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164,425 results

Showing: 10321 - 10360 of 164425 results

  2. pAAV EF1a DIO iChloC 2A dsRed

    Plasmid
    #70762
    Purpose
    An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    E83Q,E90R,E101S,D156N,T159C
    Promoter
    EF1a
    Available Since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    70762-plasmid-map-sequence-id-134333

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  6. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Promoter
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

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  12. pLP-PIGA (pLP140)

    Plasmid
    #168461
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.
    Depositor
    Jef Boeke, Matthew Maurano
    Article
    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    168461-plasmid-map-sequence-id-362577

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  16. pDONR221-MTCH1_STOP

    Plasmid
    #161108
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    MTCH1 (MTCH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161108-plasmid-map-sequence-id-352172

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  17. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135049-plasmid-map-sequence-id-269408

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  29. pETDuet-TFIIF

    Plasmid
    #171082
    Purpose
    Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    RAP30 (GTF2F2 Human)
    RAP74 (GTF2F1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171082-plasmid-map-sequence-id-335551

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  31. M3-PAmcherry1

    Plasmid
    #114189
    Purpose
    studying clustering of M3 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    Use
    Tags
    3xHA and PAmCherry
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114189-plasmid-map-sequence-id-224304

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  35. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid
    #127511
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127511-plasmid-map-sequence-id-250763

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  40. pEGFP-C1-FLAG

    Plasmid
    #46956
    Purpose
    Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46956-plasmid-map-sequence-id-352459

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Showing: 10321 - 10360 of 164425 results