Skip to main content
Addgene
Showing: 10321 - 10360 of 159111 results

  3. pBAD-luxKate

    Plasmid
    #229946
    Purpose
    Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)
    Depositor
    Andy Yeh
    Article
    Chen et al Chem. 2024 Nov 12. doi: 10.1016/j.chempr
    Insert
    luxKate
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229946-plasmid-map-sequence-id-457155

    Enlarge

  4. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182487-plasmid-map-sequence-id-360431

    Enlarge

  6. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

    Enlarge

  12. AbVec2.0-IGHG2

    Plasmid
    #99576
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG2 isotype
    Depositor
    Hedda Wardemann
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    99576-plasmid-map-sequence-id-192590

    Enlarge

  26. pCW57.1_V5_BirA*_FLAG_Nt

    Plasmid
    #194070
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-BirA(R118G)-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    194070-plasmid-map-sequence-id-380711

    Enlarge

  29. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

    Enlarge

  35. DDX20A

    Plasmid
    #42376
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DDX20A (DDX20 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pJRoC30_VP8H

    Plasmid
    #188062
    Purpose
    PROSS-designed versatile peroxidase 8H
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35
    Insert
    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    Use
    Tags
    S. cerevisiae native α-factor prepro-leader signa…
    Expression
    Yeast
    Mutation
    38 PROSS mutations, described in publication
    Promoter
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188062-plasmid-map-sequence-id-370147

    Enlarge

Showing: 10321 - 10360 of 159111 results