4,146 results

1. VPS13C^mClover3

   Plasmid

   #118760

   Purpose

   VPS13C internally tagged with mClover3 at residue 1914.

   Depositor

   Pietro De Camilli

   Article

   Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

   Insert

   VPS13C (VPS13C Human)
   

   Use

   Tags

   mClover3 inserted at position aa 1914

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Nathan Shaner, Roger Tsien, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-DEST47 PINK1 C-GFP

   Plasmid

   #13316

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLCAR-CD19-CD3z

   Plasmid

   #135993

   Purpose

   Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Scott McComb

   Article

   Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

   Insert

   FMC63-3z-P2A-EGFP (CD247, CD28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT4/HB

   Plasmid

   #108352

   Purpose

   Optimized Sleeping Beauty Transposon Vector with MCS

   Depositor

   Wolfgang Uckert

   Article

   Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE NFkB-TA-LUC-UBC-GFP-W

   Plasmid

   #49343

   Purpose

   pHAGE NFkB-TA-LUC-UBC-GFP-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   eGFP (eGFP )
   Luciferase
   NFkB consensus binding sequence
   TA
   UBC
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-T7pol

   Plasmid

   #59926

   Purpose

   Expression vector for T7 RNA polymerase gene

   Depositor

   Ian Wickersham

   Insert

   T7 RNA polymerase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSGV-1D3-28Z All ITAMs intact

    Plasmid

    #107226

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs intact

    Depositor

    Steven Rosenberg, James Kochenderfer

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z All ITAMs intact
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-shRNA-ctrl

    Plasmid

    #85741

    Purpose

    shRNA ctrl

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA ctrl
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Flag mTOR wt

    Plasmid

    #26603

    Purpose

    Mammalian expression of human flag-tagged mTor

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ER-GCaMP6-150

    Plasmid

    #86918

    Purpose

    Fluorescent reporter for ER calcium signaling

    Depositor

    Timothy Ryan

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    ER-GCaMP6-150
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

    Promoter

    CAMKII

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-MAPPER

    Plasmid

    #117721

    Purpose

    MAPPER Selectively Labels ER-PM Junctions

    Depositor

    Jen Liou

    Article

    Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

    Insert

    MAPPER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. CRY2olig-mCherry

    Plasmid

    #60032

    Purpose

    Expresses a fusion of CRY2PHR E490G with mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2PHR E490G (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E490G

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3 HA-Sumo2 WT

    Plasmid

    #48967

    Purpose

    Expresses HA SUMO2 Wild Type in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Human V5-IRF3-pcDNA3

    Plasmid

    #32713

    Depositor

    Saumen Sarkar

    Article

    Zhu et al J Immunol. 2010 May 15;184(10):5768-76.

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBAD33.1

    Plasmid

    #36267

    Depositor

    Christian Raetz

    Article

    Chung et al Biochemistry. 2010 May 18;49(19):4126-37

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PB-TO-hNGN2

    Plasmid

    #172115

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCFD4-U6:1_U6:3tandemgRNAs

    Plasmid

    #49411

    Purpose

    expression of two gRNAs from Drosophila U6:1 and U6:3

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Inserts

    dU6-1:gRNA
    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1 and dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pcDNA-HDAC6-FLAG

    Plasmid

    #30482

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge