164,223 results

1. MBP-TSF-TEVcs-ULK1

   Plasmid

   #171416

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ULK1 (ULK1 Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-Sac-Abeta(M1-40)

   Plasmid

   #71876

   Purpose

   Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-40) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PiggyBac-TetO-Ascl1-neomycin

   Plasmid

   #176482

   Purpose

   TetO-Ascl1 in a PiggyBac vector with neomycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV_CAG_Gag-MCP

   Plasmid

   #212637

   Purpose

   Lentivirus vector to express an MLV Gag-MCP fusion protein

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   MLV Gag (UniProt ID: P03332) (gag MoMLV)
   

   Use

   Lentiviral

   Tags

   MS2 Capsid Protein (N55K)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EFIa-iTGB1

   Plasmid

   #115799

   Purpose

   Integrin B1 overexpression

   Depositor

   Joan Massague

   Article

   Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

   Insert

   iTGB1 (ITGB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_DLL1_WT

   Plasmid

   #82108

   Purpose

   Gateway Donor vector containing DLL1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   DLL1 (DLL1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmtb-t7-alpha-bungarotoxin

   Plasmid

   #69542

   Purpose

   For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

   Depositor

   Sean Megason

   Article

   Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Tol2, beta-actin promoter, sp6,t7 for zebrafish

   Tags

   Expression

   Mutation

   Promoter

   beta-actin, sp6, t7

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRES-Puro-MGMT

   Plasmid

   #187654

   Purpose

   Expresses hMGMT together with the puromycin resistance gene via an IRES.

   Depositor

   Robert Sobol

   Article

   Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

   Insert

   MGMT (MGMT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TC374

    Plasmid

    #164886

    Purpose

    Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    rpl28-rtTA(Q)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cerulean

    Plasmid

    #15214

    Depositor

    Dave Piston

    Article

    Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

    Insert

    monomeric Cerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available Since

    June 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. g4s pD47

    Plasmid

    #117448

    Purpose

    Expression of Cyth1

    Depositor

    Richard Kahn

    Insert

    Cyth1 (CYTH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mRuby2-ZO1-C-14

    Plasmid

    #55916

    Purpose

    Localization: Tight Junctions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    ZO1 (TJP1 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

    Plasmid

    #197660

    Purpose

    Expresses GFP under the control of J23100 and BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG_Xph20-SNAPf-CCR5TC

    Plasmid

    #135537

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to SNAPf, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Use

    Tags

    SNAPf

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0090

    Plasmid

    #181954

    Purpose

    Beta catenin reporter M50 Super 8x (TCF/LEF binding sites) TOPFlash with Gluc/Cluc

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    7xTOPFlash
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. dSaCas9-VPR

    Plasmid

    #205251

    Purpose

    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

    Depositor

    Rasmus Bak

    Article

    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

    Insert

    dSaCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-C1-FLAG

    Plasmid

    #46956

    Purpose

    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. dCas-hHDAC3-R265P

    Plasmid

    #103786

    Purpose

    Expresses dCas9 fused to human HDAC3 with R265P mutation

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed arginine 265 to proline

    Promoter

    EF1A

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLX302_FOXA1-V5

    Plasmid

    #70090

    Purpose

    Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLminP_Luc2P_RE23

    Plasmid

    #90365

    Purpose

    ATF4 - Amino acid deprivation gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    AARE

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. His6tev CBP HAT (1324-1700)

    Plasmid

    #99340

    Purpose

    bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
    

    Use

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OPEN Beta2-microglobulin

    Plasmid

    #215657

    Purpose

    E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN Beta2-microglobulin (B2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 31 to Cysteine

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Eps15-pmCherryN1

    Plasmid

    #27696

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Eps15 (EPS15 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. α‐eGFP

    Plasmid

    #160214

    Purpose

    Retroviral construct to express an eGFP-tagged version of the α subunit of AP2

    Depositor

    Sandra Schmid

    Article

    Mino et al Traffic. 2020 Sep;21(9):603-616. doi: 10

    Insert

    α‐eGFP (Ap2a2, Ap2a1 Mouse)
    

    Use

    Retroviral

    Tags

    eGFP at Hinge region

    Expression

    Mutation

    Brain-specific Insert

    Promoter

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. STARR-seq luciferase validation vector_ORI_empty

    Plasmid

    #99297

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAK1

    Plasmid

    #73244

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AAK1 (AAK1 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 47-307

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pT7II-2N4Rtau

    Plasmid

    #177653

    Purpose

    Recombinant protein synthesis of human Tau

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    Tau (2N4R) (MAPT Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-28c(+)-YTHDF3

    Plasmid

    #102276

    Purpose

    For bacterial expression of His-tagged human YTHDF3

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-P3-Alb-mScarlet

    Plasmid

    #183461

    Purpose

    To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    Alb (Alb Mouse)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191797

    Purpose

    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13d
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R239A, H244A, R858A, H863A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ArcLight-A242

    Plasmid

    #36857

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    semian CMV

    Available Since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBAC-lacZ

    Plasmid

    #13422

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TAp63alpha-FLAG

    Plasmid

    #27008

    Depositor

    David Sidransky

    Insert

    TAp63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV5B-Flag-Smurf2 wt

    Plasmid

    #11746

    Depositor

    Jeff Wrana

    Article

    Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

    Insert

    SMURF2 (SMURF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pMIR-FLAG-MLL-AF9

    Plasmid

    #71444

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-AF9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV enAsCas12f-YHAM-sgRNA_v7

    Plasmid

    #208356

    Purpose

    Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-YHAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F48Y/S188H/V232A/E316M

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge