We narrowed to 22,833 results for: Sis

1. pMpGE006

   Plasmid

   #108722

   Purpose

   Cas9 expression in M.polymorpha

   Depositor

   Takayuki Kohchi, Shigeo Sugano

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Insert

   Atco-Cas9-Pea3ter
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   MpEFpro

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB508

   Plasmid

   #74850

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONOR-tagBFP-PSM-EGFP

   Plasmid

   #100603

   Purpose

   Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   EGFP-T2A-Puro and tagBFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV300

   Plasmid

   #204968

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

   Plasmid

   #115487

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-Pds1(434)

   Plasmid

   #39847

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   Pds1 (PTTG1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA6-LRRC26-V5-His

   Plasmid

   #127817

   Purpose

   Expression of human LRRC26 in mammalian cells

   Depositor

   Jiusheng Yan

   Article

   Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

   Insert

   LRRC26 (LRRC26 Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG302 22aa SunTag VP64 g4+g17

   Plasmid

   #120252

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVH-EF1A-BRD3R

   Plasmid

   #130696

   Purpose

   Lentiviral plasmids encoding human BRD3R

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   Human BRD3R (BRD3 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVG1

    Plasmid

    #111444

    Purpose

    Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgScADE2/stop-codon repair
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO GFP DNAJB8

    Plasmid

    #19504

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB8 (DNAJB8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO HSPA2

    Plasmid

    #19458

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA2 (HSPA2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiU6-hMYOD1 gRNA_1-MS2-Puro

    Plasmid

    #192673

    Purpose

    Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human MYOD1 activating gRNA #1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroSuper-shFyn

    Plasmid

    #26985

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Fyn (FYN Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pMpGE_En01

    Plasmid

    #71534

    Purpose

    Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Ikuko Hara-Nishimura, Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-AktAR-Kras

    Plasmid

    #123357

    Purpose

    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

    Depositor

    Jin Zhang

    Article

    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

    Insert

    AktAR-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCK081 desmaMCS:minpromCerulean,exorh:mCherry

    Plasmid

    #200014

    Purpose

    Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter mCerulean, exorh:mCherry

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Luc-SIRT1 mutant 3'UTR

    Plasmid

    #20380

    Depositor

    Charles Lowenstein

    Article

    Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

    Insert

    SIRT1 (SIRT1 Human)
    

    Use

    Luciferase

    Mutation

    mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3_CRKL-Y207F-V5

    Plasmid

    #196016

    Purpose

    Expresses CRKL Y207F, V5 tagged

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. drebrin-His

    Plasmid

    #40363

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCDH-CAG-NCreIntN-T2A-mTagBFP2

    Plasmid

    #160500

    Purpose

    Expresses NCreIntN in mammalian cells in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    NCreIntN
    

    Use

    Cre/Lox and Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. B2375_CRY2olig-mEGFP

    Plasmid

    #215109

    Purpose

    Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    CRY2olig-mEGFP (CRY2 Mustard Weed)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    E490G, 1-498

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCS2+PIF6CreN

    Plasmid

    #131780

    Purpose

    Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of the CreLite system

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PIF6CreN
    

    Use

    Cre/Lox; Zebrafish expression

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. GFP-Talin-L432G

    Plasmid

    #26725

    Depositor

    Anna Huttenlocher

    Article

    Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

    Insert

    Talin (Tln1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 432 to Glycine to block ability o…

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pCDNA-3xFLAG-TCAB1

    Plasmid

    #51630

    Purpose

    Mammalian expression of 3xFLAG tagged TCAB1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

    Insert

    TCAB1 (WRAP53 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pAL188

    Plasmid

    #22277

    Purpose

    Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTn7·X-nia

    Plasmid

    #122592

    Purpose

    Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS/Pm->Nia
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDGE657

    Plasmid

    #153255

    Purpose

    M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Tags

    6xHis-GST

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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32. pGEX-4T-1-RIPK3

    Plasmid

    #78827

    Purpose

    To purify GST-tagged recombinant RIPK3 protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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33. pLL3.7-K122-TEAD-p-H2B-mCherry

    Plasmid

    #139648

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    IRES-GFP
    

    Use

    Lentiviral

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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34. pcDNA3.1(+)-mTagBFP2-MRTF-B

    Plasmid

    #182062

    Purpose

    Mammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

    Depositor

    Jin Zhang

    Article

    Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

    Insert

    mTagBFP2-MRTF-B (Mrtfb Mouse)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLPP5

    Plasmid

    #209967

    Purpose

    Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    Promoter (P_sppQ)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. CLPIT Cry2PHR-mCh-RhoA

    Plasmid

    #42959

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-RhoA (RHOA Mustard Weed, Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Tet-Off promoter

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDONOR-tagBFP-PSM-dTOMATO

    Plasmid

    #100604

    Purpose

    Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    dTOMATO-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDUP1

    Plasmid

    #223516

    Purpose

    inactive cogfp under control of Ptet, cogfp under control of Ptac

    Depositor

    Yolanda Schaerli

    Article

    Ljiljana et al eLife13:RP97216

    Inserts

    CoGFP
    CoGFP inactivated
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    anti-dimerization mutations S129G, 22 C154S, D156…

    Promoter

    Ptac, Ptac and Ptet, and Ptet

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAdSh.U6.gRNAGFP

    Plasmid

    #58255

    Purpose

    U6 promoter-driven single guide RNA complementary to eGFP

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAGFP cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid

    #47387

    Purpose

    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    glutamate-gated chloride Channels
    

    Expression

    Mammalian

    Mutation

    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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