We narrowed to 18,127 results for: Mut

1. hCdc20 (1-120)

   Plasmid

   #8509

   Depositor

   Joan Ruderman

   Article

   Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

   Insert

   hCdc20 (1-120) (CDC20 Human)
   

   Use

   Retroviral

   Tags

   His

   Expression

   Mammalian

   Mutation

   Cdc20 N-terminus. Dominant Negative mutant.

   Promoter

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pENTR-L3-Csy4-Cas9n-EGFP-L2

   Plasmid

   #141046

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9n-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. raACR-pmCerulean3-N1

   Plasmid

   #204959

   Purpose

   Expression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   raACR
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+enhancer)

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CALCOCO1 W47A-mycDDK

   Plasmid

   #137769

   Purpose

   CALCOCO1 LIR mutant

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   CALCOCO1 W47A
   

   Use

   Tags

   mycDDK

   Expression

   Mammalian

   Mutation

   W47A

   Promoter

   CMV

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MECP2 - R133C in pENTR

   Plasmid

   #216594

   Purpose

   gateway cloning donor vector containing MECP2 ASD mutation

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   R133C

   Promoter

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEB2-TagRFP

   Plasmid

   #103997

   Purpose

   Plasmid encoding TagRFP

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   TagRFP
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, K67R, …

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDYSCKO-ADE1

   Plasmid

   #120264

   Purpose

   pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

   Depositor

   Christian Landry

   Article

   Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

   Insert

   pDYSCKO cassette-ADE1 targeting guide
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   SNR52

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-Wnt8A

   Plasmid

   #35916

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt8A (Wnt8a Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Silent Mutation A (600) --> G

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUG-UBC8

    Plasmid

    #104673

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    AtUBC8 (UBC8 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

    Plasmid

    #216385

    Purpose

    Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Norrin (NDP Human)
    

    Use

    Baculovirus

    Tags

    GP64 signal sequence-MBP-3C and Rho1D4

    Expression

    Insect

    Mutation

    C93A/C95A/C131A

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJZC518

    Plasmid

    #62277

    Purpose

    Yeast dCas9 expression plasmid

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Has mutations in the RuvC1 and HNH domains to ren…

    Promoter

    pTdh3

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCSG7-D16A-NmeCas9

    Plasmid

    #71476

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-D16A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A

    Promoter

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMito-dCherry-FRB

    Plasmid

    #186573

    Purpose

    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Dark MitoTrap
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of "K70N" to render mCherry no…

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNb4OMT-A53M

    Plasmid

    #216232

    Purpose

    Expresses a mutant Norbelladine 4'-O-Methyltransferase

    Depositor

    Andrew Ellington, Simon d'Oelsnitz

    Article

    d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

    Insert

    Nb4OMT-A53M
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    A53M

    Promoter

    P150

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMCSG7-H588A-NmeCas9

    Plasmid

    #71477

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-H588A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H588A

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-Cas9-tdTomato

    Plasmid

    #141013

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lsg-LMNA-1 + 2

    Plasmid

    #199278

    Purpose

    nicking sgRNA to induce LMNA (c.1824C > T) mutation using PE3

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LMNA nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA5/FRT/TO_Lifeact_STf

    Plasmid

    #182011

    Purpose

    CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lifeact_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA5/FRT/TO_TOMM20-STf

    Plasmid

    #182002

    Purpose

    CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    TOMM20-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV HDM2(NES-)

    Plasmid

    #12087

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nuclear-export sequence mutant (NES-): L205A, I20…

    Promoter

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

26. Nephrin

    Plasmid

    #186134

    Purpose

    For expression of recombinant nephrin in bacteria cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    NPHS1 (NPHS1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Amino acid residues 1174-1223, with mutations: Y1…

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEB1-mCerulean ME

    Plasmid

    #103971

    Purpose

    Plasmid encoding mCerulean ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean ME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_N_ZIC3

    Plasmid

    #167809

    Purpose

    Expresses ZIC3 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-C1-hRabin8 F201A

    Plasmid

    #26728

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    F201A: GEF domain mutant

    Promoter

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pGFP-PTEN G129E

    Plasmid

    #50523

    Depositor

    Kenneth Yamada

    Article

    Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G129E

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pAc5.1B-EGFP-DmGIGYF_AH

    Plasmid

    #148918

    Purpose

    Insect Expression of DmGIGYF

    Depositor

    Elisa Izaurralde

    Article

    Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

    Insert

    DmGIGYF (Gyf Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSCV-Pidd1(T788D)

    Plasmid

    #60530

    Purpose

    Retroviral expression of Pidd1 T788D phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Aspartic acid*

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pEGFP-C1-hRabin8 L196A

    Plasmid

    #26727

    Depositor

    Keith Mostov

    Article

    Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L196A: GEF domain mutant.

    Promoter

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

35. Syn:eGFP-CaM-uTEV1Δ(220-242)

    Plasmid

    #135463

    Purpose

    Neuronal expression of uFLARE protease component

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    eGFP-CaM-uTEV1Δ
    

    Use

    AAV

    Tags

    eGFP, V5

    Expression

    Mammalian

    Mutation

    S219V mutation improves stability and S153N impro…

    Promoter

    Synapsin

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 1342 pSG5L HA FKHRL1 AAA

    Plasmid

    #10711

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHRL1 AAA (FOXO3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AAA mutant (see map)

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pET14b PHAS-I F113A

    Plasmid

    #15680

    Depositor

    John Lawrence

    Article

    Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

    Insert

    PHAS-I F113A (Eif4ebp1 Rat)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F113A mutation.

    Promoter

    Available Since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

38. MAC_N_ETV7

    Plasmid

    #167756

    Purpose

    Expresses ETV7 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV7 (ETV7 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX330A_D10A-1x6

    Plasmid

    #58776

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET23b-Casp9 C287A-His

    Plasmid

    #11830

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 catalytic mutant (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C287A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only