174,131 results
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Plasmid#153303Purposeexpress CARPID dCasRx-BASU fusion protein in mammalian cellsDepositorInsertdCasRx-BASU
UseCRISPR and LentiviralExpressionMammalianMutationR239A/H244A/R858A/H863A in RfxCas13dPromoterEF-1aAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-BFP
Plasmid#138272PurposeExpression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editorDepositorInsertBFP H66 PAM
UseCRISPRExpressionMammalianMutationH66, PAM motifPromoterEf1aAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag
Plasmid#132403Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP3Kv-WPRE
Plasmid#132332Purposeexpresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vectorDepositorInsertASAP3Kv
UseAAVExpressionMammalianPromoterhSynapsinAvailable SinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-mEosEM
Plasmid#132708Purposeapplication in Correlative light-electron microscopyDepositorInsertmEosEM
ExpressionBacterialAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorInsertmgSrtA
ExpressionMammalianAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneTagsGSTExpressionMammalianAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE60-RRF-His
Plasmid#124132PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianAvailable SinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
TagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-2A-mTurquoise2
Plasmid#98885PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmNeonGreen-2A-mTurquoise2
ExpressionMammalianPromoterCMVAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianPromoterEF1aAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Synapsin-cyto-iATPSnFR1.0
Plasmid#102556Purposeexpresses cytoplasmic ATP sensor in neuronsDepositorInsertiATPSnFR1.0
UseAAVAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTP288
Plasmid#113460Purposehuman Flag tagged Nbs1 in baculovirus transfer vectorDepositorAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-14-3-3 eta
Plasmid#116887PurposeExpresses HA-tagged human 14-3-3 eta in mammalian cellsDepositorAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-LC3-YFP
Plasmid#99571PurposeLentiviral expression of YFP-tagged LC3DepositorAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
TagsMBPExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyExpressionYeastPromoterPGK1, TDH3, TIP1, and ZEO1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralExpressionMammalianAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-gate-3xHA-pGK-PUR
Plasmid#107393PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTags3xHAExpressionMammalianAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX551-CMV-SpCas9
Plasmid#107024PurposeAAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang labDepositorInsertSpCas9
UseAAV and CRISPRExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GfaABC1D.PI.Cre.SV40
Plasmid#105603PurposeAAV expression of Cre from GfaABC1D promoterDepositorInsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterGfaABC1DAvailable SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
TagsmCherryExpressionBacterial and YeastAvailable SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-EYFP
Plasmid#104052PurposeAn AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoterDepositorHas ServiceAAV PHP.V1InsertEYFP
UseAAVPromoterCAGAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090
Plasmid#103869PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available SinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC101_TIMER
Plasmid#103057Purposelow copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
ExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWUR790
Plasmid#101723PurposeSynthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgoDepositorInsertPfAgo
TagsStrep(II)-tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A-dCas9
Plasmid#100090PurposeDNMT3A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertDNMT3A (DNMT3A Human)
UseCRISPRTags3XFLag-NLS-DNMT3A-dCas9-NLSExpressionMammalianMutationincludes aa 602-912PromoterCMVAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
VN-Tau (P301L)
Plasmid#87634Purposeexpresses hTau fused to the N-terminal part of VenusDepositorInsertMAPT (MAPT Human)
TagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only