We narrowed to 40,651 results for: tro

1. miniSOG-Rab5a-7

   Plasmid

   #57774

   Purpose

   Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   Rab5a
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pRK-Myc-Smurf1 delHECT

   Plasmid

   #13677

   Depositor

   Ying Zhang

   Article

   Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

   Insert

   Smurf1 deltaHect (SMURF1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Smurf1 without HECT domain (deleted amino acids 3…

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMK375 (Hygro-P2A-Stag-3FLAG)

   Plasmid

   #121186

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-Stag-3FLAG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TR-Csy4-H29A

   Plasmid

   #80602

   Purpose

   Expresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-H29A
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   His29 mutated to Ala

   Promoter

   CBA

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAG-mCherry-Otx2shRNA1

   Plasmid

   #73979

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LbCas12a

   Plasmid

   #188493

   Purpose

   Expresses FLAG tagged LbCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LZF39: Cre-off hSyn-CheRiff-CFP (FAS)

   Plasmid

   #104118

   Purpose

   synOptopatch construct

   Depositor

   Adam Cohen

   Article

   Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

   Insert

   CheRiff-CFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin I

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS-puro shNEDD4L #2

   Plasmid

   #27017

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   shRNA NEDD4L #2 (NEDD4L Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pMSCV LDTS-LIRmut

    Plasmid

    #189001

    Purpose

    lipophagy-inducing adaptor protein, negative control

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-LIRmut
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAd-Track SIRT1

    Plasmid

    #8440

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-sgRNA_Dgcr8_1

    Plasmid

    #73525

    Purpose

    Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    DGCR8
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pMSCV-FKBP-empty

    Plasmid

    #221239

    Purpose

    Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    FLAG-4xFKBP scaffold
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-VEGFR2-570

    Plasmid

    #21309

    Depositor

    Donald Ingber

    Article

    Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

    Insert

    VEGFR2 promoter (KDR Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pSEM229 - [Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR]

    Plasmid

    #159896

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | mNeonGreen | cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-104

    Plasmid

    #79842

    Purpose

    Expresses NLS-PpsR2-VP16 under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-VP16
    

    Use

    Tags

    Nuclear localization signal of nuclear cap-bindin…

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)

    Plasmid

    #104116

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    QuasAr3-Citrine
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo

    Plasmid

    #163084

    Purpose

    rTetR DNA binding domain fused to GBP (anti-GFP nanobody)

    Depositor

    Lacra Bintu

    Article

    Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-mPer2ER-pBABEpuro

    Plasmid

    #61430

    Purpose

    Express mPER2-ER* fusion protein

    Depositor

    Aziz Sancar

    Article

    Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

    Insert

    mPER2 (Per2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag and mouse estrogen receptor ligand binding d…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

21. PB-EEA-g1-PGK-Puro

    Plasmid

    #102908

    Purpose

    PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCI-FLAG-mTUT4

    Plasmid

    #60041

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT4

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT4 (Tut4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pFvL118

    Plasmid

    #64752

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 SWS cone opsin

    Plasmid

    #109363

    Purpose

    Human SWS cone opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    SWS cone opsin (OPN1SW Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSuper mouse DGCR8-1

    Plasmid

    #14772

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    DGCR8 shRNA (Dgcr8 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pCDNA-FRT-FAK-kinase30

    Plasmid

    #59124

    Purpose

    FAK fret control sensor without FERM domain with 30 nm linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTW081

    Plasmid

    #64755

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pCDNA3.0_nucLAMA-F98 IRES EGFP

    Plasmid

    #136618

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFAP-Archon-EGFP

    Plasmid

    #187979

    Purpose

    Expresses the fluorescent voltage indicator Archon under the GFAP promoter

    Depositor

    Chris Dulla

    Article

    Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

    Insert

    Archon1-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

    Plasmid

    #189807

    Purpose

    Retroviral delivery of Cas9

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. plentiCRISPR-sgGOT1

    Plasmid

    #72874

    Purpose

    The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgGOT1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

34. plx208-CMV-Gal4DBD-CapN-SsrA-CapC

    Plasmid

    #213533

    Purpose

    Expresses one of the split transcription factor component in mammalian cells

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    SspB-EGFP-VP16
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-FLAG TIF1gamma M (456-887)

    Plasmid

    #15735

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    TIF1 gamma M (TRIM33 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Middle region (amino acids 456-887).

    Promoter

    Available since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRDA_186

    Plasmid

    #133458

    Purpose

    U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPJ23116-MS2-rep

    Plasmid

    #184842

    Purpose

    Expression of the rep protein of phage MS2

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Replicase gene MS2 phage
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23116

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge