We narrowed to 17,654 results for: She

1. pDEST47-CYFIP1.GFP

   Plasmid

   #109139

   Purpose

   GFP-tagged CYFIP1 expression construct

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. mP58.FL1-pCDNA3

   Plasmid

   #21883

   Depositor

   David Ron

   Article

   Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

   Insert

   P58 (Dnajc3 Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv9_CBX1_2xChromo-13XL-BASU

   Plasmid

   #179412

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1.2xChromo-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2 Blast CRLF2

   Plasmid

   #194090

   Purpose

   Expression of human CRLF2 gene

   Depositor

   Martin Carroll

   Article

   Hurtz et al J Clin Invest. 2020 Jul 1;130(7):3637-36

   Insert

   CRLF2 (CRLF2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLJM60-FLAG-SLC38A9.1

   Plasmid

   #71858

   Purpose

   lentiviral stable expression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. XE248 pCDNA3.1+zeo-VSV-KLHL12

   Plasmid

   #16761

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)
   

   Tags

   VSV

   Expression

   Mammalian

   Available Since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_MAX

   Plasmid

   #234044

   Purpose

   Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   MAX (MAX Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-C1483F

   Plasmid

   #69008

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-C1483F (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Cysteine 1483 to Phenylalanine

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-Ef1a-fDIO-ChrimsonR-tdTomato

   Plasmid

   #171027

   Purpose

   Expresses ChrimsonR-tdTomato fusion after Flp-dependent recombination

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1 and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   ChrimsonR is fused to tdTomato

   Promoter

   Ef1a

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GANLS

    Plasmid

    #50852

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. RANLS

    Plasmid

    #50843

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZ699

    Plasmid

    #208117

    Purpose

    Payload yeast assembly vector with TK-mActb on backbone

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    Synthetic Biology; Copy number inducible

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    Human PGK1 for thymidine kinase

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mH33B-mClover3

    Plasmid

    #171496

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    H3.3B (H3f3b Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-GRK3

    Plasmid

    #32689

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK3 (GRK3 Bovine)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIN-Ldha

    Plasmid

    #175939

    Purpose

    knockout mouse Ldha

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Ldha gRNA (Ldha Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHiUGE TurboID-HA donor (all-in-one) ORF2

    Plasmid

    #200385

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

    Plasmid

    #224490

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-iRFP
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBABE-HA-GOLPH3

    Plasmid

    #21687

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Golgi Phosphoprotein 3 (GOLPH3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDFDuet-nsp7-nsp8

    Plasmid

    #159092

    Purpose

    Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

    Depositor

    Seth Darst

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Inserts

    NSP7
    NSP8
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDEST-FRT/T0-Flag-BRCA1

    Plasmid

    #71117

    Purpose

    mammalian expression of Flag-BRCA1 siRNA resistant construct

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV/TO

    Available Since

    Nov. 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pHiUGE TurboID-HA donor (all-in-one) ORF1

    Plasmid

    #200384

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. AAV_Cdx2 HMEJ donor

    Plasmid

    #97319

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Cdx2 HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pEGFP-ATF6-(S1P-)

    Plasmid

    #32956

    Purpose

    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1Protease site mutation: R415A/R416A

    Promoter

    CMV

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV4Neo-murine IL-17RA-HA

    Plasmid

    #46859

    Purpose

    expresses mouse IL-17RA

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA (Il17ra Mouse)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pAAV-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35516

    Purpose

    AAV expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    Arch3.0-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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30. PB-XL-AncBE4max

    Plasmid

    #136254

    Purpose

    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    AncBE4max_GFP
    

    Use

    CRISPR; Piggybac dna transposon

    Expression

    Mammalian

    Mutation

    The AncBE4max_GFP insert was PCR amplified from a…

    Promoter

    TRE3G

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMT-C34-H

    Plasmid

    #223966

    Purpose

    Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

    Depositor

    Dmitriy Mazurov

    Article

    Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

    Insert

    Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK5 bovine GRK5

    Plasmid

    #14690

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK5 (GRK5 Bovine)
    

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

33. pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

    Plasmid

    #229828

    Purpose

    A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCGN-ATF6 (1-373)m1

    Plasmid

    #27174

    Purpose

    For mammalian expression of the cytoplasmic domain of human ATF6 with mutations eliminating DNA binding and with an HA tag.

    Depositor

    Ron Prywes

    Article

    Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only aa 1-373 Amino acids 315–317 are c…

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pJET-CMV-GFP-P2A-K20-P2A-mCherry

    Plasmid

    #185618

    Purpose

    Ribosomal stalling reporter: positive control

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-K20(AAA)-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJET-CMV-GFP-P2A-K0-P2A-mCherry

    Plasmid

    #185619

    Purpose

    Ribosomal stalling reporter: empty

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-empty-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJET-CMV-GFP-P2A-I15-P2A-mCherry

    Plasmid

    #185620

    Purpose

    Ribosomal stalling reporter: 15x isoleucine

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    GFP-I15-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLCKO_Luciferase_sgRNA

    Plasmid

    #74190

    Purpose

    lentiviral vector expressing sgRNA targeting Luciferase

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Luciferase sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-Ef1a-fDIO-GCaMP6f

    Plasmid

    #128315

    Purpose

    Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

    Depositor

    Patricia Jensen

    Article

    Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

    Has Service

    AAV1

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHD-SPARC2-S-LexA::p65

    Plasmid

    #133564

    Purpose

    Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge