We narrowed to 471 results for: 1183

1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

   Plasmid

   #89061

   Purpose

   AAV-gRNA targeting the murine Ldlr gene

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HCT41_rAAV-eHGT_1183m-minBglobin-SYFP2-WPRE3-BGHpA

   Plasmid

   #223918

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Tanya Daigle, Allen Institute for Brain Science

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pME-myr-mTagBFP2-Flag (JDW 1183)

   Plasmid

   #224492

   Purpose

   Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   myr-mTagBFP2-Flag
   

   Use

   Gateway cloning

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839)

   Plasmid

   #163509

   Purpose

   AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications.

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Has Service

   AAV PHP.eB

   Insert

   H2B-SYFP2
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Mammalian expression: Z-lock cofilin dark state mutant

   Plasmid

   #141136

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Mammalian

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Z-lock αTAT

   Plasmid

   #175290

   Purpose

   light-controlled analog of the alpha-tubulin acetylase αTAT

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Z-lock αTAT
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   αTAT (236 amino acids)

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. E. coli expression: Z-lock cofilin dark state mutant

   Plasmid

   #141135

   Purpose

   a control for optogenetic cofilin analog

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Bacterial

   Mutation

   D3 deletion, I32F in Zdk2

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Anti-Clcn4 [N222/6R]

   Plasmid

   #114474

   Purpose

   Mammalian Expression Plasmid of anti-Clcn4 (Human). Derived from hybridoma N222/6.

   Depositor

   James Trimmer

   Article

   Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Clcn4 (Homo sapiens) recombinant mouse monoclonal antibody (CLCN4 Mouse)
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   dual CMV

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF5/FRT-DEST EVC2ala4-Flag

    Plasmid

    #41008

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

    Plasmid

    #41011

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMCh-1498

    Plasmid

    #82427

    Purpose

    Plasmid for expression of NHA-tagged Nematostella vectensis GW182 11W11A in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 11W11A
    

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    Trp 1167, 1183, 1227, 1309, 1391, 1453, 1574, 158…

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMCh-1522

    Plasmid

    #82428

    Purpose

    Plasmid for expression of GST-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    EF1A

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCh-1526

    Plasmid

    #82429

    Purpose

    Plasmid for expression of NHA-tagged C-terminal region of Nematostella vectensis GW182 11W11A (aa 1159-1698) in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182 CED 11W11A
    

    Tags

    NHA

    Expression

    Mammalian

    Mutation

    amino acids 1-1158 of nvGW182 were deleted and Tr…

    Promoter

    CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LgBiT-AR/AR-SmBiT Bidirectional Vector

    Plasmid

    #237155

    Purpose

    Express LgBiT-AR/AR-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    AR (AR Human)
    AR (AR Human)
    

    Tags

    LgBiT and SmBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. linker-P2A-mem-mScarlet-ORF-1

    Plasmid

    #241183

    Purpose

    Middle entry vector for mini-Golden subcloning platform; mScarlet

    Depositor

    Junsu Kang

    Insert

    mScarlet
    

    Use

    Mini-golden

    Mutation

    TCGC linker

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-link-sfGFP-FLAG:Kan

    Plasmid

    #211833

    Purpose

    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    sfGFP-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-link-mVenus-FLAG:Kan

    Plasmid

    #211830

    Purpose

    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mVenus-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-link-PAmCherry-FLAG:cat

    Plasmid

    #211832

    Purpose

    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAmCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC19-link-PAGFP-FLAG:Kan

    Plasmid

    #211834

    Purpose

    Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    PAGFP-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAc5.1C-FLuc-V5His6

    Plasmid

    #21183

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

    Insert

    Firefly Luciferase
    

    Use

    Drosophila expression

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

23. pBabe(LTR)-Cytochrome C-GFP

    Plasmid

    #41183

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C-GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21a-LuxT

    Plasmid

    #51183

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    LuxT
    

    Use

    Synthetic Biology

    Tags

    6x-his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSGE_889_Nhe2_EcR-to-Ets96B_RB

    Plasmid

    #71183

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    889_Nhe2_EcR-to-Ets96B_RB
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    DSCP Drosophila synthetic core promoter

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQCXI Neo DsRed-LC3-GFP

    Plasmid

    #31183

    Depositor

    David Sabatini

    Article

    Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

    Insert

    DsRed-LC3-GFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI) promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRRL-VinculinTS

    Plasmid

    #111830

    Purpose

    Vinculin tension sensor in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (VCL Chicken)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

    Plasmid

    #211837

    Purpose

    Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRRL-Vinculin-Venus

    Plasmid

    #111833

    Purpose

    Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    Vinculin-Venus (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    VenusA206K

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDGB3_alpha1-AtUBQ10:GFP-T35S

    Plasmid

    #211836

    Purpose

    Plant transient expression cassette for GFP (control) under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    GFP control
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Promoter

    UBIQUITIN10

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMB-APr

    Plasmid

    #61183

    Purpose

    Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFLAG rat Antizyme 1

    Plasmid

    #11183

    Depositor

    Bruce Zetter

    Article

    Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

    Insert

    AZ1 (Oaz1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMK349 (BSD-P2A-mAID-mCherry)

    Plasmid

    #121183

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-mCherry
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRRL-VinculinTS-I997A

    Plasmid

    #111832

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A) (VCL Chicken)
    

    Use

    Lentiviral

    Mutation

    changed vinculin isoleucine 997 to alanine

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRRL-VinculinTS-A50I

    Plasmid

    #111831

    Purpose

    Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (A50I) (VCL Chicken)
    

    Use

    Lentiviral

    Mutation

    changed vinculin alanine 50 to isoleucine

    Promoter

    CMV

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S

    Plasmid

    #211839

    Purpose

    Plant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191183

    Purpose

    Expression of PPIB sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:PPIB.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOVC1_AtPHYB-S_X

    Plasmid

    #131183

    Purpose

    Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    AtPHYB-S
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pQFmcs-2H12.D11

    Plasmid

    #221183

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Expression

    Bacterial

    Promoter

    PQ5 (cumate-inducible)

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJG363

    Plasmid

    #91183

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRRL-VinculinTS-S1033A

    Plasmid

    #211835

    Purpose

    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinTS-S1033A (VCL Synthetic, Chicken)
    

    Use

    Lentiviral

    Mutation

    mutated vinculin serine 1033 to alanine (S1033A),…

    Promoter

    CMV

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCEP4-Q769gp160-QES.i03.V255M

    Plasmid

    #111838

    Purpose

    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations A200E;F…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCEP4-BaLgp160-QES.i01.c01

    Plasmid

    #111839

    Purpose

    Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations T49D;P1…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCEP4-Q842gp160-QES.i04.I181L

    Plasmid

    #111837

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations P124D;I…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

    Plasmid

    #101183

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    M694A/Q695A/H698A/Q926A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid

    #141183

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEUI(+)

    Plasmid

    #111834

    Purpose

    For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

    Depositor

    Hyunju Ro

    Article

    Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

    Insert

    GAL4-minimal VP16-EcR (EcR Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV, SV40, 10XUAS

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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