We narrowed to 3,314 results for: GCT

1. pBSU6-shTOM40.3

   Plasmid

   #89796

   Purpose

   Expresses shRNA against TOM40.

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Tom40
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PM-ST1!TA

   Plasmid

   #48653

   Purpose

   Bacterial ST1 crRNA expression: targets ST1 to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial ST1 crRNA to prototspacer A
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23100 promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQdCas12a.luxR(mut)-sggfp(B)

   Plasmid

   #236041

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQdCas12a.luxR(mut)-sggfp(A)

   Plasmid

   #236039

   Purpose

   The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDual_dsCas9_Venus_hs_NRF2

   Plasmid

   #214692

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2

   Depositor

   Kevin Janes

   Insert

   dgRNA_NRF2 (NFE2L2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCHW00015

   Plasmid

   #222446

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containig VP64-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP64-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCHW00014

   Plasmid

   #222445

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing VP16-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   VP16-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. msHook2 g1 lentiCRISPRv2-mCherry

   Plasmid

   #218652

   Purpose

   Knockout vector for mouse Hook2

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Hook2 (Hook2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. msHook2 g2 lentiCRISPRv2-mCherry

   Plasmid

   #218653

   Purpose

   Knockout vector for mouse Hook2

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Hook2 (Hook2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGE010-gR085

    Plasmid

    #188554

    Purpose

    Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    gR085
    

    Use

    CRISPR; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGE_En03-gR085

    Plasmid

    #188552

    Purpose

    Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

    Depositor

    Yutaka Kodama

    Article

    Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

    Insert

    gR085
    

    Use

    CRISPR; Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CB0026

    Plasmid

    #162285

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0CB0246

    Plasmid

    #162286

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0CM0027

    Plasmid

    #162309

    Purpose

    Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPQD0KN0283

    Plasmid

    #162288

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0KN0282

    Plasmid

    #162287

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0KN0244

    Plasmid

    #162282

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPQD0KN0024

    Plasmid

    #162281

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-FLEX-SaCas9-U6-sgDcc

    Plasmid

    #159906

    Purpose

    Mutagenesis of Dcc

    Depositor

    Larry Zweifel

    Insert

    Dcc (Dcc Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSUI-SynP-CAPsg

    Plasmid

    #154344

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CAP sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PX458_SIX4_iso1_1

    Plasmid

    #128247

    Purpose

    Encodes gRNA for 3' target of human SIX4_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SIX4_iso1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PX458_ZBTB49_iso1_2

    Plasmid

    #128251

    Purpose

    Encodes gRNA for 3' target of human ZBTB49_iso1

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZBTB49_iso1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLHDR_R1

    Plasmid

    #124209

    Purpose

    gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pELF4.1.0-gDNA

    Plasmid

    #112467

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELF4

    Depositor

    Kevin White

    Insert

    ELF4 (ELF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hROSA26 CRISPR-pX330

    Plasmid

    #105927

    Purpose

    hROSA26 targeting CRISPR plasmid

    Depositor

    Masato Kanemaki

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    hROSA26 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. miniCMV-iRFP670-24xMS2

    Plasmid

    #238915

    Purpose

    Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

    Insert

    iRFP670-24xMS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. miniCMV-tdTomado-tDeg

    Plasmid

    #185402

    Purpose

    tdTomado-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    tdTomado-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. miniCMV-EGFP-tDeg

    Plasmid

    #185400

    Purpose

    EGFP-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    EGFP-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMLS597

    Plasmid

    #188884

    Purpose

    dpy-10 sgRNA expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    PU6(K09B11.12)::dpy-10 sgRNA (dpy-10 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCR-Zeo

    Plasmid

    #158709

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Tet-pLKO-FOXM1-shRNA-#3

    Plasmid

    #89500

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pgRNA

    Plasmid

    #215560

    Purpose

    Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCHW110033

    Plasmid

    #222441

    Purpose

    Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GEARBOCS-SPARCL1-N-mCherryTag

    Plasmid

    #218186

    Purpose

    To tag Hevin with mCherry at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCR-Hyg

    Plasmid

    #158708

    Purpose

    For cloning and constitutive expression of crRNAs in mycobacterium

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    crRNA
    

    Use

    CRISPR and Synthetic Biology; Mycobacteria expres…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Hsp60

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiCRISPR - CTRL sgRNA

    Plasmid

    #70662

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAGT10446

    Plasmid

    #192353

    Purpose

    L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-t35S_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCHW100002

    Plasmid

    #222440

    Purpose

    Level 0, CDS module (AATG-GCTT) containing rice OsL5.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    OsL5 (Oryza sativa)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge