We narrowed to 18,127 results for: Mut

1. pEB2-Katushka

   Plasmid

   #104008

   Purpose

   Plasmid encoding Katushka

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Katushka
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA HA FAK Y925F

   Plasmid

   #50509

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y925F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pMH220

   Plasmid

   #122836

   Purpose

   dCas9 with GreenGate B-C flanking sequences

   Depositor

   Markus Schmid

   Article

   Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

   Insert

   Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Contains D10A and H840A mutations

   Promoter

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xHA-BioID_pAS28

   Plasmid

   #118223

   Purpose

   expresses 3xHA-tagged BioID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   BioID (BirA mutant)
   

   Use

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   R118G

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. L304-EGFP-Tubulin-K40R

   Plasmid

   #64059

   Purpose

   EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

   Insert

   tubulin
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5E-mcs2

   Plasmid

   #138362

   Purpose

   Gateway compatible 5' entry clone contains mutli-cloning sites sequence2.

   Depositor

   Nathan Lawson

   Article

   Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

   Insert

   sequence multi cloning sites
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-Fv2E-flt4um200-ns

   Plasmid

   #67696

   Purpose

   middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   Fv2E-flt4um200
   

   Use

   Tags

   HA

   Expression

   Mutation

   um200, no stop codon; N1056S

   Promoter

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. peCALWY-NB

   Plasmid

   #22239

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY non-binding variant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C284S, C287S, C413S and C416S mutations linker c…

   Promoter

   Available Since

   Jan. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWP78-PVCpnf_pvc13-Ad5DL491-492

    Plasmid

    #198290

    Purpose

    Produces PVCs retargeted with non-binding mutant of Ad5 knob

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-Ad5DL491-492
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-EGFP-DmFMR1_B

    Plasmid

    #145920

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (T214I and N501S) and th…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-p40R57Q PX

    Plasmid

    #19009

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa1-148 (PX domains) site directed mutagenesis: …

    Promoter

    Available Since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Vh-HA-3xFRB

    Plasmid

    #20223

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    One copy of FRB has a T2098L mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB1-moxVenus

    Plasmid

    #103993

    Purpose

    Plasmid encoding moxVenus

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxVenus
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-L1-PGK-TKBsd-L2

    Plasmid

    #141009

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKBsd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEB1-mClover3

    Plasmid

    #103994

    Purpose

    Plasmid encoding mClover3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mClover3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNR291

    Plasmid

    #79555

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    GFPmut3b
    mTagBFP
    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pICH51266

    Plasmid

    #50267

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (1.3 kb ), 35s (Cauliflower Mosaic Virus) + 5'UTR omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS-lpp0.2-H38A

    Plasmid

    #201788

    Purpose

    mutant of PadR; transcriptional regulator can respond to p-coumaric acid

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    PadR (padR Bacillus subtilis)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    H38A

    Promoter

    Available Since

    July 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pICSL70008

    Plasmid

    #50336

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MAC_N_TBR1

    Plasmid

    #167803

    Purpose

    Expresses TBR1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    TBR1 (TBR1 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pReporter_5

    Plasmid

    #60566

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p413TEF-Sir2-H364Y

    Plasmid

    #51743

    Purpose

    overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H364Y

    Promoter

    TEF1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWA PAS-E BAD K-GAFm

    Plasmid

    #39866

    Depositor

    Vladislav Verkhusha

    Article

    Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

    Inserts

    iRFP protein PAS domain
    iRFP protein GAF domain (mutated)
    

    Use

    Tags

    E coil and K coil

    Expression

    Bacterial

    Mutation

    F165Y, D232Y, W308R

    Promoter

    arabinose inducible and rhamnose inducible

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-HA-MAVS-M142A(KaganF61)

    Plasmid

    #51999

    Purpose

    Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Met 142 to Ala

    Promoter

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

29. MAC_N_SOX15

    Plasmid

    #167792

    Purpose

    Expresses SOX15 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    SOX15 (SOX15 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUG-248

    Plasmid

    #104709

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    PUB22 C13A (PUB22 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    C13A, BpiI/BsaI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUG-21

    Plasmid

    #104707

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    PUB22 WT (PUB22 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1_PA-Cre-Y324F

    Plasmid

    #122961

    Purpose

    Catalytically inactive mutant of PA-Cre

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre-Y324F
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK_proB_eGFP

    Plasmid

    #59845

    Purpose

    Constitutively expresses eGFP with proB promoter

    Depositor

    Timothy Whitehead

    Article

    Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    S2R,S65G,S72A

    Promoter

    proB

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCAG APX W41F dimeric (mito matrix)

    Plasmid

    #40307

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    Flag

    Expression

    Mutation

    W41F mutation.

    Promoter

    CAG

    Available Since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EC2_7_dCas9_HC_sgRNA

    Plasmid

    #163711

    Purpose

    High copy plasmid with dCas9 and sgRNA expression cassettes

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLNHA-C1-HsTAB182_K

    Plasmid

    #146774

    Purpose

    Mammalian Expression of HsTAB182

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsTAB182 (TNKS1BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    2 silent mutations compared to the sequence given…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET15bLCFPT7

    Plasmid

    #53545

    Purpose

    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

    Depositor

    Lingchong You

    Article

    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

    Insert

    T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJL-mGold

    Plasmid

    #157997

    Purpose

    Expression of mGold in yeast cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    mGold is mVenus with L46F;T63S mutations

    Promoter

    pTDH(GAP promoter)

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pICSL70004

    Plasmid

    #50334

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAC-EsaR-D91G

    Plasmid

    #47646

    Purpose

    pAC-EsaR with a a272g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only