164,223 results

1. pFLAG-GATA2

   Plasmid

   #1418

   Depositor

   Gokhan Hotamisligil

   Article

   Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

   Insert

   GATA-2 (GATA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gluc-LHCGR

   Plasmid

   #221781

   Purpose

   Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

   Depositor

   Adolfo Rivero-Muller

   Article

   Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

   Insert

   LHCGR (LHCGR Human)
   

   Use

   Tags

   Gaussia Luciferase and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT05

   Plasmid

   #223377

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-SpCas9-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex-1 GFPNanobody_SNAP

   Plasmid

   #82711

   Purpose

   Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   GFP Nanobody
   

   Use

   Tags

   6xHis Purification tag, FLAG purification tag, an…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYLCRISPR/Cas9Pubi-B

   Plasmid

   #66188

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available Since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

   Plasmid

   #183776

   Purpose

   AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   CAG

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG26

   Plasmid

   #226743

   Purpose

   Expresses a reporter for a mitochondrial intermembrane space protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Halo-OPTN

   Plasmid

   #187900

   Purpose

   Mammalian cell expression of Halo-tagged OPTN

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLdCN2

    Plasmid

    #125186

    Purpose

    Expresses Streptococcus pyogenes Cas9 (SpCas9) and two gRNAs in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al Methods Mol Biol. 2020;2116:199-224. doi

    Insert

    gRNA and Cas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEmerald-C1

    Plasmid

    #53975

    Purpose

    Localization: C1 Cloning Vector, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. PX458-3xHA-en31FnCas9

    Plasmid

    #201949

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en31FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    G1243T, E1369R, E1603H on FnCas9

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-FKBP:mCherry-KRas4Bc30

    Plasmid

    #72900

    Purpose

    Membrane-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    GTPase K-Ras (KRAS Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 158..188 (fragment 1..157 deleted)

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIZ GFP PODXL B WT

    Plasmid

    #202414

    Purpose

    Expression of GFP-tagged PODXL (isoform B) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PET-eSpCas9-NLS-6xHis

    Plasmid

    #207379

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS2165_Nme2-ABE8e-i8

    Plasmid

    #201518

    Purpose

    Mammalian expression of domain inlaid Nme2-ABE8e-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro-cGASsh4

    Plasmid

    #127646

    Purpose

    Knock-down of human cGAS

    Depositor

    Nicolas Manel

    Article

    Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

    Insert

    cGAS shRNA (CGAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-EGFP-Cdc42-T17N

    Plasmid

    #12976

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 DN (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-Cytb5

    Plasmid

    #182579

    Purpose

    Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Cytb5
    

    Use

    Tags

    Cytb5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF1808

    Plasmid

    #143849

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX18 (TBX18 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid

    #169248

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO.1 mito-TagRFP-T

    Plasmid

    #174543

    Purpose

    labels mitochondria with TagRFP-T

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    MTS-tagRFP-T
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

    Plasmid

    #71720

    Purpose

    Expressing an mRuby-tagged TIR1 in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
    

    Use

    Tags

    mRuby

    Expression

    Worm

    Mutation

    codon-optimized for expression in C. elegans; Two…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCFJ151 - ttTi5605_MCS

    Plasmid

    #19330

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    ttTi5605 targeting region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-DN-hCUL3-FLAG

    Plasmid

    #15820

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin3 (CUL3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N418

    Promoter

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. gh92

    Plasmid

    #106763

    Purpose

    expression of gRNA targeting FUT8

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT8 (FUT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAG Lenti CMV EGFP Puro

    Plasmid

    #236084

    Purpose

    Lentiviral backbone expressing EGFP, control for empty backbone 236083

    Depositor

    Melina Fan

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-THRA-RFP-EGFP

    Plasmid

    #228537

    Purpose

    Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

    Depositor

    Markus Schuelke

    Article

    Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

    Insert

    Thyroid Hormone Receptor Alpha (THRA Human)
    

    Use

    Tags

    eGFP and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. VCP(R155H)-EGFP

    Plasmid

    #23972

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R155H

    Promoter

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSQT1313

    Plasmid

    #53370

    Purpose

    multiplex gRNA expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    None
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFS181

    Plasmid

    #12536

    Depositor

    Nick Rhind

    Article

    Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

    Insert

    human equilibrating nucleoside transporter 1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. HaloTag-LaminA

    Plasmid

    #231894

    Purpose

    Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    LaminA (LMNA Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCK017 pDEST exorh:EGFP

    Plasmid

    #195983

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TFORF2638

    Plasmid

    #142344

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge