2,172 results

Showing: 61 - 90 of 2172 results

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-gfap(Intron1/5'/Exon1-zebrafish)

Plasmid

#39761

Depositor

Pamela Raymond

Article

Bernardos et al Gene Expr Patterns. 2006 Oct;6(8):1007-1

Insert

zebrafish gfap promoter- EGFP (gfap Zebrafish)

Use

Zebrafish expression

Promoter

gfap

Available Since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:EGFP

Plasmid

#118850

Purpose

Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

mitfa: EGFP (mitfa Synthetic, Zebrafish)

Use

Zebrafish expression, tol6

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -1

Plasmid

#173887

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCK029 exorh:EGFP

Plasmid

#195980

Purpose

Expression construct

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:EGFP

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-hsp70I:QFGal4-SV40pA

Plasmid

#155120

Purpose

Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

hsp70l:QFGal4 (hsp70l Budding Yeast, Zebrafish, Neurospora crassa)

Use

Zebrafish expression

Promoter

hsp70l

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Ubi:Zebrabow

Plasmid

#118219

Purpose

For ubiquitous expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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Tol2-ngn1-Trpa1b-2A-mCherry

Plasmid

#106425

Purpose

For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway

Tags

2A-mCherry

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only
pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

Plasmid

#162566

Purpose

Used for the dual color labeling of cPLA2 and LaminB1 expression.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

cytosolic phospholipase a2 (pla2g4aa Zebrafish)
Lamin B1 (lmnb1 Zebrafish)

Tags

EGFP, P2A, and mKate2

Expression

Mammalian

Promoter

CMV and SV40

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; control_gRNA

Plasmid

#199336

Purpose

pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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hsp70l: hAPOB48-mCherry.

Plasmid

#140639

Purpose

Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.

Depositor

Steven Farber

Article

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Inserts

hsp70l (hsp70l Zebrafish)
APOB (APOB Human)

Use

Zebrafish expression

Tags

mCherry

Mutation

APOB48: amino acid 1-2180

Promoter

hsp70l

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_ect2_cmlc2-nmKate

Plasmid

#238410

Purpose

Drives expression of 3 different gRNAs targeting ect2, and expression of nuclear mKate in cardiomyocytes

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting ect2

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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slc1a3b:myrGFP-P2A-H2AmCherry

Plasmid

#170208

Purpose

Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGFP-P2A-H2AmCherry

Promoter

slc1a3b

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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Tg(mpeg1:LifeAct-mRuby)

Plasmid

#166977

Purpose

labels F-actin in macrophages

Depositor

Anna Huttenlocher

Article

Barros-Becker et al J Cell Sci. 2017 Nov 15;130(22):3801-380

Inserts

mpeg1 (mpeg1.1 Zebrafish)
LifeAct (actb1 Frog)

Tags

Lifeact-mRuby and mRuby

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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ZE32 Prickle-1-GFP-pCS2+

Plasmid

#17073

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Tags

GFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p3E ubb polyA

Plasmid

#195977

Purpose

Gateway p3E vector

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubb:polyA (ubb Zebrafish)

Use

Other

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Article

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-kdrl

Plasmid

#78687

Purpose

kdrl promoter for Gateway cloning

Depositor

Jau-Nian Chen, Sarah Childs

Article

Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

Insert

kdrl promoter (kdrl Zebrafish)

Use

Unspecified

Promoter

kdrl

Available Since

June 5, 2016

Availability

Academic Institutions and Nonprofits only

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p5E_unc503

Plasmid

#64020

Purpose

5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45b

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

muscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)

Use

Gateway

Promoter

503-bp promoter of zebrafish unc-45b

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pPRISM-Cre-cmlc2-TagBFP

Plasmid

#117792

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

Cre

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3194

Plasmid

#41368

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Left (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
TAL3195

Plasmid

#41369

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tfec-Right (tfec Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2012

Availability

Academic Institutions and Nonprofits only
pDEST-Tol2-PA2-CMV-AB-mCh

Plasmid

#160435

Purpose

Expresses human Amyloid Beta-mCherry in Zebrafish

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Human Amyloid Beta peptide (1-42) (APP Zebrafish, Human)

Use

Zebrafish expression

Tags

mCherry

Promoter

CMV

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pUAS-Ncad-GFP

Plasmid

#35986

Depositor

James Jontes, Stephen Smith

Article

Jontes et al J Neurosci. 2004 Oct 13;24(41):9027-34.

Insert

Ncad (cdh2 Zebrafish)

Use

Gal4 inducible

Tags

EGFP

Promoter

14xUAS-E1b

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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bactin2:loxP-STOP-loxP-DsRed-express

Plasmid

#68797

Purpose

For making transgenic fish

Depositor

David Traver

Article

Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

Inserts

beta-actin promoter
loxp-stop-codon-loxp-dsred

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-gfap

Plasmid

#82401

Purpose

Gateway-compatible zebrafish gfap promoter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

gfap promoter

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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2,172 results