159,117 results

1. pEGFP-C1-FLAG-Ku70

   Plasmid

   #46957

   Purpose

   Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human SERCA2a (pcDNA3.1+)

   Plasmid

   #75187

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   David MacLennan, Jonathan Lytton

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY2PHR(W349R)-mCherry

   Plasmid

   #75370

   Purpose

   Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

   Insert

   CRY2 residues 1-498 (CRY2 Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W349R

   Promoter

   CMV

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_CTNNB1_WT

   Plasmid

   #81734

   Purpose

   Gateway Donor vector containing CTNNB1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYZ174

   Plasmid

   #98421

   Purpose

   Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CMV-GFP-Sox11

   Plasmid

   #120387

   Purpose

   over expression of sox11 using infection

   Depositor

   Igor Ulitsky

   Article

   Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

   Insert

   sox11 (Sox11 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-Sox2

   Plasmid

   #101761

   Purpose

   Sox2 promoter reporter

   Depositor

   Yuh-Shan Jou

   Article

   Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

   Insert

   SRY-box 2 promoter (SOX2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. hTLR4

   Plasmid

   #13086

   Depositor

   Ruslan Medzhitov

   Insert

   TLR4 (TLR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-FLAG-Tapasin

   Plasmid

   #141308

   Purpose

   Mammalian expression of FLAG-tagged Tapasin

   Depositor

   Erik Procko

   Article

   McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

   Insert

   TAPBP (TAPBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0317 HvsCas7-11 mammalian expression

    Plasmid

    #172511

    Purpose

    Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGreenII 0000 (No LUC)

    Plasmid

    #44465

    Depositor

    Roger Hellens

    Type

    Empty backbone

    Use

    Plant transformation

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV/D374Y-hPCSK9

    Plasmid

    #58379

    Purpose

    Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Insert

    proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D374Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNCX myr Akt1

    Plasmid

    #17245

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    Myr

    Expression

    Mammalian

    Mutation

    Constitutively active

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1322 HPV-16 E6

    Plasmid

    #8642

    Depositor

    Peter Howley

    Article

    Münger et al J Virol. 1989 Oct . 63(10):4417-21.

    Insert

    HPV-16 E6 (E6 Human papillomavirus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL410_INS421

    Plasmid

    #49057

    Purpose

    Expresses luciferase under control of human insulin gene promoter

    Depositor

    Kevin Ferreri

    Article

    Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

    Insert

    human INS promoter (INS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    INS promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1/MPL-HA

    Plasmid

    #204530

    Purpose

    Mammalian expression of human MPL-HA

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    MPL-HA (MPL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 HA-Sumo1 WT

    Plasmid

    #48966

    Purpose

    Expresses HA-Sumo1 WT in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. p15TV-L

    Plasmid

    #26093

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. TFORF2509

    Plasmid

    #144387

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EP300 (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hSyn INTRON mScarlet-CAAX2 WPRE

    Plasmid

    #236232

    Purpose

    AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mScarletI

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEND-418_Cacnes_LysA

    Plasmid

    #225607

    Purpose

    Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

    Depositor

    Marc Güell

    Article

    Guillermo et al Cell Systems, 2025

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP

    Plasmid

    #228359

    Purpose

    RfxCas13d (NLS-RfxCas13d-NLS) with 2A-EGFP and 2A-mCD4 for RNA knockdown

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    2A-EGFP, 2A-mCD4, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228362

    Purpose

    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. qTAG-C-mStayGold-Puro-CLTC

    Plasmid

    #227314

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CLTC Homology Arms flanking a mStayGold-2A-Puro Cassette (CLTC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. qTAG-C-Solo-mStayGold-PEX3

    Plasmid

    #227304

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PEX3 Homology Arms flanking a mStayGold Tag (PEX3 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. qTAG-N-Solo-mStayGold-RAB7A

    Plasmid

    #227298

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the RAB7A locus. For endosome visualization. To be co-transfected with sgRNA plasmid px330-RAB7A (Addgene #227297)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    RAB7A Homology Arms flanking a mStayGold Tag (RAB7A Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid

    #227325

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. qTAG-C-mStayGold-Puro-PXN

    Plasmid

    #227317

    Purpose

    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. qTAG-AAVS1-Ef1a-Blast

    Plasmid

    #227267

    Purpose

    Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pX330-RAB7A

    Plasmid

    #227297

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-haA3A-CBE-G-N

    Plasmid

    #220898

    Purpose

    AAV genome encoding the N-terminal of haA3A-CBE-G

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-N
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-haA3A-CBE-G-C

    Plasmid

    #220899

    Purpose

    AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

    Depositor

    Dali Li

    Article

    Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

    Insert

    haA3A-CBE-G-C
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. qTAG-C-moxGFP-Zeo-H2BC11

    Plasmid

    #207759

    Purpose

    Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pPBpuro-EGFR(M721)

    Plasmid

    #197379

    Purpose

    Encoding human ErbB1 mutant.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    EGFR mutant (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lysine 721 to Methionine

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF0471

    Plasmid

    #142635

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KAT7 (KAT7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF0470

    Plasmid

    #142270

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KAT7 (KAT7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KTK_002

    Plasmid

    #180527

    Purpose

    Entry vector containing J23104 promoter

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    J23104 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMT3928

    Plasmid

    #113331

    Purpose

    control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge