    Plasmid

    #91719

    Purpose

    AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

    Insert

    mEGFP-P2A-paAIP2(R5AR6A)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII 1.3

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. cpLID-micro

    Plasmid

    #168995

    Purpose

    circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    mCh-sspB-T2A-cpLID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET His6 NusA TEV LIC cloning vector (2N-T)

    Plasmid

    #29709

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-NusA-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CAG-mNeonGreen

    Plasmid

    #99134

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CAGGS-MCS-NLS-3XFLAG-V5

    Plasmid

    #196383

    Purpose

    Empty backbone vector to insert cDNA for strong expression in mammalian cells

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Type

    Empty backbone

    Use

    Tags

    3xFLAG and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid

    #216156

    Purpose

    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MAC3_C_VPS35L

    Plasmid

    #209698

    Purpose

    Expresses MAC3-tagged VPS35L in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

    Insert

    VPS35L (VPS35L Human)
    

    Use

    Tags

    MAC3-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Galphai2-mTurquoise2

    Plasmid

    #69621

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai2-mTurquoise2
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV.GfaABC1D.PI.Lck-GFP.SV40

    Plasmid

    #105598

    Purpose

    AAV expression of membrane bound EGFP from GfaABC1D promoter

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Has Service

    AAV5

    Insert

    Lck-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPBT-PE2-PuroTK-pegRNA_GG

    Plasmid

    #173222

    Purpose

    piggyBac transposon containing both PE2 and a pegRNA cloning site.

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

    Inserts

    PE2
    PuroTK
    pegRNA cloning cassette
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, P2A, and hU6

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85006

    Purpose

    For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mEos3.2-TOMM20-N-10

    Plasmid

    #57483

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

36. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTH100

    Plasmid

    #84458

    Purpose

    pJB38-NWMN29-30 + SarA_P1-sGFP-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-sGFP from pCM29
    

    Use

    Tags

    6xHis (on sfGFP)

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBAMD1-6

    Plasmid

    #61566

    Purpose

    Standardized mini-Tn5 delivery plasmid, gentamicin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR221_SLC3A2

    Plasmid

    #132213

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC3A2 (SLC3A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pCAG-VSVG

    Plasmid

    #35616

    Depositor

    Patrick Salmon, Arthur Nienhuis

    Insert

    VSV G protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge