159,258 results

1. pMpGWB236

   Plasmid

   #68627

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

   Plasmid

   #85427

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmGFP-P2A-K(AAA)20-P2A-RFP

   Plasmid

   #105688

   Purpose

   Double fluorescent stalling reporter K(AAA)20

   Depositor

   Ramanujan Hegde

   Article

   Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

   Inserts

   P2A-3xFLAG-VHPbeta
   K(AAA)20
   P2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (from vector)

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCherry-Vinculin-23

   Plasmid

   #55159

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.iGluSnFR3.v857.GPI

   Plasmid

   #178335

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV CAG iGluSnFR3 v857.GPI
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   GPI Anchor, IgK-chain, and Myc epi tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

   Plasmid

   #154827

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   TagBFP
   HygR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCold CL7-Cas9

   Plasmid

   #124890

   Purpose

   obtaining full Cas9 RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGK-DTAG-NTERM-GFP

   Plasmid

   #185775

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-FGFR2c-V5/HIS

   Plasmid

   #201107

   Purpose

   expression of human FGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSICO-eGFP-TNPO3

    Plasmid

    #167590

    Purpose

    Expresses human TNPO3 fused to eGFP

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    eGFP-TNPO3 (TNPO3 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    WT

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSecUAG_UTu1D

    Plasmid

    #233867

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant

    Depositor

    Dieter Söll, Natalie Krahn

    Inserts

    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG) and …

    Promoter

    EM7, araBAD, and native As SelD

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-cytoFLARE1.0-TEV

    Plasmid

    #234517

    Purpose

    Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    EGFP-P2A-CaM-V5-TEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pLAS2w.Phyg-HAVCR1

    Plasmid

    #232669

    Purpose

    expresses HAVCR1 in mammalian cells

    Depositor

    Chen-Yang Shen

    Insert

    HAVCR1 (HAVCR1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-pCAG eGFP T2A SP HALO HAPLN1

    Plasmid

    #228200

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    constitutive CAG promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MZB117

    Plasmid

    #228481

    Purpose

    "Empty" C-terminal HRV-3C cleavable GST tag vector for rapid cloning/DNA Assembly via NheI restriction enzyme site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    GST (glutathione S-transferase)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDM069

    Plasmid

    #216821

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold2 fluorescent protein, includes linker for N-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold2
    

    Use

    Tags

    RIPGLIN and VinnyLinker

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK7187

    Plasmid

    #219761

    Purpose

    MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid

    #216695

    Purpose

    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. DRD1-DuET

    Plasmid

    #213227

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD1 (DRD1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PB-3xCTCF-TetO

    Plasmid

    #213023

    Purpose

    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    3xCTCF-TetO
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p663-UBC-miniTurbo-V5-RIOK1_IDG-K

    Plasmid

    #214464

    Purpose

    Gateway destination clone of RIOK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-RIOK1 (RIOK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CL20_mEGFP_LEF1_TAF8

    Plasmid

    #205846

    Purpose

    Express mEGFP-tagged fusion protein, LEF1_TAF8 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    LEF1_TAF8 (LEF1, TAF8 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.1 LARP7-FLAG 7SK

    Plasmid

    #200733

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

    Depositor

    Claudio Alarcón

    Insert

    LARP7 and 7SK (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-lox-roxSTOProx-tRFP-pA-lox

    Plasmid

    #196888

    Purpose

    Dre-activated Cre-inactivated expression of turbo (t)RFP reporter.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    turbo (t)RFP
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET41c-PhTET2

    Plasmid

    #182428

    Purpose

    Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

    Depositor

    Bruno Franzetti

    Article

    Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

    Insert

    Tetrahedral aminopeptidase TET2 from P. hirokoshii
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DCAF1:PBC020-D05:C246337

    Plasmid

    #183974

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    DCAF1:PBC020-D05:C246337 (DCAF1 Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV and MHHHHHHSSGRENLYFQG

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)

    Plasmid

    #181760

    Purpose

    targeting vector

    Depositor

    Tanya Daigle

    Insert

    nls-tagged EGFP and nls-tagged dTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCS2-XTbx5-EnR

    Plasmid

    #117249

    Purpose

    mRNA synthesis

    Depositor

    Gerald Thomsen

    Article

    Horb et al Development. 1999 Apr;126(8):1739-51.

    Insert

    Tbx5-EnR (tbx5-b Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    chimeric protein with engrailed repressor domain…

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAT218

    Plasmid

    #134886

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-uvsX-H6

    Plasmid

    #163913

    Purpose

    Expresses uvsX for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsX
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 3xFlag-eGFP-Flag-SETX

    Plasmid

    #218838

    Purpose

    Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

    Depositor

    Albert La Spada

    Article

    Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

    Insert

    Senataxin (SETX Human)
    

    Use

    Tags

    Flag and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE

    Plasmid

    #101852

    Purpose

    Direct reprogramming of adult fibroblasts to neurons

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    Ascl1, Brn2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6, PGK

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15-ZmPYK

    Plasmid

    #220230

    Purpose

    Inducible expression of Zymomonas mobilis pyruvate kinase

    Depositor

    Liskin Swint-Kruse, Aron Fenton

    Article

    Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

    Insert

    Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV2-ProC3-Cre/mCherry

    Plasmid

    #126006

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    Cre-2A-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC3

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge