164,826 results

1. p2T-U6-sgCTG

   Plasmid

   #232723

   Purpose

   SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting CAG repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABE-dGFP

   Plasmid

   #226863

   Purpose

   Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A111V

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABE-dGFP-gRNA

   Plasmid

   #226864

   Purpose

   Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-shFMR1-mCherry

   Plasmid

   #222963

   Purpose

   Make lentivirus to knock down human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Guo et al Nat Commun. 2023 Jun 26;14(1):3801. doi:

   Insert

   FMR1 shRNA (FMR1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-VDAC1 [N152B/23R-2b]

   Plasmid

   #222171

   Purpose

   Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody. (VDAC1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ7535 pHR (hU6-crSURF2-EFS-PuroR-WPRE)

   Plasmid

   #214879

   Purpose

   Lentiviral vector encoding RfxCas13d targeting SURF2 guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crSURF2-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBiT3.1-N_DCAF1:1-1507

   Plasmid

   #211028

   Purpose

   Mammalian expression of full-length human DCAF1. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DCAF1:M1-E1507 (DCAF1 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GFP-IRES-hSNCA (A30P)

   Plasmid

   #185720

   Purpose

   AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Changed Ala 30 to Pro

   Promoter

   hSyn

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis

   Plasmid

   #157572

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA5A (SEMA5A Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-roGFP1-iR

    Plasmid

    #83314

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iR
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

    Promoter

    T5

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pNIC-CTHF

    Plasmid

    #26105

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    TEV - His6 - Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. mARGAna (cassette 1)

    Plasmid

    #191341

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. epi-BE4max-NG

    Plasmid

    #135977

    Purpose

    Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pET263-pUC57 24xMS2V7

    Plasmid

    #140705

    Purpose

    MS2V7 cloning

    Depositor

    Evelina Tutucci, Robert Singer

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V7
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    24 MS2 loops V7, high-affinity C-variant, inter-s…

    Promoter

    None

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_TGFBR1_WT

    Plasmid

    #82237

    Purpose

    Gateway Donor vector containing TGFBR1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TGFBR1 (TGFB1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Article

    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available Since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-3

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dCas13-NES-eNAT10

    Plasmid

    #238299

    Purpose

    Expresses cytoplasmic version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    HIV NES (C terminal of dPspCas13b)

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid

    #238300

    Purpose

    Expresses nuclear version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available Since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBAD24-sfGFPx1

    Plasmid

    #51558

    Purpose

    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli

    Depositor

    Francisco Malagon, Sankar Adhya

    Article

    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. PDG459 V3

    Plasmid

    #226958

    Purpose

    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. Flag-HA-USP36

    Plasmid

    #22579

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP36 (USP36 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. KW57_HART_RAC1

    Plasmid

    #207399

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL4.R_TRE_minCMV

    Plasmid

    #211518

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SV40 and minCMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSII-EF-miRFP709-hCdt(1/100)

    Plasmid

    #80007

    Purpose

    hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hCdt1(1/100) (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    miRFP709

    Expression

    Mutation

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIR-CMV-SCN2A-Variant-1-IRES-mScarlet

    Plasmid

    #162279

    Purpose

    Eukaryotic expression of human SCN2A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN2A Variant 1, stabilized (SCN2A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp4551.

    Promoter

    CMV

    Available Since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pmTurquoise2-LAMP1

    Plasmid

    #110948

    Purpose

    Expresses LAMP1 fused to mTurquoise2

    Depositor

    Pablo Rivera-Fuentes

    Article

    Halabi et al Nat Commun. 2019 Mar 15;10(1):1232. doi:

    Insert

    Lysosomal-associated membrane protein 1 (LAMP1 Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ClopHensorN

    Plasmid

    #50758

    Purpose

    Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

    Depositor

    Colin Akerman

    Article

    Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

    Insert

    ClopHensorN
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP is EGFP-T203Y

    Promoter

    CAG

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge