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Plasmid#188011PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insGFP.3
Plasmid#188012PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.1
Plasmid#188019PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.2
Plasmid#188020PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.3
Plasmid#188021PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.1
Plasmid#188022PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.2
Plasmid#188023PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2GFP.3
Plasmid#188024PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryBFP.1
Plasmid#187995PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryBFP.2
Plasmid#187996PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryGFP.1
Plasmid#187998PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-gcryGFP.2
Plasmid#187999PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-cmlc2BFP.1
Plasmid#188001PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-cmlc2BFP.2
Plasmid#188002PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-cmlc2BFP.3
Plasmid#188003PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-cmlc2GFP.3
Plasmid#188006PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker cmlc2 GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-R-insBFP.1
Plasmid#188007PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted mRFP protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_PAC
Plasmid#122564PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Puromycin selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_BSR
Plasmid#122567PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Blasticidin selection)DepositorTypeEmpty backboneUseTags3x hemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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vAS1
Plasmid#198745Purpose3xHA-3'UTRmg105 -pmg153-NATDepositorTypeEmpty backboneUseEndogenous tagging of mimivirus/megavirus genesTagsHA tagExpressionMutationPromoterpmg741Available sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.3
Plasmid#188015PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.1
Plasmid#188013PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.2
Plasmid#188014PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.1
Plasmid#188016PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.2
Plasmid#188017PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryGFP.3
Plasmid#188018PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin GFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-dUG-DH1
Plasmid#138963PurposeUsed to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.DepositorArticleInsertDH1 oligo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3MYC_PAC
Plasmid#122566PurposeVector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)DepositorTypeEmpty backboneUseTags3x c-Myc tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_NEO
Plasmid#122565PurposeVector for endogenously tagging Giardia genes with a triple HA tag (G418 selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Clim Act MCH (CAM)
Plasmid#104443PurposeExpresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope.DepositorInsertmCherry
UseExpression in corallochytrium (single-celled euka…TagsExpressionMutationPromoteractinAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
BirA His-tag pLEMO
Plasmid#119817PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTags6-Histidine-tagExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTagsExpressionBacterialMutationPromoterRhamnose promoterAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
vHB178
Plasmid#193457Purpose3xHA endogenous tagging of pandoravirus/mollivirus genesDepositorInsert3xHA
UsePandoravirus/mollivirus expressionTagsExpressionMutationPromoter----Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
vHB179
Plasmid#193458Purpose3xFLAG endogenous tagging of pandoravirus/mollivirus genesDepositorInsert3xFLAG
UsePandoravirus/mollivirus expressionTagsExpressionMutationPromoter---Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
{Ud}RpL14.Dm
Plasmid#53220Purpose{Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 rescueDepositorInsertRpL14 (RpL14 Fly)
UseAttb drosophila melanogaster transformationTagsExpressionMutation14 synonymous mutations introducedPromoterendogenous promoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6 - iFAST
Plasmid#209414PurposeiFAST containing plasmid for endogenous fusion constructsDepositorInsertiFAST
UseTagsExpressionYeastMutationPromoternoneAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA
Plasmid#191528PurposeVector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabainDepositorInsertSLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS414-ATG41
Plasmid#113080PurposeContain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.DepositorInsertATG41
UseTagsExpressionBacterial and YeastMutationPromoterATG41 endogenous promoterAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
RF-227-UB-BMX
Plasmid#79186PurposeExpression of the C-terminal fragment of BMX that is endogenously generated by caspase cleavage in mammalian cellsDepositorInsertBMX (BMX Human)
UseTags3x Flag and ubiquitinExpressionMammalianMutationdelta 1-242PromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK501
Plasmid#72246PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationGln206 to Arg and changes tryptophan-151 to pheny…PromoterT7 and endogenousAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Gfap- TagIN-HA
Plasmid#196491PurposeTo tag endogenous mouse GFAP with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-aid-p2a-GFP
Plasmid#161976Purposehomologous donor template for endogenous taggingDepositorInsertGSlinker-aid-GFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-GFP-p2a-aid
Plasmid#161975Purposehomologous donor template for endogenous taggingDepositorInsertGFP-aid-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Hyg
Plasmid#236486PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.DepositorInsertmStayGold
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-Nat
Plasmid#236485PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Nat.DepositorInsertmStayGold
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-Nat
Plasmid#236488PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.DepositorInsertmCherry
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-Nat
Plasmid#236494PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.DepositorInsertDendra2
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Hyg
Plasmid#236480PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.DepositorInsertsfGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Nat
Plasmid#236491PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only