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Showing: 1401 - 1450 of 6893 results

  5. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

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  8. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Article
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    50834-plasmid-map-sequence-id-76270

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  9. pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid
    #126080
    Purpose
    sCre-dependent ChR2(H134R)-eYFP with the sLox sites in the reverse orientation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)-eYFP
    Use
    AAV
    Tags
    eYFP
    Expression
    Mutation
    H134R
    Promoter
    Ef1a
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    126080-plasmid-map-sequence-id-245075

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  20. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid
    #117139
    Purpose
    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP
    Depositor
    Kosuke Yusa
    Article
    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste
    Insert
    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    modified BFP, modified Dmap1 CDS
    Promoter
    human U6 promoter, human EF1a promoter
    Available since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    117139-plasmid-map-sequence-id-243795

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  27. pAAV-Ef1a-Con/Fon-Synaptophysin-10xMyc-WPRE

    Plasmid
    #237446
    Purpose
    Intersectional expression of Myc-tagged synaptophysin in the presence of Cre and Flp
    Depositor
    Silvia Arber
    Insert
    Con/Fon-Synaptophysin-10xMyc
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    237446-plasmid-map-sequence-id-470110

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  28. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid
    #234952
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234952-plasmid-map-sequence-id-467733

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  29. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234953
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234953-plasmid-map-sequence-id-467731

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  30. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234954
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234954-plasmid-map-sequence-id-467729

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  32. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid
    #234951
    Purpose
    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    234951-plasmid-map-sequence-id-467430

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  34. pAAV-EF1a-mHFE(H67D)-IRES-GFP-WPRE-pA

    Plasmid
    #233040
    Purpose
    To express mouse homeostatic iron regulator (HFE)(H67D) from an EF1a promoter. It also encodes GFP after an IRES sequence
    Depositor
    Jonathan Ploski
    Insert
    mouse homeostatic iron regulator with H67D mutation (Hfe Mouse)
    Use
    AAV
    Tags
    GFP
    Expression
    Mutation
    H67D
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    233040-plasmid-map-sequence-id-465998

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  35. pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233033
    Purpose
    To Express HA tagged DjCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233033-plasmid-map-sequence-id-464626

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  36. pAAV-CMV-intron-hfCas13d-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233034
    Purpose
    To Express HA tagged hfCas13d the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233034-plasmid-map-sequence-id-463768

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  37. pAAV-CMV-intron-CasRx-pA/EF1a-mCherry-WPRE-pa

    Plasmid
    #233032
    Purpose
    To Express HA tagged CasRX the CMV promoter. Also encodes a mCherry gene outside of the viral genome
    Depositor
    Jonathan Ploski
    Insert
    CasRx and mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233032-plasmid-map-sequence-id-463767

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  41. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

    Plasmid
    #226590
    Purpose
    Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.
    Depositor
    D. James Surmeier
    Insert
    PSAML-141F,Y115F-GlyR
    Use
    AAV
    Tags
    P2A-Fusion Red
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    226590-plasmid-map-sequence-id-450965

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Showing: 1401 - 1450 of 6893 results