We narrowed to 24,787 results for: nes

1. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #101061

   Purpose

   AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2-F391W
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

   Plasmid

   #173870

   Purpose

   Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

   Plasmid

   #127355

   Purpose

   gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and mVenus

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

   Plasmid

   #173873

   Purpose

   Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3- myc NES-mCE(K294A) NLS

   Plasmid

   #82467

   Purpose

   Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NLS deleted from mouse Capping Enzyme (Rngtt Mouse)
   

   Expression

   Mammalian

   Mutation

   deletion of KRKY from 573-657

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

   Plasmid

   #172510

   Purpose

   Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - dead huDisCas7-11 - NES - huADAR2
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

   Plasmid

   #186046

   Purpose

   AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   NES-jGCaMP8s
   

   Use

   AAV

   Tags

   NES-6xHis

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173869

   Purpose

   Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-MCS-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

   Plasmid

   #186045

   Purpose

   AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   NES-jGCaMP8m
   

   Use

   AAV

   Tags

   NES-6xHis

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid

    #158162

    Purpose

    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    2xV5-TurboID-NES
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-miRFP680-NES-p2A-dTomato-2xrGBD

    Plasmid

    #222638

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    miRFP680-NES-p2A-dTomato-2xrGBD
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

    Plasmid

    #182824

    Purpose

    Cre-dependent expression of cytosolic APEX

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    APEX.NES-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173874

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid

    #60492

    Purpose

    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm

    Depositor

    Dorus Gadella

    Article

    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

    Inserts

    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    

    Tags

    SGFP2 and SGFP2(T65G)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTransgenesis p3 sv40 minimal promoter:: Katushka RFP

    Plasmid

    #131731

    Purpose

    Transposon based transgenesis

    Depositor

    Rene Maehr

    Article

    Stanney W et al Dev Biol. 2020 Mar 15;459(2):161-180. do

    Insert

    SV40MinPromoter
    

    Use

    Xenopus expression

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-FLEX-NES-jGCaMP8f-WPRE

    Plasmid

    #186044

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8f under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8f
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173868

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax

    Plasmid

    #173867

    Purpose

    Mammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge