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179,521 results

Showing: 10321 - 10360 of 179521 results

  1. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108387-plasmid-map-sequence-id-207183

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  5. pKK-TEV-CyOFP1

    Plasmid
    #105799
    Purpose
    Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-CyOFP1
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105799-plasmid-map-sequence-id-203392

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  9. pDEST14-Cys-SpyCatcher002

    Plasmid
    #102829
    Purpose
    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents
    Depositor
    Mark Howarth
    Publication
    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi
    Insert
    Cys-SpyCatcher002
    Tags
    GSSGS, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Cysteine residue in N-terminal region
    Promoter
    T7
    Available since
    Dec. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102829-plasmid-map-sequence-id-200986

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  13. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101715-plasmid-map-sequence-id-196971

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  14. pGL4-AARE-luc2P-Hygro

    Plasmid
    #101787
    Purpose
    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)
    Depositor
    Seiichi Oyadomari
    Publication
    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xAARE
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101787-plasmid-map-sequence-id-194135

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  19. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Publication
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  28. CMV Marina-T2A-nls-mCherry

    Plasmid
    #74216
    Purpose
    genetically-encoded fluorescent voltage indicator
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    fluorescent protein voltage sensor
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    CMV
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74216-plasmid-map-sequence-id-170988

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  31. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83475-plasmid-map-sequence-id-358352

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  34. pLV_Zika_Flag_NS4A

    Plasmid
    #79636
    Purpose
    Third generation Self-inactivating lentivector expressing NS4A from Zika virus
    Depositor
    Vaithi Arumugaswami
    Insert
    NS4A
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    EF1
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79636-plasmid-map-sequence-id-148804

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  40. pG1AK-sfGFP

    Plasmid
    #71739
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Publication
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    sfGFP
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71739-plasmid-map-sequence-id-130138

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Showing: 10321 - 10360 of 179521 results